Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells
Both pathogenic and non-pathogenic Mycobacteria can induce the differentiation of immune cells into dendritic cells (DC) or DC-like cells. In addition, pathogenic Mycobacteria is found to stimulate cell differentiation in the nerves system. Whether non-pathogenic Mycobacteria interacts with nerve ce...
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2018.00201/full |
| _version_ | 1819060032963084288 |
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| author | Xinwei Feng Junfeng Lu Zitian He Yidan Wang Fangfang Qi Rongbiao Pi Ge Zhang |
| author_facet | Xinwei Feng Junfeng Lu Zitian He Yidan Wang Fangfang Qi Rongbiao Pi Ge Zhang |
| author_sort | Xinwei Feng |
| collection | DOAJ |
| description | Both pathogenic and non-pathogenic Mycobacteria can induce the differentiation of immune cells into dendritic cells (DC) or DC-like cells. In addition, pathogenic Mycobacteria is found to stimulate cell differentiation in the nerves system. Whether non-pathogenic Mycobacteria interacts with nerve cells remains unknown. In this study, we found that co-incubation with fast-growing Mycobacteria smegmatis induced neuron-like morphological changes of PC12 and C17.2 cells. Moreover, the M. smegmatis culture supernatant which was ultrafiltrated through a membrane with a 10 kDa cut-off, induced neurite outgrowth and differentiation in an autophagy-independent pathway in PC12 and C17.2 cells. Further analysis showed that IFN-γ production and activation of the PI3K-Akt signaling pathway were involved in the neural differentiation. In conclusion, our finding demonstrated that non-pathogenic M. smegmatis was able to promote neuronal differentiation by its extracellular proteins, which might provide a novel therapeutic strategy for the treatment of neurodegenerative disorders. |
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| language | English |
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| publishDate | 2018-06-01 |
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| spelling | doaj.art-b1f48e39c6df43949b5a483602fd5b572022-12-21T19:00:48ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882018-06-01810.3389/fcimb.2018.00201329481Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 CellsXinwei Feng0Junfeng Lu1Zitian He2Yidan Wang3Fangfang Qi4Rongbiao Pi5Ge Zhang6Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaDepartment of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaDepartment of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaDepartment of Biotechnology, School of McCormick Engineering, Northwestern University, Evanston, IL, United StatesDepartment of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaDepartment of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaDepartment of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, ChinaBoth pathogenic and non-pathogenic Mycobacteria can induce the differentiation of immune cells into dendritic cells (DC) or DC-like cells. In addition, pathogenic Mycobacteria is found to stimulate cell differentiation in the nerves system. Whether non-pathogenic Mycobacteria interacts with nerve cells remains unknown. In this study, we found that co-incubation with fast-growing Mycobacteria smegmatis induced neuron-like morphological changes of PC12 and C17.2 cells. Moreover, the M. smegmatis culture supernatant which was ultrafiltrated through a membrane with a 10 kDa cut-off, induced neurite outgrowth and differentiation in an autophagy-independent pathway in PC12 and C17.2 cells. Further analysis showed that IFN-γ production and activation of the PI3K-Akt signaling pathway were involved in the neural differentiation. In conclusion, our finding demonstrated that non-pathogenic M. smegmatis was able to promote neuronal differentiation by its extracellular proteins, which might provide a novel therapeutic strategy for the treatment of neurodegenerative disorders.https://www.frontiersin.org/article/10.3389/fcimb.2018.00201/fullMycobacterium smegmatisneurite outgrowthdifferentiationautophagyPI3K-Akt pathway |
| spellingShingle | Xinwei Feng Junfeng Lu Zitian He Yidan Wang Fangfang Qi Rongbiao Pi Ge Zhang Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells Frontiers in Cellular and Infection Microbiology Mycobacterium smegmatis neurite outgrowth differentiation autophagy PI3K-Akt pathway |
| title | Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells |
| title_full | Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells |
| title_fullStr | Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells |
| title_full_unstemmed | Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells |
| title_short | Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells |
| title_sort | mycobacterium smegmatis induces neurite outgrowth and differentiation in an autophagy independent manner in pc12 and c17 2 cells |
| topic | Mycobacterium smegmatis neurite outgrowth differentiation autophagy PI3K-Akt pathway |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2018.00201/full |
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