Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MS
This study aimed at an experimental design of response surface methodology (RSM) in the optimization of the dominant volatile fraction of Greek thyme honey using solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). For this purpose, a multiple response opt...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/12/3612 |
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| author | Marinos Xagoraris Alexandra Skouria Panagiota-Kyriaki Revelou Eleftherios Alissandrakis Petros A. Tarantilis Christos S. Pappas |
| author_facet | Marinos Xagoraris Alexandra Skouria Panagiota-Kyriaki Revelou Eleftherios Alissandrakis Petros A. Tarantilis Christos S. Pappas |
| author_sort | Marinos Xagoraris |
| collection | DOAJ |
| description | This study aimed at an experimental design of response surface methodology (RSM) in the optimization of the dominant volatile fraction of Greek thyme honey using solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). For this purpose, a multiple response optimization was employed using desirability functions, which demand a search for optimal conditions for a set of responses simultaneously. A test set of eighty thyme honey samples were analyzed under the optimum conditions for validation of the proposed model. The optimized combination of isolation conditions was the temperature (60 °C), equilibration time (15 min), extraction time (30 min), magnetic stirrer speed (700 rpm), sample volume (6 mL), water: honey ratio (1:3 <i>v</i>/<i>w</i>) with total desirability over 0.50. It was found that the magnetic stirrer speed, which has not been evaluated before, had a positive effect, especially in combination with other factors. The above-developed methodology proved to be effective in the optimization of isolation of specific volatile compounds from a difficult matrix, like honey. This study could be a good basis for the development of novel RSM for other monofloral honey samples. |
| first_indexed | 2024-03-10T10:27:37Z |
| format | Article |
| id | doaj.art-b1d9df43c87a40fbb419383fc04ca434 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:27:37Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b1d9df43c87a40fbb419383fc04ca4342023-11-21T23:52:57ZengMDPI AGMolecules1420-30492021-06-012612361210.3390/molecules26123612Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MSMarinos Xagoraris0Alexandra Skouria1Panagiota-Kyriaki Revelou2Eleftherios Alissandrakis3Petros A. Tarantilis4Christos S. Pappas5Laboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceLaboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceLaboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceLaboratory of Quality and Safety of Agricultural Products, Landscape and Environment, Department of Agriculture, Hellenic Mediterranean University, Stavromenos, PC 71410 Heraklion, Crete, GreeceLaboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceLaboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceThis study aimed at an experimental design of response surface methodology (RSM) in the optimization of the dominant volatile fraction of Greek thyme honey using solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). For this purpose, a multiple response optimization was employed using desirability functions, which demand a search for optimal conditions for a set of responses simultaneously. A test set of eighty thyme honey samples were analyzed under the optimum conditions for validation of the proposed model. The optimized combination of isolation conditions was the temperature (60 °C), equilibration time (15 min), extraction time (30 min), magnetic stirrer speed (700 rpm), sample volume (6 mL), water: honey ratio (1:3 <i>v</i>/<i>w</i>) with total desirability over 0.50. It was found that the magnetic stirrer speed, which has not been evaluated before, had a positive effect, especially in combination with other factors. The above-developed methodology proved to be effective in the optimization of isolation of specific volatile compounds from a difficult matrix, like honey. This study could be a good basis for the development of novel RSM for other monofloral honey samples.https://www.mdpi.com/1420-3049/26/12/3612thyme honeyresponse surface methodologyoptimizationvolatilessolid-phase microextraction |
| spellingShingle | Marinos Xagoraris Alexandra Skouria Panagiota-Kyriaki Revelou Eleftherios Alissandrakis Petros A. Tarantilis Christos S. Pappas Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MS Molecules thyme honey response surface methodology optimization volatiles solid-phase microextraction |
| title | Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MS |
| title_full | Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MS |
| title_fullStr | Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MS |
| title_full_unstemmed | Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MS |
| title_short | Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MS |
| title_sort | response surface methodology to optimize the isolation of dominant volatile compounds from monofloral greek thyme honey using spme gc ms |
| topic | thyme honey response surface methodology optimization volatiles solid-phase microextraction |
| url | https://www.mdpi.com/1420-3049/26/12/3612 |
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