Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection
A headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME/GC-MS) method was used to study the volatile organic compounds (VOCs) associated with the differential immune response of tomato plants infected with the recombinant strain of potato virus Y (PVYC-to), necrogenic t...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923000448 |
| _version_ | 1797744083598311424 |
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| author | Roberta SPANÒ Mariarosaria MASTROCHIRICO Francesco LONGOBARDI Salvatore CERVELLIERI Vincenzo LIPPOLIS Tiziana MASCIA |
| author_facet | Roberta SPANÒ Mariarosaria MASTROCHIRICO Francesco LONGOBARDI Salvatore CERVELLIERI Vincenzo LIPPOLIS Tiziana MASCIA |
| author_sort | Roberta SPANÒ |
| collection | DOAJ |
| description | A headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME/GC-MS) method was used to study the volatile organic compounds (VOCs) associated with the differential immune response of tomato plants infected with the recombinant strain of potato virus Y (PVYC-to), necrogenic to tomato. Analysis was carried out in UC82 (UC), a virus susceptible tomato variety, comparing the same UC plants grafted or not onto a virus tolerant tomato ecotype, Manduria (Ma); the three types of samples used for the GC-MS analysis were mock-inoculated UC/Ma plants, UC/Ma+PVYC-to and UC+PVYC-to plants; the VOCs obtained were 111. Results from symptomatic PVYC-to-infected UC plants showed a VOCs composition enriched in alcohols, fatty acid derivates, benzenoids, and salicylic acid derivatives, while in mock-inoculated UC/Ma plants VOCs were mainly characterized by methyl ester compounds. The VOC profile was in line with RNAseq data analyses, denoting that PVYC-to viral RNA accumulation and disease symptoms induce the specific transcriptional activation of genes involved in VOCs biosynthesis. Furthermore, principal component analysis highlighted that VOCs of PVYC-to-infected and mock-inoculated grafted plants were much closer each other than that of symptomatic PVYC-to-infected non-grafted UC plants. These results suggest that VOCs profiles of tomato plants are related to the viral RNA accumulation, disease intensity and graft-derived tolerance to PVYC-to infection. |
| first_indexed | 2024-03-12T15:04:39Z |
| format | Article |
| id | doaj.art-b1e5599f17ff483584e2a78702613a0d |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-12T15:04:39Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-b1e5599f17ff483584e2a78702613a0d2023-08-13T04:53:34ZengElsevierJournal of Integrative Agriculture2095-31192023-08-0122824262440Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infectionRoberta SPANÒ0Mariarosaria MASTROCHIRICO1Francesco LONGOBARDI2Salvatore CERVELLIERI3Vincenzo LIPPOLIS4Tiziana MASCIA5Department of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, Via Amendola 165/A, Bari 70126, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, Via Amendola 165/A, Bari 70126, ItalyDepartment of Chemistry, University of Bari “Aldo Moro”, Via Orabona 4, Bari 70126, Italy; Correspondence Francesco LONGOBARDI, Tel: +39-0805442042National Research Council (CNR), Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, Bari 70126, ItalyNational Research Council (CNR), Institute of Sciences of Food Production (ISPA), Via Amendola 122/O, Bari 70126, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, Via Amendola 165/A, Bari 70126, Italy; Correspondence Tiziana MASCIA, Tel: +39-0805442913A headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME/GC-MS) method was used to study the volatile organic compounds (VOCs) associated with the differential immune response of tomato plants infected with the recombinant strain of potato virus Y (PVYC-to), necrogenic to tomato. Analysis was carried out in UC82 (UC), a virus susceptible tomato variety, comparing the same UC plants grafted or not onto a virus tolerant tomato ecotype, Manduria (Ma); the three types of samples used for the GC-MS analysis were mock-inoculated UC/Ma plants, UC/Ma+PVYC-to and UC+PVYC-to plants; the VOCs obtained were 111. Results from symptomatic PVYC-to-infected UC plants showed a VOCs composition enriched in alcohols, fatty acid derivates, benzenoids, and salicylic acid derivatives, while in mock-inoculated UC/Ma plants VOCs were mainly characterized by methyl ester compounds. The VOC profile was in line with RNAseq data analyses, denoting that PVYC-to viral RNA accumulation and disease symptoms induce the specific transcriptional activation of genes involved in VOCs biosynthesis. Furthermore, principal component analysis highlighted that VOCs of PVYC-to-infected and mock-inoculated grafted plants were much closer each other than that of symptomatic PVYC-to-infected non-grafted UC plants. These results suggest that VOCs profiles of tomato plants are related to the viral RNA accumulation, disease intensity and graft-derived tolerance to PVYC-to infection.http://www.sciencedirect.com/science/article/pii/S2095311923000448tomatopotyvirusVOCsdefensegrafted plants |
| spellingShingle | Roberta SPANÒ Mariarosaria MASTROCHIRICO Francesco LONGOBARDI Salvatore CERVELLIERI Vincenzo LIPPOLIS Tiziana MASCIA Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection Journal of Integrative Agriculture tomato potyvirus VOCs defense grafted plants |
| title | Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection |
| title_full | Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection |
| title_fullStr | Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection |
| title_full_unstemmed | Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection |
| title_short | Characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection |
| title_sort | characterization of volatile organic compounds in grafted tomato plants upon potyvirus necrotic infection |
| topic | tomato potyvirus VOCs defense grafted plants |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923000448 |
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