Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery
The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH4+) regulate its growth is not well understood. The current study focused on the effects of NH4+ on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH4+ deprivation a...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.963269/full |
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| author | Yu Wang Jia-Xue Ouyang Dong-Mei Fan Shu-Mao Wang Yi-Min Xuan Xiao-Chang Wang Xiao-Chang Wang Xin-Qiang Zheng |
| author_facet | Yu Wang Jia-Xue Ouyang Dong-Mei Fan Shu-Mao Wang Yi-Min Xuan Xiao-Chang Wang Xiao-Chang Wang Xin-Qiang Zheng |
| author_sort | Yu Wang |
| collection | DOAJ |
| description | The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH4+) regulate its growth is not well understood. The current study focused on the effects of NH4+ on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH4+ deprivation and resupply in tea plants shoots. Through short- and long-term NH4+ deficiency, the dynamic response to NH4+ stress was investigated. The most significant effects of NH4+ deficiency were found to be on photosynthesis and gene ontology (GO) enrichment varied with the length of NH4+ deprivation. Enriched KEGG pathways were also different when NH4+ was resupplied at different concentrations which may indicate reasons for tolerance of high NH4+ concentration. Using weighted gene co-expression network analysis (WGCNA), modules related to significant tea components, tea polyphenols and free amino acids, were identified. Hence, NH4+ could be regarded as a signaling molecule with the response of catechins shown to be higher than that of amino acids. The current work represents a comprehensive transcriptomic analysis of plant responses to NH4+ and reveals many potential genes regulated by NH4+ in tea plants. Such findings may lead to improvements in nitrogen efficiency of tea plants. |
| first_indexed | 2024-04-11T11:08:49Z |
| format | Article |
| id | doaj.art-b1e794327c3f4411b4e600aed8c739e6 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T11:08:49Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b1e794327c3f4411b4e600aed8c739e62022-12-22T04:28:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-08-011310.3389/fpls.2022.963269963269Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recoveryYu Wang0Jia-Xue Ouyang1Dong-Mei Fan2Shu-Mao Wang3Yi-Min Xuan4Xiao-Chang Wang5Xiao-Chang Wang6Xin-Qiang Zheng7College of Agriculture and Biotechnology, Tea Research Institute, Zhejiang University, Hangzhou, ChinaCollege of Agriculture and Biotechnology, Tea Research Institute, Zhejiang University, Hangzhou, ChinaCollege of Agriculture and Biotechnology, Tea Research Institute, Zhejiang University, Hangzhou, ChinaCollege of Agriculture and Biotechnology, Tea Research Institute, Zhejiang University, Hangzhou, ChinaCollege of Agriculture and Biotechnology, Tea Research Institute, Zhejiang University, Hangzhou, ChinaCollege of Agriculture and Biotechnology, Tea Research Institute, Zhejiang University, Hangzhou, ChinaInstitute of Dafo Longjing, Xinchang, ChinaCollege of Agriculture and Biotechnology, Tea Research Institute, Zhejiang University, Hangzhou, ChinaThe tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH4+) regulate its growth is not well understood. The current study focused on the effects of NH4+ on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH4+ deprivation and resupply in tea plants shoots. Through short- and long-term NH4+ deficiency, the dynamic response to NH4+ stress was investigated. The most significant effects of NH4+ deficiency were found to be on photosynthesis and gene ontology (GO) enrichment varied with the length of NH4+ deprivation. Enriched KEGG pathways were also different when NH4+ was resupplied at different concentrations which may indicate reasons for tolerance of high NH4+ concentration. Using weighted gene co-expression network analysis (WGCNA), modules related to significant tea components, tea polyphenols and free amino acids, were identified. Hence, NH4+ could be regarded as a signaling molecule with the response of catechins shown to be higher than that of amino acids. The current work represents a comprehensive transcriptomic analysis of plant responses to NH4+ and reveals many potential genes regulated by NH4+ in tea plants. Such findings may lead to improvements in nitrogen efficiency of tea plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.963269/fulltranscriptomeammoniumtea plantsRNA-sequencingnitrogenWGCNA |
| spellingShingle | Yu Wang Jia-Xue Ouyang Dong-Mei Fan Shu-Mao Wang Yi-Min Xuan Xiao-Chang Wang Xiao-Chang Wang Xin-Qiang Zheng Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery Frontiers in Plant Science transcriptome ammonium tea plants RNA-sequencing nitrogen WGCNA |
| title | Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery |
| title_full | Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery |
| title_fullStr | Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery |
| title_full_unstemmed | Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery |
| title_short | Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery |
| title_sort | transcriptome analysis of tea camellia sinensis leaves in response to ammonium starvation and recovery |
| topic | transcriptome ammonium tea plants RNA-sequencing nitrogen WGCNA |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.963269/full |
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