Transmitted light pH optode for small sample volumes
An innovative concept of a low-cost pH optode with working volumes of less than 150 µL is presented. The pH monitoring is based on the color changing effect of pH indicators. The optode includes an RGB color sensor patch TCS34725 from Adafruit, a controllable LED and reactor slides and is addres...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2017-10-01
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| Series: | Journal of Sensors and Sensor Systems |
| Online Access: | https://www.j-sens-sens-syst.net/6/351/2017/jsss-6-351-2017.pdf |
| Summary: | An innovative concept of a low-cost pH optode with working volumes of less
than 150 µL is presented. The pH monitoring is based on the color
changing effect of pH indicators. The optode includes an RGB color sensor
patch TCS34725 from Adafruit, a controllable LED and reactor slides and is
addressed by a self-written LabVIEW<span style="position:relative; bottom:0.5em; " class="text">©</span> software.
Utilizing the hue value of the HSV color model, it is possible to analyze the
color change of the indicator and estimate the pH value of the analyzed
samples by exploiting sigmoidal fit models. Measurements carried out with
phenol red and DMEM (Dulbecco's Modified Eagle's Medium) reported a standard
error of calibration in the physiologic pH range (6.5–7.5) of ±0.04 pH
units. |
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| ISSN: | 2194-8771 2194-878X |