Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines
Introduction and objective Since antiquity, C. babylonica (L.) L. extracts has been used as a remedy for primary health care in traditional medicine. In this study, a total of seven different crude extracts (acetone, chloroform, hexane, ethylacetate, methanol, ethanol and water) from branches and...
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| Format: | Article |
| Language: | English |
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Institute of Rural Health
2019-06-01
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| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/aaem/Antimicrobial-property-and-antiproliferative-activity-of-Centaurea-babylonica-L-L,108563,0,2.html |
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| author | Nur Ceyhan Güvensen Dilek Keskin Hatice Güneş Müjgan Kesik Oktay Hasan Yıldırım |
| author_facet | Nur Ceyhan Güvensen Dilek Keskin Hatice Güneş Müjgan Kesik Oktay Hasan Yıldırım |
| author_sort | Nur Ceyhan Güvensen |
| collection | DOAJ |
| description | Introduction and objective
Since antiquity, C. babylonica (L.) L. extracts has been used as a remedy for primary health care in traditional medicine. In this study, a total of seven different crude extracts (acetone, chloroform, hexane, ethylacetate, methanol, ethanol and water) from branches and leaves of C. babylonica (L.) L. were prepared to determine antimicrobial and antiproliferative activity against cancer cell lines.
Material and Methods
MIC assay was used for antimicrobial activity against gram positive and gram negative bacteria,
and one yeast. MTT assay was applied to screen the antiproliferative activity of seven extracts, and to determine dose- and time-dependent effects of the aceton extract on A549, PC-3, MCF-7, and HeLa cell lines.
Results
The aceton extract of C.babylonica (L.) L. showed the best antibacterial activity against Bacillus cereus, P. aeruginosa and C. albicans (MIC: 1.6 mg/mL). GC-MS analyses allowed six compounds to be determined; the main constituents of acetone extract from C. babylonica (L.) L. were diacetone alcohol (53.47 %), 1-dexadecene (10.19 %) and 1-tetradecene (8.67 %). In addition, seven different solvent extracts at 500 µg/mL caused antiproliferative activity between 84% – 88%, compared to control. Dose-dependent effects of the extracts on A549 cells indicated that chloroform, ethyl acetate, and aceton extract were the most effective extracts with the IC 50 values of 9, 33, and 36 µg/mL, respectively.
Conclusions
The results clearly demonstrate that C. babylonica (L.) L. exhibited a strong antimicrobial effect and
antiproliferative activity against cancer cells in vitro. Further studies are required to isolate and characterize the active pure compounds responsible for the antimicrobial and antiproliferative activities. |
| first_indexed | 2024-12-13T06:44:43Z |
| format | Article |
| id | doaj.art-b1e7e0c724ad492d83d12241f94abcee |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-13T06:44:43Z |
| publishDate | 2019-06-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-b1e7e0c724ad492d83d12241f94abcee2022-12-21T23:56:19ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632019-06-0126229029710.26444/aaem/108563108563Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell linesNur Ceyhan Güvensen0Dilek Keskin1Hatice Güneş2Müjgan Kesik Oktay3Hasan Yıldırım4Muğla Sıtkı Koçman University, Mugla, TurkeyAdanan Menderes University, Aydin, TurkeyMuğla Sıtkı Koçman University, Mugla, TurkeyMuğla Sıtkı Koçman University, Mugla, TurkeyEge University, Izmar, TurkeyIntroduction and objective Since antiquity, C. babylonica (L.) L. extracts has been used as a remedy for primary health care in traditional medicine. In this study, a total of seven different crude extracts (acetone, chloroform, hexane, ethylacetate, methanol, ethanol and water) from branches and leaves of C. babylonica (L.) L. were prepared to determine antimicrobial and antiproliferative activity against cancer cell lines. Material and Methods MIC assay was used for antimicrobial activity against gram positive and gram negative bacteria, and one yeast. MTT assay was applied to screen the antiproliferative activity of seven extracts, and to determine dose- and time-dependent effects of the aceton extract on A549, PC-3, MCF-7, and HeLa cell lines. Results The aceton extract of C.babylonica (L.) L. showed the best antibacterial activity against Bacillus cereus, P. aeruginosa and C. albicans (MIC: 1.6 mg/mL). GC-MS analyses allowed six compounds to be determined; the main constituents of acetone extract from C. babylonica (L.) L. were diacetone alcohol (53.47 %), 1-dexadecene (10.19 %) and 1-tetradecene (8.67 %). In addition, seven different solvent extracts at 500 µg/mL caused antiproliferative activity between 84% – 88%, compared to control. Dose-dependent effects of the extracts on A549 cells indicated that chloroform, ethyl acetate, and aceton extract were the most effective extracts with the IC 50 values of 9, 33, and 36 µg/mL, respectively. Conclusions The results clearly demonstrate that C. babylonica (L.) L. exhibited a strong antimicrobial effect and antiproliferative activity against cancer cells in vitro. Further studies are required to isolate and characterize the active pure compounds responsible for the antimicrobial and antiproliferative activities.http://www.journalssystem.com/aaem/Antimicrobial-property-and-antiproliferative-activity-of-Centaurea-babylonica-L-L,108563,0,2.htmlCentaura babylonicaantimicrobial activityantiproliferative activityA-549PC-3MCF-7 and HeLa cell lineschemical compositionGC-MS |
| spellingShingle | Nur Ceyhan Güvensen Dilek Keskin Hatice Güneş Müjgan Kesik Oktay Hasan Yıldırım Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines Annals of Agricultural and Environmental Medicine Centaura babylonica antimicrobial activity antiproliferative activity A-549 PC-3 MCF-7 and HeLa cell lines chemical composition GC-MS |
| title | Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines |
| title_full | Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines |
| title_fullStr | Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines |
| title_full_unstemmed | Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines |
| title_short | Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines |
| title_sort | antimicrobial property and antiproliferative activity of centaurea babylonica l l on human carcinomas and cervical cancer cell lines |
| topic | Centaura babylonica antimicrobial activity antiproliferative activity A-549 PC-3 MCF-7 and HeLa cell lines chemical composition GC-MS |
| url | http://www.journalssystem.com/aaem/Antimicrobial-property-and-antiproliferative-activity-of-Centaurea-babylonica-L-L,108563,0,2.html |
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