Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome
In human nutrition, bovine milk is an essential source of bioavailable vitamin B12 and B12-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B12 and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectiv...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-05-01
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| Series: | JDS Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266691022100034X |
| _version_ | 1797788479230312448 |
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| author | Grum Gebreyesus Nina Aagaard Poulsen Mette Krogh Larsen Lotte Bach Larsen Esben Skipper Sørensen Christian Würtz Heegaard Bart Buitenhuis |
| author_facet | Grum Gebreyesus Nina Aagaard Poulsen Mette Krogh Larsen Lotte Bach Larsen Esben Skipper Sørensen Christian Würtz Heegaard Bart Buitenhuis |
| author_sort | Grum Gebreyesus |
| collection | DOAJ |
| description | In human nutrition, bovine milk is an essential source of bioavailable vitamin B12 and B12-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B12 and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectively. Additionally, we conducted whole-genome association analysis to identify SNP and genes associated with vitamin B12 and transcobalamin. Our results indicated moderate to high heritability for vitamin B12 (0.37 ± 0.18) and transcobalamin (0.61 ± 0.13) content in the Danish Holstein. With a significance threshold of –log10 P-value > 5.87, significant associations were detected between SNP in Bos taurus autosome (BTA)17 and the log-transformed transcobalamin content of milk; no significant association was detected for vitamin B12. The significant region in BTA17 was imputed to full sequence for further fine mapping, and the SNP with the most significant associations to transcobalamin were assigned to the transcobalamin 2 (TCN2) gene. |
| first_indexed | 2024-03-13T01:36:07Z |
| format | Article |
| id | doaj.art-b1e7b056554b47e7807ad5ea337f87f8 |
| institution | Directory Open Access Journal |
| issn | 2666-9102 |
| language | English |
| last_indexed | 2024-03-13T01:36:07Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JDS Communications |
| spelling | doaj.art-b1e7b056554b47e7807ad5ea337f87f82023-07-04T05:10:31ZengElsevierJDS Communications2666-91022021-05-0123127131Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genomeGrum Gebreyesus0Nina Aagaard Poulsen1Mette Krogh Larsen2Lotte Bach Larsen3Esben Skipper Sørensen4Christian Würtz Heegaard5Bart Buitenhuis6Center for Quantitative Genetics and Genomics, Aarhus University, Blichers Allé 20, PO Box 50, DK-8830 Tjele, Denmark; Corresponding authorDepartment of Food Science, Aarhus University, Agro Food Park 48, DK-8200 Aarhus N, DenmarkDepartment of Food Science, Aarhus University, Agro Food Park 48, DK-8200 Aarhus N, Denmark; Arla Foods Amba, Mæalkevejen 4, DK-6920 Videbæk, DenmarkDepartment of Food Science, Aarhus University, Agro Food Park 48, DK-8200 Aarhus N, DenmarkMolecular Nutrition, Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, DenmarkMolecular Nutrition, Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, DK-8000 Aarhus C, DenmarkCenter for Quantitative Genetics and Genomics, Aarhus University, Blichers Allé 20, PO Box 50, DK-8830 Tjele, DenmarkIn human nutrition, bovine milk is an essential source of bioavailable vitamin B12 and B12-binding proteins, including transcobalamin. In this study, we estimated genetic parameters for milk content of vitamin B12 and transcobalamin using milk samples from 341 and 663 Danish Holstein cows, respectively. Additionally, we conducted whole-genome association analysis to identify SNP and genes associated with vitamin B12 and transcobalamin. Our results indicated moderate to high heritability for vitamin B12 (0.37 ± 0.18) and transcobalamin (0.61 ± 0.13) content in the Danish Holstein. With a significance threshold of –log10 P-value > 5.87, significant associations were detected between SNP in Bos taurus autosome (BTA)17 and the log-transformed transcobalamin content of milk; no significant association was detected for vitamin B12. The significant region in BTA17 was imputed to full sequence for further fine mapping, and the SNP with the most significant associations to transcobalamin were assigned to the transcobalamin 2 (TCN2) gene.http://www.sciencedirect.com/science/article/pii/S266691022100034X |
| spellingShingle | Grum Gebreyesus Nina Aagaard Poulsen Mette Krogh Larsen Lotte Bach Larsen Esben Skipper Sørensen Christian Würtz Heegaard Bart Buitenhuis Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome JDS Communications |
| title | Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome |
| title_full | Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome |
| title_fullStr | Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome |
| title_full_unstemmed | Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome |
| title_short | Vitamin B12 and transcobalamin in bovine milk: Genetic variation and genome-wide association with loci along the genome |
| title_sort | vitamin b12 and transcobalamin in bovine milk genetic variation and genome wide association with loci along the genome |
| url | http://www.sciencedirect.com/science/article/pii/S266691022100034X |
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