The Treatment of Keloid Scars via Modulating Heterogeneous Gelatin-Structured Composite Microneedles to Control Transdermal Dual-Drug Release
Keloid scarring is an abnormal scar disease characterised by excessive proliferation of fibroblasts and over-deposition of collagen during wound healing. Although various treatments for keloid scars have been developed, preventive medicine is believed to be a promising strategy. The skin barrier lim...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/20/4436 |
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| author | Yong-Ji Chen Hung-Wei Cheng Wan-Yu Yen Jen-Hao Tsai Chin-Yi Yeh Ching-Jung Chen Jen Tsai Liu San-Yuan Chen Shwu-Jen Chang |
| author_facet | Yong-Ji Chen Hung-Wei Cheng Wan-Yu Yen Jen-Hao Tsai Chin-Yi Yeh Ching-Jung Chen Jen Tsai Liu San-Yuan Chen Shwu-Jen Chang |
| author_sort | Yong-Ji Chen |
| collection | DOAJ |
| description | Keloid scarring is an abnormal scar disease characterised by excessive proliferation of fibroblasts and over-deposition of collagen during wound healing. Although various treatments for keloid scars have been developed, preventive medicine is believed to be a promising strategy. The skin barrier limits the gentle topical administration of medicaments such as creams and hydrogel dressings, resulting in reduced therapeutic efficacy. In recent years, microneedles (MNs) have been regarded as an appreciable device for topical administration without inducing side effects, and they are painless and do not cause bleeding. In this study, an MN patch with controlled transdermal dual-drug release was developed to achieve combinatory treatment of keloid scars using a heterogeneous gelatin-structured composite MN. Gelatin hydrogel was used as a substrate to load gallic acid (GA) and quercetin-loaded amphiphilic gelatin nanoparticles to fabricate dual-drug heterogeneous composite MNs. The results of the insertion test and mechanical properties of the MNs showed that the heterogeneous composite MN patches could be self-pressed into the stratum corneum and control dual-drug release at different time periods. GA was released at an earlier stage to retard the proliferation of fibroblasts, and quercetin was released at a later stage as a strong antioxidant to erase the generation of reactive oxygen species. Furthermore, real-time quantitative polymerase chain reaction data indicated that the gene expression of fibroblasts (such as Col I and III) was downregulated in the dual-drug system. The above results demonstrate that using heterogeneous composite MNs with the combination of dual-drug pharmacology is beneficial for preventing keloid scar formation. |
| first_indexed | 2024-03-09T19:32:55Z |
| format | Article |
| id | doaj.art-b1f633daf31f414ebf8a82d393b0bbbc |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T19:32:55Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b1f633daf31f414ebf8a82d393b0bbbc2023-11-24T02:09:25ZengMDPI AGPolymers2073-43602022-10-011420443610.3390/polym14204436The Treatment of Keloid Scars via Modulating Heterogeneous Gelatin-Structured Composite Microneedles to Control Transdermal Dual-Drug ReleaseYong-Ji Chen0Hung-Wei Cheng1Wan-Yu Yen2Jen-Hao Tsai3Chin-Yi Yeh4Ching-Jung Chen5Jen Tsai Liu6San-Yuan Chen7Shwu-Jen Chang8Department of Biomedical Engineering, I-Shou University, Kaohsiung 82445, TaiwanDepartment of Materials Science and Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, TaiwanDepartment of Biomedical Engineering, I-Shou University, Kaohsiung 82445, TaiwanDepartment of Biomedical Engineering, I-Shou University, Kaohsiung 82445, TaiwanDepartment of Biomedical Engineering, I-Shou University, Kaohsiung 82445, TaiwanResearch Center for Materials Science and Opti-Electronic Technology, School of Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, ChinaResearch Center for Materials Science and Opti-Electronic Technology, College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing 100049, ChinaDepartment of Materials Science and Engineering, National Yang Ming Chiao Tung University, Hsinchu 30010, TaiwanDepartment of Biomedical Engineering, I-Shou University, Kaohsiung 82445, TaiwanKeloid scarring is an abnormal scar disease characterised by excessive proliferation of fibroblasts and over-deposition of collagen during wound healing. Although various treatments for keloid scars have been developed, preventive medicine is believed to be a promising strategy. The skin barrier limits the gentle topical administration of medicaments such as creams and hydrogel dressings, resulting in reduced therapeutic efficacy. In recent years, microneedles (MNs) have been regarded as an appreciable device for topical administration without inducing side effects, and they are painless and do not cause bleeding. In this study, an MN patch with controlled transdermal dual-drug release was developed to achieve combinatory treatment of keloid scars using a heterogeneous gelatin-structured composite MN. Gelatin hydrogel was used as a substrate to load gallic acid (GA) and quercetin-loaded amphiphilic gelatin nanoparticles to fabricate dual-drug heterogeneous composite MNs. The results of the insertion test and mechanical properties of the MNs showed that the heterogeneous composite MN patches could be self-pressed into the stratum corneum and control dual-drug release at different time periods. GA was released at an earlier stage to retard the proliferation of fibroblasts, and quercetin was released at a later stage as a strong antioxidant to erase the generation of reactive oxygen species. Furthermore, real-time quantitative polymerase chain reaction data indicated that the gene expression of fibroblasts (such as Col I and III) was downregulated in the dual-drug system. The above results demonstrate that using heterogeneous composite MNs with the combination of dual-drug pharmacology is beneficial for preventing keloid scar formation.https://www.mdpi.com/2073-4360/14/20/4436keloid scargallic acidquercetinhydrogel microneedlenanocarrier |
| spellingShingle | Yong-Ji Chen Hung-Wei Cheng Wan-Yu Yen Jen-Hao Tsai Chin-Yi Yeh Ching-Jung Chen Jen Tsai Liu San-Yuan Chen Shwu-Jen Chang The Treatment of Keloid Scars via Modulating Heterogeneous Gelatin-Structured Composite Microneedles to Control Transdermal Dual-Drug Release Polymers keloid scar gallic acid quercetin hydrogel microneedle nanocarrier |
| title | The Treatment of Keloid Scars via Modulating Heterogeneous Gelatin-Structured Composite Microneedles to Control Transdermal Dual-Drug Release |
| title_full | The Treatment of Keloid Scars via Modulating Heterogeneous Gelatin-Structured Composite Microneedles to Control Transdermal Dual-Drug Release |
| title_fullStr | The Treatment of Keloid Scars via Modulating Heterogeneous Gelatin-Structured Composite Microneedles to Control Transdermal Dual-Drug Release |
| title_full_unstemmed | The Treatment of Keloid Scars via Modulating Heterogeneous Gelatin-Structured Composite Microneedles to Control Transdermal Dual-Drug Release |
| title_short | The Treatment of Keloid Scars via Modulating Heterogeneous Gelatin-Structured Composite Microneedles to Control Transdermal Dual-Drug Release |
| title_sort | treatment of keloid scars via modulating heterogeneous gelatin structured composite microneedles to control transdermal dual drug release |
| topic | keloid scar gallic acid quercetin hydrogel microneedle nanocarrier |
| url | https://www.mdpi.com/2073-4360/14/20/4436 |
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