Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains
Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01557/full |
| _version_ | 1818050477210730496 |
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| author | Marcela Suárez-Esquivel Nazareth Ruiz-Villalobos Amanda Castillo-Zeledón César Jiménez-Rojas Martin R Roop II Diego J. Comerci Elías Barquero-Calvo Elías Barquero-Calvo Carlos Chacón-Díaz Clayton Caswell Kate S Baker Kate S Baker Esteban Chaves-Olarte Nicholas R Thomson Nicholas R Thomson Edgardo Moreno Edgardo Moreno Jean-Jacques Letesson Xavier De Bolle Caterina Guzmán-Verri Caterina Guzmán-Verri |
| author_facet | Marcela Suárez-Esquivel Nazareth Ruiz-Villalobos Amanda Castillo-Zeledón César Jiménez-Rojas Martin R Roop II Diego J. Comerci Elías Barquero-Calvo Elías Barquero-Calvo Carlos Chacón-Díaz Clayton Caswell Kate S Baker Kate S Baker Esteban Chaves-Olarte Nicholas R Thomson Nicholas R Thomson Edgardo Moreno Edgardo Moreno Jean-Jacques Letesson Xavier De Bolle Caterina Guzmán-Verri Caterina Guzmán-Verri |
| author_sort | Marcela Suárez-Esquivel |
| collection | DOAJ |
| description | Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing (WGS) analysis of the reference strain Brucella abortus 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version at www.wikipedia.Comparison of this genome with two publically available 2308 genomes showed significant differences, particularly indels related to insertional elements, suggesting variability related to the transposition of these elements within the same strain. Considering the outcome of high resolution genomic techniques in the bacteriology field, the conventional concept of strain definition needs to be revised. |
| first_indexed | 2024-12-10T10:54:06Z |
| format | Article |
| id | doaj.art-b1dbb67c7dbb493390c5435ef96d3d0c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T10:54:06Z |
| publishDate | 2016-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b1dbb67c7dbb493390c5435ef96d3d0c2022-12-22T01:51:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-09-01710.3389/fmicb.2016.01557221578Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strainsMarcela Suárez-Esquivel0Nazareth Ruiz-Villalobos1Amanda Castillo-Zeledón2César Jiménez-Rojas3Martin R Roop II4Diego J. Comerci5Elías Barquero-Calvo6Elías Barquero-Calvo7Carlos Chacón-Díaz8Clayton Caswell9Kate S Baker10Kate S Baker11Esteban Chaves-Olarte12Nicholas R Thomson13Nicholas R Thomson14Edgardo Moreno15Edgardo Moreno16Jean-Jacques Letesson17Xavier De Bolle18Caterina Guzmán-Verri19Caterina Guzmán-Verri20PIET, Escuela de Medicina Veterinaria, Universidad NacionalPIET, Escuela de Medicina Veterinaria, Universidad NacionalPIET, Escuela de Medicina Veterinaria, Universidad NacionalPIET, Escuela de Medicina Veterinaria, Universidad NacionalEast Carolina UniversityUniversidad Nacional de San MartínPIET, Escuela de Medicina Veterinaria, Universidad NacionalCentro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa RicaCentro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa RicaVirginia TechWellcome Trust Sanger InstituteUniversity of LiverpoolCentro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa RicaWellcome Trust Sanger InstituteThe London School of Hygiene and Tropical MedicinePIET, Escuela de Medicina Veterinaria, Universidad NacionalUniversidad de Costa RicaUniversité de NamurUniversité de NamurPIET, Escuela de Medicina Veterinaria, Universidad NacionalCentro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa RicaBrucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing (WGS) analysis of the reference strain Brucella abortus 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version at www.wikipedia.Comparison of this genome with two publically available 2308 genomes showed significant differences, particularly indels related to insertional elements, suggesting variability related to the transposition of these elements within the same strain. Considering the outcome of high resolution genomic techniques in the bacteriology field, the conventional concept of strain definition needs to be revised.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01557/fullBrucellaBrucella abortusWGSReference genomeReference strain |
| spellingShingle | Marcela Suárez-Esquivel Nazareth Ruiz-Villalobos Amanda Castillo-Zeledón César Jiménez-Rojas Martin R Roop II Diego J. Comerci Elías Barquero-Calvo Elías Barquero-Calvo Carlos Chacón-Díaz Clayton Caswell Kate S Baker Kate S Baker Esteban Chaves-Olarte Nicholas R Thomson Nicholas R Thomson Edgardo Moreno Edgardo Moreno Jean-Jacques Letesson Xavier De Bolle Caterina Guzmán-Verri Caterina Guzmán-Verri Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains Frontiers in Microbiology Brucella Brucella abortus WGS Reference genome Reference strain |
| title | Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains |
| title_full | Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains |
| title_fullStr | Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains |
| title_full_unstemmed | Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains |
| title_short | Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains |
| title_sort | brucella abortus strain 2308 wisconsin genome importance of the definition of reference strains |
| topic | Brucella Brucella abortus WGS Reference genome Reference strain |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01557/full |
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