Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect
Quercetin is a bioactive component that is capable of having therapeutic potential in the prevention of different noncommunicable chronic diseases (NCDs). However, it presents instability in the gastrointestinal tract in addition to low bioavailability. One way to overcome the limitations of quercet...
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MDPI AG
2022-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/5/888 |
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| author | Jocelyn C. Ayala-Fuentes Melissa Zulahi Gallegos-Granados Luis Jesús Villarreal-Gómez Marilena Antunes-Ricardo Daniel Grande Rocio Alejandra Chavez-Santoscoy |
| author_facet | Jocelyn C. Ayala-Fuentes Melissa Zulahi Gallegos-Granados Luis Jesús Villarreal-Gómez Marilena Antunes-Ricardo Daniel Grande Rocio Alejandra Chavez-Santoscoy |
| author_sort | Jocelyn C. Ayala-Fuentes |
| collection | DOAJ |
| description | Quercetin is a bioactive component that is capable of having therapeutic potential in the prevention of different noncommunicable chronic diseases (NCDs). However, it presents instability in the gastrointestinal tract in addition to low bioavailability. One way to overcome the limitations of quercetin lies in using nanotechnology for the development of nanoparticles, based on biopolymers, that are capable of being ingestible. Inulin, a fructan-type polysaccharide, acts as a delivery system for the release of quercetin in a target cell, guaranteeing the stability of the molecule. Inulin-coated quercetin nanoparticles were synthesized by the spray dryer method, and four variables were evaluated, namely inulin concentration (5–10% <i>w</i>/<i>v</i>), feed temperature (40–60 °C), inlet temperature (100–200 °C) and outlet temperature (60–100 °C). The optimal conditions were obtained at 10% <i>w</i>/<i>v</i> inulin concentration, with 45 °C feed temperature, 120 °C inlet temperature and 60 °C outlet temperature, and the nanoparticle size was 289.75 ± 16.3 nm in water. Fluorescence microscopy indicated quercetin loading in the inulin nanoparticles, with an encapsulation efficiency of approximately 73.33 ± 7.86%. Inulin-coated quercetin nanoparticles presented effects of inhibition in Caco-2 and HepG2 cells, but not in HDFa cells. The experimental data showed the potential of inulin nanoparticles as transport materials for unstable molecules, in oral administration systems, for the encapsulation, protection and release of quercetin. |
| first_indexed | 2024-03-10T03:08:50Z |
| format | Article |
| id | doaj.art-b1cc18ac804540fba787804fb19f6839 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T03:08:50Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-b1cc18ac804540fba787804fb19f68392023-11-23T12:35:58ZengMDPI AGPharmaceutics1999-49232022-04-0114588810.3390/pharmaceutics14050888Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity EffectJocelyn C. Ayala-Fuentes0Melissa Zulahi Gallegos-Granados1Luis Jesús Villarreal-Gómez2Marilena Antunes-Ricardo3Daniel Grande4Rocio Alejandra Chavez-Santoscoy5Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501 Sur, Monterrey 64849, MexicoFacultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Tijuana 22260, MexicoFacultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Tijuana 22260, MexicoTecnologico de Monterrey, The Institute for Obesity Research, Ave. Eugenio Garza Sada 2501 Sur, Monterrey 64849, MexicoUniv Est Creteil, CNRS, Institut de Chimie et des Matériaux Paris-Est (ICMPE), 2, rue Henri Du-nant, 94320 Thiais, FranceTecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501 Sur, Monterrey 64849, MexicoQuercetin is a bioactive component that is capable of having therapeutic potential in the prevention of different noncommunicable chronic diseases (NCDs). However, it presents instability in the gastrointestinal tract in addition to low bioavailability. One way to overcome the limitations of quercetin lies in using nanotechnology for the development of nanoparticles, based on biopolymers, that are capable of being ingestible. Inulin, a fructan-type polysaccharide, acts as a delivery system for the release of quercetin in a target cell, guaranteeing the stability of the molecule. Inulin-coated quercetin nanoparticles were synthesized by the spray dryer method, and four variables were evaluated, namely inulin concentration (5–10% <i>w</i>/<i>v</i>), feed temperature (40–60 °C), inlet temperature (100–200 °C) and outlet temperature (60–100 °C). The optimal conditions were obtained at 10% <i>w</i>/<i>v</i> inulin concentration, with 45 °C feed temperature, 120 °C inlet temperature and 60 °C outlet temperature, and the nanoparticle size was 289.75 ± 16.3 nm in water. Fluorescence microscopy indicated quercetin loading in the inulin nanoparticles, with an encapsulation efficiency of approximately 73.33 ± 7.86%. Inulin-coated quercetin nanoparticles presented effects of inhibition in Caco-2 and HepG2 cells, but not in HDFa cells. The experimental data showed the potential of inulin nanoparticles as transport materials for unstable molecules, in oral administration systems, for the encapsulation, protection and release of quercetin.https://www.mdpi.com/1999-4923/14/5/888inulinflavonoidnanoparticlequercetin |
| spellingShingle | Jocelyn C. Ayala-Fuentes Melissa Zulahi Gallegos-Granados Luis Jesús Villarreal-Gómez Marilena Antunes-Ricardo Daniel Grande Rocio Alejandra Chavez-Santoscoy Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect Pharmaceutics inulin flavonoid nanoparticle quercetin |
| title | Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect |
| title_full | Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect |
| title_fullStr | Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect |
| title_full_unstemmed | Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect |
| title_short | Optimization of the Synthesis of Natural Polymeric Nanoparticles of Inulin Loaded with Quercetin: Characterization and Cytotoxicity Effect |
| title_sort | optimization of the synthesis of natural polymeric nanoparticles of inulin loaded with quercetin characterization and cytotoxicity effect |
| topic | inulin flavonoid nanoparticle quercetin |
| url | https://www.mdpi.com/1999-4923/14/5/888 |
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