Growth inhibition of Penicillium digitatum by antagonistic microorganisms isolated from various parts of orange tree
Penicillium digitatum (Pers.:Fr) Sacc. is the major cause of green mold disease in orange trees grown for orange production industry in Thailand. In this study, antagonistic microorganisms were isolated from various parts of healthy orange trees. A total of 121 microbial isolates were recovered from...
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| Format: | Article |
| Language: | English |
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Maejo University
2007-11-01
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| Series: | Maejo International Journal of Science and Technology |
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| Online Access: | http://www.mijst.mju.ac.th/vol1/208-215.pdf |
| _version_ | 1819094474809147392 |
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| author | Wasu Pathom-aree |
| author_facet | Wasu Pathom-aree |
| author_sort | Wasu Pathom-aree |
| collection | DOAJ |
| description | Penicillium digitatum (Pers.:Fr) Sacc. is the major cause of green mold disease in orange trees grown for orange production industry in Thailand. In this study, antagonistic microorganisms were isolated from various parts of healthy orange trees. A total of 121 microbial isolates were recovered from which 15 isolates showed inhibitory effect against P. digitatum by spot test technique. The inhibitory effect of these 15 isolates was also tested by agar disc diffusion method using culture filtrate from nutrient broth. It was found that isolate W1L1, D1L2 and W1L9 gave the best inhibition with a clear zone of 21, 15 and 14 mm in diameter, respectively. The effect of culture filtrate on spore germination was also investigated. The result showed that isolate W1L1 gave the highest inhibition after 8 hours of incubation with the lowest germination rate of 2.4%. Morphological and biochemical characterizations revealed that these isolates were members of the genus Bacillus and identified as B. pumilus. |
| first_indexed | 2024-12-21T23:27:59Z |
| format | Article |
| id | doaj.art-b1dfdbd7a786462e8336eb74dcb128d9 |
| institution | Directory Open Access Journal |
| issn | 1905-7873 |
| language | English |
| last_indexed | 2024-12-21T23:27:59Z |
| publishDate | 2007-11-01 |
| publisher | Maejo University |
| record_format | Article |
| series | Maejo International Journal of Science and Technology |
| spelling | doaj.art-b1dfdbd7a786462e8336eb74dcb128d92022-12-21T18:46:35ZengMaejo UniversityMaejo International Journal of Science and Technology1905-78732007-11-01102208215Growth inhibition of Penicillium digitatum by antagonistic microorganisms isolated from various parts of orange treeWasu Pathom-areePenicillium digitatum (Pers.:Fr) Sacc. is the major cause of green mold disease in orange trees grown for orange production industry in Thailand. In this study, antagonistic microorganisms were isolated from various parts of healthy orange trees. A total of 121 microbial isolates were recovered from which 15 isolates showed inhibitory effect against P. digitatum by spot test technique. The inhibitory effect of these 15 isolates was also tested by agar disc diffusion method using culture filtrate from nutrient broth. It was found that isolate W1L1, D1L2 and W1L9 gave the best inhibition with a clear zone of 21, 15 and 14 mm in diameter, respectively. The effect of culture filtrate on spore germination was also investigated. The result showed that isolate W1L1 gave the highest inhibition after 8 hours of incubation with the lowest germination rate of 2.4%. Morphological and biochemical characterizations revealed that these isolates were members of the genus Bacillus and identified as B. pumilus.http://www.mijst.mju.ac.th/vol1/208-215.pdfPenicillium digitatumantagonistic microorganismsorangesBacillusbiological control |
| spellingShingle | Wasu Pathom-aree Growth inhibition of Penicillium digitatum by antagonistic microorganisms isolated from various parts of orange tree Maejo International Journal of Science and Technology Penicillium digitatum antagonistic microorganisms oranges Bacillus biological control |
| title | Growth inhibition of Penicillium digitatum by antagonistic microorganisms isolated from various parts of orange tree |
| title_full | Growth inhibition of Penicillium digitatum by antagonistic microorganisms isolated from various parts of orange tree |
| title_fullStr | Growth inhibition of Penicillium digitatum by antagonistic microorganisms isolated from various parts of orange tree |
| title_full_unstemmed | Growth inhibition of Penicillium digitatum by antagonistic microorganisms isolated from various parts of orange tree |
| title_short | Growth inhibition of Penicillium digitatum by antagonistic microorganisms isolated from various parts of orange tree |
| title_sort | growth inhibition of penicillium digitatum by antagonistic microorganisms isolated from various parts of orange tree |
| topic | Penicillium digitatum antagonistic microorganisms oranges Bacillus biological control |
| url | http://www.mijst.mju.ac.th/vol1/208-215.pdf |
| work_keys_str_mv | AT wasupathomaree growthinhibitionofpenicilliumdigitatumbyantagonisticmicroorganismsisolatedfromvariouspartsoforangetree |