Genotyping Canadian <i>Cyclospora cayetanensis</i> Isolates to Supplement Cyclosporiasis Outbreak Investigations
<i>Cyclospora cayetanensis</i> is an emerging foodborne parasite that causes cyclosporiasis, an enteric disease of humans. Domestically acquired outbreaks have been reported in Canada every spring or summer since 2013. To date, investigations into the potential sources of infection have...
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/447 |
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| author | Christine A. Yanta John R. Barta Antoine Corbeil Hervé Menan Karine Thivierge Robert Needle Muhammad Morshed Brent R. Dixon James D. Wasmuth Rebecca A. Guy |
| author_facet | Christine A. Yanta John R. Barta Antoine Corbeil Hervé Menan Karine Thivierge Robert Needle Muhammad Morshed Brent R. Dixon James D. Wasmuth Rebecca A. Guy |
| author_sort | Christine A. Yanta |
| collection | DOAJ |
| description | <i>Cyclospora cayetanensis</i> is an emerging foodborne parasite that causes cyclosporiasis, an enteric disease of humans. Domestically acquired outbreaks have been reported in Canada every spring or summer since 2013. To date, investigations into the potential sources of infection have relied solely on epidemiological data. To supplement the epidemiological data with genetic information, we genotyped 169 Canadian cyclosporiasis cases from stool specimens collected from 2010 to 2021 using an existing eight-marker targeted amplicon deep (TADS) scheme specific to <i>C. cayetanensis</i> as previously described by the US Centers for Disease Control and Prevention (CDC). This is the first study to genotype Canadian <i>Cyclospora cayetanensis</i> isolates, and it focuses on evaluating the genotyping performance and genetic clustering. Genotyping information was successfully collected with at least part of one of the markers in the TADS assay for 97.9% of specimens, and 81.1% of cyclosporiasis cases met the minimum requirements to genetically cluster into 20 groups. The performance of the scheme suggests that examining cyclosporiasis cases genetically will be a valuable tool for supplementing epidemiological outbreak investigations and to minimize further infections. Further research is required to expand the number of discriminatory markers to improve genetic clustering. |
| first_indexed | 2024-03-09T21:23:01Z |
| format | Article |
| id | doaj.art-b1f3312f77a047729e688074c7793fd1 |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:01Z |
| publishDate | 2022-02-01 |
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| series | Microorganisms |
| spelling | doaj.art-b1f3312f77a047729e688074c7793fd12023-11-23T21:16:21ZengMDPI AGMicroorganisms2076-26072022-02-0110244710.3390/microorganisms10020447Genotyping Canadian <i>Cyclospora cayetanensis</i> Isolates to Supplement Cyclosporiasis Outbreak InvestigationsChristine A. Yanta0John R. Barta1Antoine Corbeil2Hervé Menan3Karine Thivierge4Robert Needle5Muhammad Morshed6Brent R. Dixon7James D. Wasmuth8Rebecca A. Guy9National Microbiology Laboratory at Guelph, Division of Enteric Diseases, Public Health Agency of Canada, Guelph, ON N1G 3W4, CanadaDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, CanadaPublic Health Ontario, Toronto, ON M5G 1M1, CanadaQuébec Public Health Laboratory, Ste-Anne-de-Bellevue, QC H9X 3R5, CanadaQuébec Public Health Laboratory, Ste-Anne-de-Bellevue, QC H9X 3R5, CanadaPublic Health and Microbiology Laboratory, Eastern Health, St John’s, NL A1A 3Z9, CanadaBC Centre for Disease Control Public Health Laboratory, Vancouver, BC V5Z 4R4, CanadaBureau of Microbial Hazards, Food Directorate, Health Canada, Ottawa, ON K1A 0K9, CanadaDepartment of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4Z6, CanadaNational Microbiology Laboratory at Guelph, Division of Enteric Diseases, Public Health Agency of Canada, Guelph, ON N1G 3W4, Canada<i>Cyclospora cayetanensis</i> is an emerging foodborne parasite that causes cyclosporiasis, an enteric disease of humans. Domestically acquired outbreaks have been reported in Canada every spring or summer since 2013. To date, investigations into the potential sources of infection have relied solely on epidemiological data. To supplement the epidemiological data with genetic information, we genotyped 169 Canadian cyclosporiasis cases from stool specimens collected from 2010 to 2021 using an existing eight-marker targeted amplicon deep (TADS) scheme specific to <i>C. cayetanensis</i> as previously described by the US Centers for Disease Control and Prevention (CDC). This is the first study to genotype Canadian <i>Cyclospora cayetanensis</i> isolates, and it focuses on evaluating the genotyping performance and genetic clustering. Genotyping information was successfully collected with at least part of one of the markers in the TADS assay for 97.9% of specimens, and 81.1% of cyclosporiasis cases met the minimum requirements to genetically cluster into 20 groups. The performance of the scheme suggests that examining cyclosporiasis cases genetically will be a valuable tool for supplementing epidemiological outbreak investigations and to minimize further infections. Further research is required to expand the number of discriminatory markers to improve genetic clustering.https://www.mdpi.com/2076-2607/10/2/447<i>Cyclospora cayetanensis</i>cyclosporiasisgenotypingtargeted amplicon deep sequencing (TADS)Canadian outbreaksfoodborne pathogen |
| spellingShingle | Christine A. Yanta John R. Barta Antoine Corbeil Hervé Menan Karine Thivierge Robert Needle Muhammad Morshed Brent R. Dixon James D. Wasmuth Rebecca A. Guy Genotyping Canadian <i>Cyclospora cayetanensis</i> Isolates to Supplement Cyclosporiasis Outbreak Investigations Microorganisms <i>Cyclospora cayetanensis</i> cyclosporiasis genotyping targeted amplicon deep sequencing (TADS) Canadian outbreaks foodborne pathogen |
| title | Genotyping Canadian <i>Cyclospora cayetanensis</i> Isolates to Supplement Cyclosporiasis Outbreak Investigations |
| title_full | Genotyping Canadian <i>Cyclospora cayetanensis</i> Isolates to Supplement Cyclosporiasis Outbreak Investigations |
| title_fullStr | Genotyping Canadian <i>Cyclospora cayetanensis</i> Isolates to Supplement Cyclosporiasis Outbreak Investigations |
| title_full_unstemmed | Genotyping Canadian <i>Cyclospora cayetanensis</i> Isolates to Supplement Cyclosporiasis Outbreak Investigations |
| title_short | Genotyping Canadian <i>Cyclospora cayetanensis</i> Isolates to Supplement Cyclosporiasis Outbreak Investigations |
| title_sort | genotyping canadian i cyclospora cayetanensis i isolates to supplement cyclosporiasis outbreak investigations |
| topic | <i>Cyclospora cayetanensis</i> cyclosporiasis genotyping targeted amplicon deep sequencing (TADS) Canadian outbreaks foodborne pathogen |
| url | https://www.mdpi.com/2076-2607/10/2/447 |
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