Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain
Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneum...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.739461/full |
| _version_ | 1818934561510260736 |
|---|---|
| author | Xuemei Yang Ning Dong Xiaoxuan Liu Chen Yang Lianwei Ye Edward Wai-Chi Chan Edward Wai-Chi Chan Rong Zhang Sheng Chen |
| author_facet | Xuemei Yang Ning Dong Xiaoxuan Liu Chen Yang Lianwei Ye Edward Wai-Chi Chan Edward Wai-Chi Chan Rong Zhang Sheng Chen |
| author_sort | Xuemei Yang |
| collection | DOAJ |
| description | Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneumoniae strain which harbored an IncFIB/IncHI1B type virulence plasmid and an IncFII/IncR type blaKPC–2-bearing plasmid. The virulence plasmid was found to be conjugative and harbored a 35-kbp fragment including aerobactin encoding cluster from virulence plasmid pLVPK and multiple resistance genes, resulting in a mosaic multi-drug resistance and virulence plasmid. This virulence plasmid could be transferred via conjugation to Escherichia coli and K. pneumoniae strains alone as well as together with the blaKPC–2-bearing plasmid. Co-transmission of virulence and blaKPC–2-bearing plasmids would directly convert a classic K. pneumoniae strain into CR-HvKP strain, leading to a sharp increase in the prevalence of CR-HvKP in clinical settings, which poses a great threat to human health. |
| first_indexed | 2024-12-20T05:06:14Z |
| format | Article |
| id | doaj.art-b1f4cce6b7a74d5e9e89d27da48dfb5e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T05:06:14Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-b1f4cce6b7a74d5e9e89d27da48dfb5e2022-12-21T19:52:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.739461739461Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae StrainXuemei Yang0Ning Dong1Xiaoxuan Liu2Chen Yang3Lianwei Ye4Edward Wai-Chi Chan5Edward Wai-Chi Chan6Rong Zhang7Sheng Chen8Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaState Key Lab of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, ChinaDepartment of Clinical Laboratory, School of Medicine, Second Affiliated Hospital of Zhejiang University, Hangzhou, ChinaDepartment of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaCarbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains have been increasingly reported, and it is important to understand the evolutionary mechanisms of these highly pathogenic and resistant bacterial pathogens. In this study, we characterized a ST11 carbapenem-resistant K. pneumoniae strain which harbored an IncFIB/IncHI1B type virulence plasmid and an IncFII/IncR type blaKPC–2-bearing plasmid. The virulence plasmid was found to be conjugative and harbored a 35-kbp fragment including aerobactin encoding cluster from virulence plasmid pLVPK and multiple resistance genes, resulting in a mosaic multi-drug resistance and virulence plasmid. This virulence plasmid could be transferred via conjugation to Escherichia coli and K. pneumoniae strains alone as well as together with the blaKPC–2-bearing plasmid. Co-transmission of virulence and blaKPC–2-bearing plasmids would directly convert a classic K. pneumoniae strain into CR-HvKP strain, leading to a sharp increase in the prevalence of CR-HvKP in clinical settings, which poses a great threat to human health.https://www.frontiersin.org/articles/10.3389/fmicb.2021.739461/fullKlebsiella pneumoniaconjugative virulence plasmidblaKPC-bearing plasmidtransmissionCR-HvKPIncFIB/IncHI1B |
| spellingShingle | Xuemei Yang Ning Dong Xiaoxuan Liu Chen Yang Lianwei Ye Edward Wai-Chi Chan Edward Wai-Chi Chan Rong Zhang Sheng Chen Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain Frontiers in Microbiology Klebsiella pneumonia conjugative virulence plasmid blaKPC-bearing plasmid transmission CR-HvKP IncFIB/IncHI1B |
| title | Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain |
| title_full | Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain |
| title_fullStr | Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain |
| title_full_unstemmed | Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain |
| title_short | Co-conjugation of Virulence Plasmid and KPC Plasmid in a Clinical Klebsiella pneumoniae Strain |
| title_sort | co conjugation of virulence plasmid and kpc plasmid in a clinical klebsiella pneumoniae strain |
| topic | Klebsiella pneumonia conjugative virulence plasmid blaKPC-bearing plasmid transmission CR-HvKP IncFIB/IncHI1B |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.739461/full |
| work_keys_str_mv | AT xuemeiyang coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain AT ningdong coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain AT xiaoxuanliu coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain AT chenyang coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain AT lianweiye coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain AT edwardwaichichan coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain AT edwardwaichichan coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain AT rongzhang coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain AT shengchen coconjugationofvirulenceplasmidandkpcplasmidinaclinicalklebsiellapneumoniaestrain |