Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors.
The mammalian JAM family is composed of three cell surface receptors. Interactions between the proteins have well-characterised roles in inflammation and tight junction formation, but little is known about their function in early development. Recently, we identified a role for jamb and jamc in zebra...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040810&type=printable |
| _version_ | 1826801313795538944 |
|---|---|
| author | Gareth T Powell Gavin J Wright |
| author_facet | Gareth T Powell Gavin J Wright |
| author_sort | Gareth T Powell |
| collection | DOAJ |
| description | The mammalian JAM family is composed of three cell surface receptors. Interactions between the proteins have well-characterised roles in inflammation and tight junction formation, but little is known about their function in early development. Recently, we identified a role for jamb and jamc in zebrafish myocyte fusion. Genome duplication in the teleost lineage raised the possibility that additional JAM family paralogues may also function in muscle development. To address this, we searched the zebrafish genome to identify potential paralogues and confirmed their homology, bringing the total number of zebrafish jam family members to six. We then compared the physical binding properties of each paralogue by surface plasmon resonance and determined the gene expression patterns of all zebrafish jam genes at different stages of development. Our results suggest a significant sub-functionalisation of JAM-B and JAM-C orthologues with respect to binding strength (but not specificity) and gene expression. The paralogous genes, jamb2 and jamc2, were not detected in the somites or myotome of wild-type embryos. We conclude that it is unlikely that the paralogues have a function in primary myogenesis. |
| first_indexed | 2024-12-11T22:54:40Z |
| format | Article |
| id | doaj.art-b1eccc9d15294721b9492abdefba4157 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-17T01:04:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b1eccc9d15294721b9492abdefba41572025-02-19T05:31:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e4081010.1371/journal.pone.0040810Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors.Gareth T PowellGavin J WrightThe mammalian JAM family is composed of three cell surface receptors. Interactions between the proteins have well-characterised roles in inflammation and tight junction formation, but little is known about their function in early development. Recently, we identified a role for jamb and jamc in zebrafish myocyte fusion. Genome duplication in the teleost lineage raised the possibility that additional JAM family paralogues may also function in muscle development. To address this, we searched the zebrafish genome to identify potential paralogues and confirmed their homology, bringing the total number of zebrafish jam family members to six. We then compared the physical binding properties of each paralogue by surface plasmon resonance and determined the gene expression patterns of all zebrafish jam genes at different stages of development. Our results suggest a significant sub-functionalisation of JAM-B and JAM-C orthologues with respect to binding strength (but not specificity) and gene expression. The paralogous genes, jamb2 and jamc2, were not detected in the somites or myotome of wild-type embryos. We conclude that it is unlikely that the paralogues have a function in primary myogenesis.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040810&type=printable |
| spellingShingle | Gareth T Powell Gavin J Wright Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. PLoS ONE |
| title | Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. |
| title_full | Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. |
| title_fullStr | Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. |
| title_full_unstemmed | Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. |
| title_short | Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. |
| title_sort | genomic organisation embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040810&type=printable |
| work_keys_str_mv | AT garethtpowell genomicorganisationembryonicexpressionandbiochemicalinteractionsofthezebrafishjunctionaladhesionmoleculefamilyofreceptors AT gavinjwright genomicorganisationembryonicexpressionandbiochemicalinteractionsofthezebrafishjunctionaladhesionmoleculefamilyofreceptors |