Dual antibody strategy for high-resolution imaging of murine Purkinje cells and their dendrites across multiple layers

Dual antibody strategy for high-resolution imaging of murine Purkinje cells and their dendrites across multiple layers

Summary: Despite being among the largest neurons in the mammalian brain, Purkinje cells are difficult to visualize and trace via immunofluorescence because their dendritic arbors extend through several cerebellar layers. This protocol describes a two-antibody strategy we developed to study Purkinje...

Full description

Bibliographic Details
Main Authors: Salvatore Botta, Alexei Chemiakine, Vincenzo A. Gennarino
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Genetics
Microscopy
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003070

Similar Items

Similar Items