Detection of <i>GSTM1</i>-null Genotype in Women Undergoing IVF Treatment
Background: Glutathione S-transferase (GST) M1 belongs to a family of detoxification enzymes and deficiency in enzyme activity is due to a homozygous deletion of the <i>GSTM1</i> gene. Several studies reveal a possible correlation between female infertility and <i>GSTM1</i> p...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/12/23/7269 |
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| author | Ioannis Chrysanthopoulos Angelos Petsavas Despoina Mavrogianni Anastasios Potiris Nikolaos Machairiotis Eirini Drakaki Dionysios Vrachnis Pavlos Machairoudias Theodoros Karampitsakos Paraskevas Perros Christos Koratzanis Peter Drakakis Sofoklis Stavros |
| author_facet | Ioannis Chrysanthopoulos Angelos Petsavas Despoina Mavrogianni Anastasios Potiris Nikolaos Machairiotis Eirini Drakaki Dionysios Vrachnis Pavlos Machairoudias Theodoros Karampitsakos Paraskevas Perros Christos Koratzanis Peter Drakakis Sofoklis Stavros |
| author_sort | Ioannis Chrysanthopoulos |
| collection | DOAJ |
| description | Background: Glutathione S-transferase (GST) M1 belongs to a family of detoxification enzymes and deficiency in enzyme activity is due to a homozygous deletion of the <i>GSTM1</i> gene. Several studies reveal a possible correlation between female infertility and <i>GSTM1</i> polymorphisms. The aim of this study is to investigate the effect of the <i>GSTM1</i>-null polymorphism in female infertility as well as in IVF parameters. Methods: In the study group 125 women were classified as infertile according to WHO and 49 women with at least one successful pregnancy and no miscarriages, as control group. Genomic DNA from blood samples was isolated and PCR amplification was applied to determine the presence of <i>GSTM1</i>-null genotype. Results: Data analysis demonstrated a statistically significant higher presence of <i>GSTM1</i>-null variant in the infertile group compared to the control group. In a subgroup analysis of the infertile group, the estradiol levels, the number of fertilized oocytes as well as the number and the quality of the cumulus-oocyte complex, were statistically significant higher in women detected with the wildtype of <i>GSTM1</i> gene compared to those who had the <i>GSTM1</i> null genotype (deletion). Conclusions: Our study results propose a possible involvement of GMST1 in female infertility and may help elucidate possible interactions between the microenvironment of oocytes and the oxidative stress. |
| first_indexed | 2024-03-09T01:49:11Z |
| format | Article |
| id | doaj.art-b1ea5503fd5e4948a690b7a5ba30d734 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T01:49:11Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-b1ea5503fd5e4948a690b7a5ba30d7342023-12-08T15:19:02ZengMDPI AGJournal of Clinical Medicine2077-03832023-11-011223726910.3390/jcm12237269Detection of <i>GSTM1</i>-null Genotype in Women Undergoing IVF TreatmentIoannis Chrysanthopoulos0Angelos Petsavas1Despoina Mavrogianni2Anastasios Potiris3Nikolaos Machairiotis4Eirini Drakaki5Dionysios Vrachnis6Pavlos Machairoudias7Theodoros Karampitsakos8Paraskevas Perros9Christos Koratzanis10Peter Drakakis11Sofoklis Stavros12First Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceThird Department of Obstetrics and Gynecology, University General Hospital “ATTIKON”, Medical School, National and Kapodistrian University of Athens, 124 62 Athens, GreeceThird Department of Obstetrics and Gynecology, University General Hospital “ATTIKON”, Medical School, National and Kapodistrian University of Athens, 124 62 Athens, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceMedical School, National and Kapodistrian University of Athens, 115 27 Athens, GreeceThird Department of Obstetrics and Gynecology, University General Hospital “ATTIKON”, Medical School, National and Kapodistrian University of Athens, 124 62 Athens, GreeceThird Department of Obstetrics and Gynecology, University General Hospital “ATTIKON”, Medical School, National and Kapodistrian University of Athens, 124 62 Athens, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceThird Department of Obstetrics and Gynecology, University General Hospital “ATTIKON”, Medical School, National and Kapodistrian University of Athens, 124 62 Athens, GreeceFirst Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 115 28 Athens, GreeceThird Department of Obstetrics and Gynecology, University General Hospital “ATTIKON”, Medical School, National and Kapodistrian University of Athens, 124 62 Athens, GreeceBackground: Glutathione S-transferase (GST) M1 belongs to a family of detoxification enzymes and deficiency in enzyme activity is due to a homozygous deletion of the <i>GSTM1</i> gene. Several studies reveal a possible correlation between female infertility and <i>GSTM1</i> polymorphisms. The aim of this study is to investigate the effect of the <i>GSTM1</i>-null polymorphism in female infertility as well as in IVF parameters. Methods: In the study group 125 women were classified as infertile according to WHO and 49 women with at least one successful pregnancy and no miscarriages, as control group. Genomic DNA from blood samples was isolated and PCR amplification was applied to determine the presence of <i>GSTM1</i>-null genotype. Results: Data analysis demonstrated a statistically significant higher presence of <i>GSTM1</i>-null variant in the infertile group compared to the control group. In a subgroup analysis of the infertile group, the estradiol levels, the number of fertilized oocytes as well as the number and the quality of the cumulus-oocyte complex, were statistically significant higher in women detected with the wildtype of <i>GSTM1</i> gene compared to those who had the <i>GSTM1</i> null genotype (deletion). Conclusions: Our study results propose a possible involvement of GMST1 in female infertility and may help elucidate possible interactions between the microenvironment of oocytes and the oxidative stress.https://www.mdpi.com/2077-0383/12/23/7269female infertility<i>GSTM1</i> geneARTin vitro fertilization (IVF)oxidative stress |
| spellingShingle | Ioannis Chrysanthopoulos Angelos Petsavas Despoina Mavrogianni Anastasios Potiris Nikolaos Machairiotis Eirini Drakaki Dionysios Vrachnis Pavlos Machairoudias Theodoros Karampitsakos Paraskevas Perros Christos Koratzanis Peter Drakakis Sofoklis Stavros Detection of <i>GSTM1</i>-null Genotype in Women Undergoing IVF Treatment Journal of Clinical Medicine female infertility <i>GSTM1</i> gene ART in vitro fertilization (IVF) oxidative stress |
| title | Detection of <i>GSTM1</i>-null Genotype in Women Undergoing IVF Treatment |
| title_full | Detection of <i>GSTM1</i>-null Genotype in Women Undergoing IVF Treatment |
| title_fullStr | Detection of <i>GSTM1</i>-null Genotype in Women Undergoing IVF Treatment |
| title_full_unstemmed | Detection of <i>GSTM1</i>-null Genotype in Women Undergoing IVF Treatment |
| title_short | Detection of <i>GSTM1</i>-null Genotype in Women Undergoing IVF Treatment |
| title_sort | detection of i gstm1 i null genotype in women undergoing ivf treatment |
| topic | female infertility <i>GSTM1</i> gene ART in vitro fertilization (IVF) oxidative stress |
| url | https://www.mdpi.com/2077-0383/12/23/7269 |
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