New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization
As plants are sessile they need a very efficient system for repairing damage done by external or internal mutagens to their DNA. Mismatch repair (MMR) is one of the systems that maintain genome integrity and prevent homeologous recombination. In all eukaryotes mismatches are recognized by evolutiona...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00003/full |
| _version_ | 1818510749516955648 |
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| author | Elena Rakosy-Tican Enikö Lörincz-Besenyei Enikö Lörincz-Besenyei Imola Molnár Ramona Thieme Frank Hartung Thorben Sprink Olga Antonova Ivan Famelaer Geert Angenon Adriana Aurori |
| author_facet | Elena Rakosy-Tican Enikö Lörincz-Besenyei Enikö Lörincz-Besenyei Imola Molnár Ramona Thieme Frank Hartung Thorben Sprink Olga Antonova Ivan Famelaer Geert Angenon Adriana Aurori |
| author_sort | Elena Rakosy-Tican |
| collection | DOAJ |
| description | As plants are sessile they need a very efficient system for repairing damage done by external or internal mutagens to their DNA. Mismatch repair (MMR) is one of the systems that maintain genome integrity and prevent homeologous recombination. In all eukaryotes mismatches are recognized by evolutionary conserved MSH proteins often acting as heterodimers, the constant component of which is MSH2. Changes affecting the function of MSH2 gene may induce a ‘mutator’ phenotype and microsatellite instability (MSI), as is demonstrated in MSH2 knock-out and silenced lines of Arabidopsis thaliana. The goal of this study was to screen for ‘mutator’ phenotypes in somatic hybrids between potato cvs. ‘Delikat’ and ‘Désirée’ and MMR deficient Solanum chacoense transformed using antisense (AS) or dominant negative mutant (DN) AtMSH2 genes. The results demonstrate that first generation fusion hybrids have a range of morphological abnormalities caused by uniparental MMR deficiency; these mutant phenotypes include: dwarf or gigantic plants; bushiness; curled, small, large or abnormal leaves; a deterioration in chloroplast structure; small deep-purple tubers and early dehiscent flowers. Forty percent of the viable somatic hybrids planted in a greenhouse, (10 out of 25 genotypes) had mutant phenotypes accompanied by MSI. The majority of the hybrids with ‘mutator’ phenotypes cultured on media containing kanamycin developed roots so sustaining the presence of selectable marker gene nptII, from the initial constructs. Here for the first time, MMR deficiency combined with somatic hybridization, are used to induce new phenotypes in plants, which supports the role of MMR deficiency in increasing introgressions between two related species. |
| first_indexed | 2024-12-10T23:24:17Z |
| format | Article |
| id | doaj.art-b1f0de7a996c44419e2cc0e471883859 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T23:24:17Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b1f0de7a996c44419e2cc0e4718838592022-12-22T01:29:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-01-011010.3389/fpls.2019.00003419933New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic HybridizationElena Rakosy-Tican0Enikö Lörincz-Besenyei1Enikö Lörincz-Besenyei2Imola Molnár3Ramona Thieme4Frank Hartung5Thorben Sprink6Olga Antonova7Ivan Famelaer8Geert Angenon9Adriana Aurori10Plant Genetic Engineering Group, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, RomaniaPlant Genetic Engineering Group, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, RomaniaInstitute for Biosafety in Plant Biotechnology, Julius Kühn-Institut, Quedlinburg, GermanyPlant Genetic Engineering Group, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, RomaniaInstitute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, GermanyInstitute for Biosafety in Plant Biotechnology, Julius Kühn-Institut, Quedlinburg, GermanyInstitute for Biosafety in Plant Biotechnology, Julius Kühn-Institut, Quedlinburg, GermanyDepartment of Plant Biotechnology, N.I. Vavilov Institute of Plant Industry, St. Petersburg, RussiaLaboratory of Plant Genetics, Vrije Universiteit Brussel, Brussels, BelgiumLaboratory of Plant Genetics, Vrije Universiteit Brussel, Brussels, BelgiumPlant Genetic Engineering Group, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, RomaniaAs plants are sessile they need a very efficient system for repairing damage done by external or internal mutagens to their DNA. Mismatch repair (MMR) is one of the systems that maintain genome integrity and prevent homeologous recombination. In all eukaryotes mismatches are recognized by evolutionary conserved MSH proteins often acting as heterodimers, the constant component of which is MSH2. Changes affecting the function of MSH2 gene may induce a ‘mutator’ phenotype and microsatellite instability (MSI), as is demonstrated in MSH2 knock-out and silenced lines of Arabidopsis thaliana. The goal of this study was to screen for ‘mutator’ phenotypes in somatic hybrids between potato cvs. ‘Delikat’ and ‘Désirée’ and MMR deficient Solanum chacoense transformed using antisense (AS) or dominant negative mutant (DN) AtMSH2 genes. The results demonstrate that first generation fusion hybrids have a range of morphological abnormalities caused by uniparental MMR deficiency; these mutant phenotypes include: dwarf or gigantic plants; bushiness; curled, small, large or abnormal leaves; a deterioration in chloroplast structure; small deep-purple tubers and early dehiscent flowers. Forty percent of the viable somatic hybrids planted in a greenhouse, (10 out of 25 genotypes) had mutant phenotypes accompanied by MSI. The majority of the hybrids with ‘mutator’ phenotypes cultured on media containing kanamycin developed roots so sustaining the presence of selectable marker gene nptII, from the initial constructs. Here for the first time, MMR deficiency combined with somatic hybridization, are used to induce new phenotypes in plants, which supports the role of MMR deficiency in increasing introgressions between two related species.https://www.frontiersin.org/article/10.3389/fpls.2019.00003/fullantisense strategyAtMSH2 genedominant negative mutantmicrosatellite instabilitymismatch repair deficiency‘mutator’ phenotype |
| spellingShingle | Elena Rakosy-Tican Enikö Lörincz-Besenyei Enikö Lörincz-Besenyei Imola Molnár Ramona Thieme Frank Hartung Thorben Sprink Olga Antonova Ivan Famelaer Geert Angenon Adriana Aurori New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization Frontiers in Plant Science antisense strategy AtMSH2 gene dominant negative mutant microsatellite instability mismatch repair deficiency ‘mutator’ phenotype |
| title | New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization |
| title_full | New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization |
| title_fullStr | New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization |
| title_full_unstemmed | New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization |
| title_short | New Phenotypes of Potato Co-induced by Mismatch Repair Deficiency and Somatic Hybridization |
| title_sort | new phenotypes of potato co induced by mismatch repair deficiency and somatic hybridization |
| topic | antisense strategy AtMSH2 gene dominant negative mutant microsatellite instability mismatch repair deficiency ‘mutator’ phenotype |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00003/full |
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