Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering
Abstract Background To mimic the muscle inspired cells adhesion through proteins secretion, the lamination of collagen–hydroxyapatite nanorod (nHA) composite nanofibers has been carried out successfully on polydopamine (PDA)-coated microfibrous polylactide-co-glycolide (PLGA) fabrics. The lamination...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2017-06-01
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| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40824-017-0097-3 |
| _version_ | 1797281486459633664 |
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| author | Gi-Wan Kwon Kailash Chandra Gupta Kyung-Hye Jung Inn-Kyu Kang |
| author_facet | Gi-Wan Kwon Kailash Chandra Gupta Kyung-Hye Jung Inn-Kyu Kang |
| author_sort | Gi-Wan Kwon |
| collection | DOAJ |
| description | Abstract Background To mimic the muscle inspired cells adhesion through proteins secretion, the lamination of collagen–hydroxyapatite nanorod (nHA) composite nanofibers has been carried out successfully on polydopamine (PDA)-coated microfibrous polylactide-co-glycolide (PLGA) fabrics. The lamination of collagen-hydroxyapatite composite nanofibers on polydopamine-coated microfibrous PLGA fabrics was carried through electrospinning the solution of collagen containing L-glutamic acid-grafted hydroxyapatite nanorods (nHA-GA) at a flow rate of 1.5 mL/h and an applied voltage of 15 kV. Results In comparison to pristine PLGA, dopamine-coated PLGA and collagen-hydroxyapatite composite nanofiber lamination has produced more wettable surfaces and surface wettability is found to higher with dopamine-coated PLGA fabrics then pristine PLGA. The SEM micrographs have clearly indicated that the lamination of polydopamine-coated PLGA fabric with collagen-hydroxyapatite composite nanofibers has shown increased adhesion of MC3T3E1 cells in comparison to pristine PLGA fabrics. Conclusion The results of these studies have clearly demonstrated that collagen-nHA composites fibers may be used to create bioactive 3D scaffolds using PLGA as an architectural support agent. |
| first_indexed | 2024-03-07T16:57:17Z |
| format | Article |
| id | doaj.art-b1ed8e87a6a34225b93340142baa7232 |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T16:57:17Z |
| publishDate | 2017-06-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-b1ed8e87a6a34225b93340142baa72322024-03-03T03:40:50ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242017-06-0121111210.1186/s40824-017-0097-3Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineeringGi-Wan Kwon0Kailash Chandra Gupta1Kyung-Hye Jung2Inn-Kyu Kang3Department of Polymer Science and Engineering, Kyungpook National UniversityDepartment of Polymer Science and Engineering, Kyungpook National UniversityDepartment of Advanced Materials and Chemical Engineering,Catholic University of DaeguDepartment of Polymer Science and Engineering, Kyungpook National UniversityAbstract Background To mimic the muscle inspired cells adhesion through proteins secretion, the lamination of collagen–hydroxyapatite nanorod (nHA) composite nanofibers has been carried out successfully on polydopamine (PDA)-coated microfibrous polylactide-co-glycolide (PLGA) fabrics. The lamination of collagen-hydroxyapatite composite nanofibers on polydopamine-coated microfibrous PLGA fabrics was carried through electrospinning the solution of collagen containing L-glutamic acid-grafted hydroxyapatite nanorods (nHA-GA) at a flow rate of 1.5 mL/h and an applied voltage of 15 kV. Results In comparison to pristine PLGA, dopamine-coated PLGA and collagen-hydroxyapatite composite nanofiber lamination has produced more wettable surfaces and surface wettability is found to higher with dopamine-coated PLGA fabrics then pristine PLGA. The SEM micrographs have clearly indicated that the lamination of polydopamine-coated PLGA fabric with collagen-hydroxyapatite composite nanofibers has shown increased adhesion of MC3T3E1 cells in comparison to pristine PLGA fabrics. Conclusion The results of these studies have clearly demonstrated that collagen-nHA composites fibers may be used to create bioactive 3D scaffolds using PLGA as an architectural support agent.http://link.springer.com/article/10.1186/s40824-017-0097-3CollagenPLGAPolydopamineHydroxyapatite nanorods and Scaffolds |
| spellingShingle | Gi-Wan Kwon Kailash Chandra Gupta Kyung-Hye Jung Inn-Kyu Kang Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering Biomaterials Research Collagen PLGA Polydopamine Hydroxyapatite nanorods and Scaffolds |
| title | Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering |
| title_full | Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering |
| title_fullStr | Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering |
| title_full_unstemmed | Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering |
| title_short | Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering |
| title_sort | lamination of microfibrous plga fabric by electrospinning a layer of collagen hydroxyapatite composite nanofibers for bone tissue engineering |
| topic | Collagen PLGA Polydopamine Hydroxyapatite nanorods and Scaffolds |
| url | http://link.springer.com/article/10.1186/s40824-017-0097-3 |
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