The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene
We describe the plastome of Jenufa minuta, a unicellular green alga whose precise relationship with the Sphaeropleales (Chlorophyceae) remains uncertain. This 206,680-bp genome contains 100 conserved genes, 11 of which exhibit 16 group I and seven group II introns. We discovered a trans-spliced grou...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-04-01
|
| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2020.1749165 |
| _version_ | 1797639235513090048 |
|---|---|
| author | Monique Turmel Antony T. Vincent Christian Otis Claude Lemieux |
| author_facet | Monique Turmel Antony T. Vincent Christian Otis Claude Lemieux |
| author_sort | Monique Turmel |
| collection | DOAJ |
| description | We describe the plastome of Jenufa minuta, a unicellular green alga whose precise relationship with the Sphaeropleales (Chlorophyceae) remains uncertain. This 206,680-bp genome contains 100 conserved genes, 11 of which exhibit 16 group I and seven group II introns. We discovered a trans-spliced group II intron in the rpl32 gene of J. minuta and its orthologs in all Sphaeropleales and several Chlamydomonadales; this is the first time this intron is documented. As in the mitochondria of J. minuta, the UGA codon is decoded as tryptophan. To our knowledge, this is the second report of a variant genetic code in plastids. |
| first_indexed | 2024-03-11T13:14:58Z |
| format | Article |
| id | doaj.art-b1e177441dc240bd9002d484c6c5638d |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:14:58Z |
| publishDate | 2020-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-b1e177441dc240bd9002d484c6c5638d2023-11-03T13:50:20ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592020-04-01521728173010.1080/23802359.2020.17491651749165The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 geneMonique Turmel0Antony T. Vincent1Christian Otis2Claude Lemieux3Institut de Biologie Intégrative et des Systèmes, Université LavalInstitut de Biologie Intégrative et des Systèmes, Université LavalInstitut de Biologie Intégrative et des Systèmes, Université LavalInstitut de Biologie Intégrative et des Systèmes, Université LavalWe describe the plastome of Jenufa minuta, a unicellular green alga whose precise relationship with the Sphaeropleales (Chlorophyceae) remains uncertain. This 206,680-bp genome contains 100 conserved genes, 11 of which exhibit 16 group I and seven group II introns. We discovered a trans-spliced group II intron in the rpl32 gene of J. minuta and its orthologs in all Sphaeropleales and several Chlamydomonadales; this is the first time this intron is documented. As in the mitochondria of J. minuta, the UGA codon is decoded as tryptophan. To our knowledge, this is the second report of a variant genetic code in plastids.http://dx.doi.org/10.1080/23802359.2020.1749165noncanonical genetic codesphaeroplealestrans-spliced group ii intronuga codon reassignment |
| spellingShingle | Monique Turmel Antony T. Vincent Christian Otis Claude Lemieux The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene Mitochondrial DNA. Part B. Resources noncanonical genetic code sphaeropleales trans-spliced group ii intron uga codon reassignment |
| title | The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene |
| title_full | The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene |
| title_fullStr | The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene |
| title_full_unstemmed | The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene |
| title_short | The complete plastome of the coccoid green alga Jenufa minuta (Chlorophyceae, incertae sedis) unveils a noncanonical genetic code and a previously unrecognized trans-spliced group II intron in the rpl32 gene |
| title_sort | complete plastome of the coccoid green alga jenufa minuta chlorophyceae incertae sedis unveils a noncanonical genetic code and a previously unrecognized trans spliced group ii intron in the rpl32 gene |
| topic | noncanonical genetic code sphaeropleales trans-spliced group ii intron uga codon reassignment |
| url | http://dx.doi.org/10.1080/23802359.2020.1749165 |
| work_keys_str_mv | AT moniqueturmel thecompleteplastomeofthecoccoidgreenalgajenufaminutachlorophyceaeincertaesedisunveilsanoncanonicalgeneticcodeandapreviouslyunrecognizedtranssplicedgroupiiintronintherpl32gene AT antonytvincent thecompleteplastomeofthecoccoidgreenalgajenufaminutachlorophyceaeincertaesedisunveilsanoncanonicalgeneticcodeandapreviouslyunrecognizedtranssplicedgroupiiintronintherpl32gene AT christianotis thecompleteplastomeofthecoccoidgreenalgajenufaminutachlorophyceaeincertaesedisunveilsanoncanonicalgeneticcodeandapreviouslyunrecognizedtranssplicedgroupiiintronintherpl32gene AT claudelemieux thecompleteplastomeofthecoccoidgreenalgajenufaminutachlorophyceaeincertaesedisunveilsanoncanonicalgeneticcodeandapreviouslyunrecognizedtranssplicedgroupiiintronintherpl32gene AT moniqueturmel completeplastomeofthecoccoidgreenalgajenufaminutachlorophyceaeincertaesedisunveilsanoncanonicalgeneticcodeandapreviouslyunrecognizedtranssplicedgroupiiintronintherpl32gene AT antonytvincent completeplastomeofthecoccoidgreenalgajenufaminutachlorophyceaeincertaesedisunveilsanoncanonicalgeneticcodeandapreviouslyunrecognizedtranssplicedgroupiiintronintherpl32gene AT christianotis completeplastomeofthecoccoidgreenalgajenufaminutachlorophyceaeincertaesedisunveilsanoncanonicalgeneticcodeandapreviouslyunrecognizedtranssplicedgroupiiintronintherpl32gene AT claudelemieux completeplastomeofthecoccoidgreenalgajenufaminutachlorophyceaeincertaesedisunveilsanoncanonicalgeneticcodeandapreviouslyunrecognizedtranssplicedgroupiiintronintherpl32gene |