A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme
Plants are not only challenged by pathogenic organisms but also colonized by commensal microbes. The network of interactions these microbes establish with their host and among each other is suggested to contribute to the immune responses of plants against pathogens. In wild Arabidopsis thaliana popu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/65306 |
| _version_ | 1811236078243282944 |
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| author | Katharina Eitzen Priyamedha Sengupta Samuel Kroll Eric Kemen Gunther Doehlemann |
| author_facet | Katharina Eitzen Priyamedha Sengupta Samuel Kroll Eric Kemen Gunther Doehlemann |
| author_sort | Katharina Eitzen |
| collection | DOAJ |
| description | Plants are not only challenged by pathogenic organisms but also colonized by commensal microbes. The network of interactions these microbes establish with their host and among each other is suggested to contribute to the immune responses of plants against pathogens. In wild Arabidopsis thaliana populations, the oomycete pathogen Albugo laibachii plays an influential role in structuring the leaf phyllosphere. We show that the epiphytic yeast Moesziomyces bullatus ex Albugo on Arabidopsis, a close relative of pathogenic smut fungi, is an antagonistic member of the A. thaliana phyllosphere, which reduces infection of A. thaliana by A. laibachii. Combination of transcriptomics, reverse genetics, and protein characterization identified a GH25 hydrolase with lysozyme activity as a major effector of this microbial antagonism. Our findings broaden the understanding of microbial interactions within the phyllosphere, provide insights into the evolution of epiphytic basidiomycete yeasts, and pave the way for novel biocontrol strategies. |
| first_indexed | 2024-04-12T12:02:48Z |
| format | Article |
| id | doaj.art-b1e4c873b9b249b9966f3390bad1eaf8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:02:48Z |
| publishDate | 2021-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-b1e4c873b9b249b9966f3390bad1eaf82022-12-22T03:33:48ZengeLife Sciences Publications LtdeLife2050-084X2021-01-011010.7554/eLife.65306A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozymeKatharina Eitzen0Priyamedha Sengupta1Samuel Kroll2Eric Kemen3Gunther Doehlemann4https://orcid.org/0000-0002-7353-8456Institute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Center for Molecular Biosciences, Cologne, Germany; Max Planck Institute for Plant Breeding Research, Cologne, GermanyInstitute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Center for Molecular Biosciences, Cologne, GermanyMax Planck Institute for Plant Breeding Research, Cologne, GermanyMax Planck Institute for Plant Breeding Research, Cologne, Germany; Department of Microbial Interactions, IMIT/ZMBP, University of Tübingen, Tübingen, GermanyInstitute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Center for Molecular Biosciences, Cologne, GermanyPlants are not only challenged by pathogenic organisms but also colonized by commensal microbes. The network of interactions these microbes establish with their host and among each other is suggested to contribute to the immune responses of plants against pathogens. In wild Arabidopsis thaliana populations, the oomycete pathogen Albugo laibachii plays an influential role in structuring the leaf phyllosphere. We show that the epiphytic yeast Moesziomyces bullatus ex Albugo on Arabidopsis, a close relative of pathogenic smut fungi, is an antagonistic member of the A. thaliana phyllosphere, which reduces infection of A. thaliana by A. laibachii. Combination of transcriptomics, reverse genetics, and protein characterization identified a GH25 hydrolase with lysozyme activity as a major effector of this microbial antagonism. Our findings broaden the understanding of microbial interactions within the phyllosphere, provide insights into the evolution of epiphytic basidiomycete yeasts, and pave the way for novel biocontrol strategies.https://elifesciences.org/articles/65306microbial antagonismalbugo laibachiiustilaginalesarabidopsistranscriptomicseffectors |
| spellingShingle | Katharina Eitzen Priyamedha Sengupta Samuel Kroll Eric Kemen Gunther Doehlemann A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme eLife microbial antagonism albugo laibachii ustilaginales arabidopsis transcriptomics effectors |
| title | A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme |
| title_full | A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme |
| title_fullStr | A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme |
| title_full_unstemmed | A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme |
| title_short | A fungal member of the Arabidopsis thaliana phyllosphere antagonizes Albugo laibachii via a GH25 lysozyme |
| title_sort | fungal member of the arabidopsis thaliana phyllosphere antagonizes albugo laibachii via a gh25 lysozyme |
| topic | microbial antagonism albugo laibachii ustilaginales arabidopsis transcriptomics effectors |
| url | https://elifesciences.org/articles/65306 |
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