Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus
Although pathogens must infect differentiated host cells that exhibit substantial diversity, documenting the consequences of infection against this heterogeneity is challenging. Single-cell mass cytometry permits deep profiling based on combinatorial expression of surface and intracellular proteins....
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-07-01
|
| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714004938 |
| _version_ | 1819069623920754688 |
|---|---|
| author | Nandini Sen Gourab Mukherjee Adrish Sen Sean C. Bendall Phillip Sung Garry P. Nolan Ann M. Arvin |
| author_facet | Nandini Sen Gourab Mukherjee Adrish Sen Sean C. Bendall Phillip Sung Garry P. Nolan Ann M. Arvin |
| author_sort | Nandini Sen |
| collection | DOAJ |
| description | Although pathogens must infect differentiated host cells that exhibit substantial diversity, documenting the consequences of infection against this heterogeneity is challenging. Single-cell mass cytometry permits deep profiling based on combinatorial expression of surface and intracellular proteins. We used this method to investigate varicella-zoster virus (VZV) infection of tonsil T cells, which mediate viral transport to skin. Our results indicate that VZV induces a continuum of changes regardless of basal phenotypic and functional T cell characteristics. Contrary to the premise that VZV selectively infects T cells with skin trafficking profiles, VZV infection altered T cell surface proteins to enhance or induce these properties. Zap70 and Akt signaling pathways that trigger such surface changes were activated in VZV-infected naive and memory cells by a T cell receptor (TCR)-independent process. Single-cell mass cytometry is likely to be broadly relevant for demonstrating how intracellular pathogens modulate differentiated cells to support pathogenesis in the natural host. |
| first_indexed | 2024-12-21T16:53:00Z |
| format | Article |
| id | doaj.art-b1f5f45ef0eb4859adbccdc38da69a07 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-21T16:53:00Z |
| publishDate | 2014-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-b1f5f45ef0eb4859adbccdc38da69a072022-12-21T18:56:49ZengElsevierCell Reports2211-12472014-07-018263364510.1016/j.celrep.2014.06.024Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster VirusNandini Sen0Gourab Mukherjee1Adrish Sen2Sean C. Bendall3Phillip Sung4Garry P. Nolan5Ann M. Arvin6Department of Pediatrics, Stanford University, Stanford, CA 94025, USADepartment of Statistics, Stanford University, Stanford, CA 94025, USADepartment of Medicine, Stanford University, Stanford, CA 94025, USADepartment of Microbiology and Immunology, Stanford University, Stanford, CA 94025, USADepartment of Pediatrics, Stanford University, Stanford, CA 94025, USADepartment of Microbiology and Immunology, Stanford University, Stanford, CA 94025, USADepartment of Pediatrics, Stanford University, Stanford, CA 94025, USAAlthough pathogens must infect differentiated host cells that exhibit substantial diversity, documenting the consequences of infection against this heterogeneity is challenging. Single-cell mass cytometry permits deep profiling based on combinatorial expression of surface and intracellular proteins. We used this method to investigate varicella-zoster virus (VZV) infection of tonsil T cells, which mediate viral transport to skin. Our results indicate that VZV induces a continuum of changes regardless of basal phenotypic and functional T cell characteristics. Contrary to the premise that VZV selectively infects T cells with skin trafficking profiles, VZV infection altered T cell surface proteins to enhance or induce these properties. Zap70 and Akt signaling pathways that trigger such surface changes were activated in VZV-infected naive and memory cells by a T cell receptor (TCR)-independent process. Single-cell mass cytometry is likely to be broadly relevant for demonstrating how intracellular pathogens modulate differentiated cells to support pathogenesis in the natural host.http://www.sciencedirect.com/science/article/pii/S2211124714004938 |
| spellingShingle | Nandini Sen Gourab Mukherjee Adrish Sen Sean C. Bendall Phillip Sung Garry P. Nolan Ann M. Arvin Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus Cell Reports |
| title | Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus |
| title_full | Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus |
| title_fullStr | Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus |
| title_full_unstemmed | Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus |
| title_short | Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus |
| title_sort | single cell mass cytometry analysis of human tonsil t cell remodeling by varicella zoster virus |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714004938 |
| work_keys_str_mv | AT nandinisen singlecellmasscytometryanalysisofhumantonsiltcellremodelingbyvaricellazostervirus AT gourabmukherjee singlecellmasscytometryanalysisofhumantonsiltcellremodelingbyvaricellazostervirus AT adrishsen singlecellmasscytometryanalysisofhumantonsiltcellremodelingbyvaricellazostervirus AT seancbendall singlecellmasscytometryanalysisofhumantonsiltcellremodelingbyvaricellazostervirus AT phillipsung singlecellmasscytometryanalysisofhumantonsiltcellremodelingbyvaricellazostervirus AT garrypnolan singlecellmasscytometryanalysisofhumantonsiltcellremodelingbyvaricellazostervirus AT annmarvin singlecellmasscytometryanalysisofhumantonsiltcellremodelingbyvaricellazostervirus |