Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle
Polyelectrolytes assembled layer-by-layer (PEMs) are commonly used as functional coatings to build-up biological interfaces, particularly suitable as compatible layers for the interaction with a biological medium, providing suitable conditions to promote or prevent cell seeding while maintaining the...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/13/2643 |
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| author | Anna Alba Giusy Villaggio Grazia Maria Lucia Messina Massimo Caruso Concetta Federico Maria Teresa Cambria Giovanni Marletta Fulvia Sinatra |
| author_facet | Anna Alba Giusy Villaggio Grazia Maria Lucia Messina Massimo Caruso Concetta Federico Maria Teresa Cambria Giovanni Marletta Fulvia Sinatra |
| author_sort | Anna Alba |
| collection | DOAJ |
| description | Polyelectrolytes assembled layer-by-layer (PEMs) are commonly used as functional coatings to build-up biological interfaces, particularly suitable as compatible layers for the interaction with a biological medium, providing suitable conditions to promote or prevent cell seeding while maintaining the phenotype. The proper assessment of the biocompatibility of PEMs and the elucidation of the related mechanisms are therefore of paramount importance. In this study, we report in detail the effect of two different PEM endings, polystyrene sulfonate (PSS) and polyethylenimine (PEI), respectively, on the cell adhesion, growth, and viability of human bone mesenchymal stromal cells (MSCs). The results have shown that PSS-ended substrates appear to be the most suitable to drive the cell adhesion and phenotype maintenance of MSCs, showing good biocompatibility. On the contrary, while the cells seem to adhere more quickly and strongly on the PEI-ended surfaces, the interaction with PEI significantly affects the growth and viability, reducing the cell spreading capability, by sequestering the adhesion molecules already in the very early steps of cell–substrate contact. These results point to the promotion of a cytostatic effect of PEI, rather than the often-claimed cytotoxicity. |
| first_indexed | 2024-03-09T03:57:48Z |
| format | Article |
| id | doaj.art-b1e402ddc39841d6aa12e20338d51717 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T03:57:48Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b1e402ddc39841d6aa12e20338d517172023-12-03T14:18:47ZengMDPI AGPolymers2073-43602022-06-011413264310.3390/polym14132643Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell CycleAnna Alba0Giusy Villaggio1Grazia Maria Lucia Messina2Massimo Caruso3Concetta Federico4Maria Teresa Cambria5Giovanni Marletta6Fulvia Sinatra7Section of Biology and Genetic, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia, 65, 95123 Catania, ItalySection of Biology and Genetic, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia, 65, 95123 Catania, ItalyLaboratory for Molecular Surface and Nanotechnology (LAMSUN), Department of Chemical Sciences, University of Catania and CSGI, Viale A. Doria, 6, 95125 Catania, ItalySection of Biochemistry, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia, 65, 95123 Catania, ItalySection of Animal Biology, Department of Biological, Geological and Environmental Sciences, University of Catania, Via Androne, 81, 95124 Catania, ItalySection of Biochemistry, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia, 65, 95123 Catania, ItalyLaboratory for Molecular Surface and Nanotechnology (LAMSUN), Department of Chemical Sciences, University of Catania and CSGI, Viale A. Doria, 6, 95125 Catania, ItalySection of Biology and Genetic, Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia, 65, 95123 Catania, ItalyPolyelectrolytes assembled layer-by-layer (PEMs) are commonly used as functional coatings to build-up biological interfaces, particularly suitable as compatible layers for the interaction with a biological medium, providing suitable conditions to promote or prevent cell seeding while maintaining the phenotype. The proper assessment of the biocompatibility of PEMs and the elucidation of the related mechanisms are therefore of paramount importance. In this study, we report in detail the effect of two different PEM endings, polystyrene sulfonate (PSS) and polyethylenimine (PEI), respectively, on the cell adhesion, growth, and viability of human bone mesenchymal stromal cells (MSCs). The results have shown that PSS-ended substrates appear to be the most suitable to drive the cell adhesion and phenotype maintenance of MSCs, showing good biocompatibility. On the contrary, while the cells seem to adhere more quickly and strongly on the PEI-ended surfaces, the interaction with PEI significantly affects the growth and viability, reducing the cell spreading capability, by sequestering the adhesion molecules already in the very early steps of cell–substrate contact. These results point to the promotion of a cytostatic effect of PEI, rather than the often-claimed cytotoxicity.https://www.mdpi.com/2073-4360/14/13/2643polymeric polyelectrolyte multilayersbone mesenchymal stromal cellsQCM-Dcell spreadingcytostasis |
| spellingShingle | Anna Alba Giusy Villaggio Grazia Maria Lucia Messina Massimo Caruso Concetta Federico Maria Teresa Cambria Giovanni Marletta Fulvia Sinatra Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle Polymers polymeric polyelectrolyte multilayers bone mesenchymal stromal cells QCM-D cell spreading cytostasis |
| title | Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle |
| title_full | Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle |
| title_fullStr | Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle |
| title_full_unstemmed | Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle |
| title_short | Cytostatic Effects of Polyethyleneimine Surfaces on the Mesenchymal Stromal Cell Cycle |
| title_sort | cytostatic effects of polyethyleneimine surfaces on the mesenchymal stromal cell cycle |
| topic | polymeric polyelectrolyte multilayers bone mesenchymal stromal cells QCM-D cell spreading cytostasis |
| url | https://www.mdpi.com/2073-4360/14/13/2643 |
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