Selective Tropism of Dengue Virus for Human Glycoprotein Ib
Abstract Since the hemorrhage in severe dengue seems to be primarily related to the defect of the platelet, the possibility that dengue virus (DENV) is selectively tropic for one of its surface receptors was investigated. Flow cytometric data of DENV-infected megakaryocytic cell line superficially e...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-20914-z |
| _version_ | 1818428258558935040 |
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| author | Nattapol Attatippaholkun Nont Kosaisawe Yaowalak U-Pratya Panthipa Supraditaporn Chanchao Lorthongpanich Kovit Pattanapanyasat Surapol Issaragrisil |
| author_facet | Nattapol Attatippaholkun Nont Kosaisawe Yaowalak U-Pratya Panthipa Supraditaporn Chanchao Lorthongpanich Kovit Pattanapanyasat Surapol Issaragrisil |
| author_sort | Nattapol Attatippaholkun |
| collection | DOAJ |
| description | Abstract Since the hemorrhage in severe dengue seems to be primarily related to the defect of the platelet, the possibility that dengue virus (DENV) is selectively tropic for one of its surface receptors was investigated. Flow cytometric data of DENV-infected megakaryocytic cell line superficially expressing human glycoprotein Ib (CD42b) and glycoprotein IIb/IIIa (CD41 and CD41a) were analyzed by our custom-written software in MATLAB. In two-dimensional analyses, intracellular DENV was detected in CD42b+, CD41+ and CD41a+ cells. In three-dimensional analyses, the DENV was exclusively detected in CD42b+ cells but not in CD42b− cells regardless of the other expressions. In single-cell virus-protein analyses, the amount of DENV was directly correlated with those of CD42b at the Pearson correlation coefficient of 0.9. Moreover, RT- PCR and apoptosis assays showed that DENV was able to replicate itself and release its new progeny from the infected CD42b+ cells and eventually killed those cells. These results provide evidence for the involvement of CD42b in DENV infection. |
| first_indexed | 2024-12-14T14:58:46Z |
| format | Article |
| id | doaj.art-b1e3089262e64129b89fc3fba2395319 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:58:46Z |
| publishDate | 2018-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b1e3089262e64129b89fc3fba23953192022-12-21T22:56:55ZengNature PortfolioScientific Reports2045-23222018-02-018111110.1038/s41598-018-20914-zSelective Tropism of Dengue Virus for Human Glycoprotein IbNattapol Attatippaholkun0Nont Kosaisawe1Yaowalak U-Pratya2Panthipa Supraditaporn3Chanchao Lorthongpanich4Kovit Pattanapanyasat5Surapol Issaragrisil6Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol UniversitySiriraj Laboratory for System Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol UniversitySiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol UniversitySiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol UniversitySiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol UniversitySiriraj Center of Excellence for Flow Cytometry, Faculty of Medicine Siriraj Hospital, Mahidol UniversitySiriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol UniversityAbstract Since the hemorrhage in severe dengue seems to be primarily related to the defect of the platelet, the possibility that dengue virus (DENV) is selectively tropic for one of its surface receptors was investigated. Flow cytometric data of DENV-infected megakaryocytic cell line superficially expressing human glycoprotein Ib (CD42b) and glycoprotein IIb/IIIa (CD41 and CD41a) were analyzed by our custom-written software in MATLAB. In two-dimensional analyses, intracellular DENV was detected in CD42b+, CD41+ and CD41a+ cells. In three-dimensional analyses, the DENV was exclusively detected in CD42b+ cells but not in CD42b− cells regardless of the other expressions. In single-cell virus-protein analyses, the amount of DENV was directly correlated with those of CD42b at the Pearson correlation coefficient of 0.9. Moreover, RT- PCR and apoptosis assays showed that DENV was able to replicate itself and release its new progeny from the infected CD42b+ cells and eventually killed those cells. These results provide evidence for the involvement of CD42b in DENV infection.https://doi.org/10.1038/s41598-018-20914-z |
| spellingShingle | Nattapol Attatippaholkun Nont Kosaisawe Yaowalak U-Pratya Panthipa Supraditaporn Chanchao Lorthongpanich Kovit Pattanapanyasat Surapol Issaragrisil Selective Tropism of Dengue Virus for Human Glycoprotein Ib Scientific Reports |
| title | Selective Tropism of Dengue Virus for Human Glycoprotein Ib |
| title_full | Selective Tropism of Dengue Virus for Human Glycoprotein Ib |
| title_fullStr | Selective Tropism of Dengue Virus for Human Glycoprotein Ib |
| title_full_unstemmed | Selective Tropism of Dengue Virus for Human Glycoprotein Ib |
| title_short | Selective Tropism of Dengue Virus for Human Glycoprotein Ib |
| title_sort | selective tropism of dengue virus for human glycoprotein ib |
| url | https://doi.org/10.1038/s41598-018-20914-z |
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