Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions
Polycaprolactone (PCL) is a synthetic polymer with good mechanical properties that are useful to produce biomaterials of clinical application. It can be successfully combined with chitosan, which enhances the biomaterial properties through the modulation of molecular and cellular mechanisms. The obj...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/7/1580 |
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| author | Flávia Cristina Zanchetta Rafael Bergamo Trinca Juliany Lino Gomes Silva Jéssica da Silva Cunha Breder Thiago Anselmo Cantarutti Sílvio Roberto Consonni Ângela Maria Moraes Eliana Pereira de Araújo Mario José Abdalla Saad Gary G. Adams Maria Helena Melo Lima |
| author_facet | Flávia Cristina Zanchetta Rafael Bergamo Trinca Juliany Lino Gomes Silva Jéssica da Silva Cunha Breder Thiago Anselmo Cantarutti Sílvio Roberto Consonni Ângela Maria Moraes Eliana Pereira de Araújo Mario José Abdalla Saad Gary G. Adams Maria Helena Melo Lima |
| author_sort | Flávia Cristina Zanchetta |
| collection | DOAJ |
| description | Polycaprolactone (PCL) is a synthetic polymer with good mechanical properties that are useful to produce biomaterials of clinical application. It can be successfully combined with chitosan, which enhances the biomaterial properties through the modulation of molecular and cellular mechanisms. The objective of this study was to evaluate the effects of the use of electrospun fibrous membranes consisting of polycaprolactone (PCL) or polycaprolactone coated with chitosan and poly(ethylene oxide) (PCL+CHI/PEO) on mouse skin lesions. Sixty four Black-57 mice were divided into PCL and PCL+CHI/PEO groups. A 1 cm<sup>2</sup> lesion was made on the animals’ backs, and the membranes were sutured in place. The tissues were extracted on the 3rd, 7th, and 14th days after the lesion. The tissues were analyzed by histology with Hematoxylin and Eosin (H&E) and Sirius Red stains, morphometry, immunohistochemistry, and Western blot. On the 3rd, 6th, and 9th days after the lesion, the PCL+CHI/PEO group showed a higher wound-healing rate (WHR). On the 3 day, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, greater expression of proliferating cell nuclear antigen (PCNA), and smooth muscle actin (α-SMA) (<i>p</i> < 0.05) compared to the PCL group. On the 7th day after the lesion, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, expression of Tumor Necrosis Factor (TNF-α) and PCNA (<i>p</i> < 0.05). In addition, it showed a greater immunolabeling of Monocyte Chemoattractant Protein-1 (MCP-1) and deposition of collagen fibers compared to the PCL group. The PCL+CHI/PEO membrane modulated the increase in the inflammatory infiltrate, the expression of MCP-1, PCNA, and α-SMA in lesions of mice. |
| first_indexed | 2024-03-10T18:26:57Z |
| format | Article |
| id | doaj.art-b1f6b598576c4480a141aef5079bf3ec |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T18:26:57Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b1f6b598576c4480a141aef5079bf3ec2023-11-20T06:57:12ZengMDPI AGPolymers2073-43602020-07-01127158010.3390/polym12071580Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin LesionsFlávia Cristina Zanchetta0Rafael Bergamo Trinca1Juliany Lino Gomes Silva2Jéssica da Silva Cunha Breder3Thiago Anselmo Cantarutti4Sílvio Roberto Consonni5Ângela Maria Moraes6Eliana Pereira de Araújo7Mario José Abdalla Saad8Gary G. Adams9Maria Helena Melo Lima10School of Nursing, University of Campinas, Campinas CEP 13083887, BrazilDepartment of Engineering of Materials and of Bioprocess, School of Chemical Engineering, University of Campinas, Campinas CEP 13083852, BrazilSchool of Nursing, University of Campinas, Campinas CEP 13083887, BrazilSchool of Nursing, University of Campinas, Campinas CEP 13083887, BrazilSchool of Nursing, University of Campinas, Campinas CEP 13083887, BrazilDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas CEP 13083970, BrazilDepartment of Engineering of Materials and of Bioprocess, School of Chemical Engineering, University of Campinas, Campinas CEP 13083852, BrazilSchool of Nursing, University of Campinas, Campinas CEP 13083887, BrazilDepartment of Internal Medicine, University of Campinas, Campinas CEP 13083887, BrazilSchool of Health Sciences, Faculty of Medicine, The University of Nottingham, C Floor, South Block Link, Queen’s Medical Centre, Nottingham NG7 2HA, UKSchool of Nursing, University of Campinas, Campinas CEP 13083887, BrazilPolycaprolactone (PCL) is a synthetic polymer with good mechanical properties that are useful to produce biomaterials of clinical application. It can be successfully combined with chitosan, which enhances the biomaterial properties through the modulation of molecular and cellular mechanisms. The objective of this study was to evaluate the effects of the use of electrospun fibrous membranes consisting of polycaprolactone (PCL) or polycaprolactone coated with chitosan and poly(ethylene oxide) (PCL+CHI/PEO) on mouse skin lesions. Sixty four Black-57 mice were divided into PCL and PCL+CHI/PEO groups. A 1 cm<sup>2</sup> lesion was made on the animals’ backs, and the membranes were sutured in place. The tissues were extracted on the 3rd, 7th, and 14th days after the lesion. The tissues were analyzed by histology with Hematoxylin and Eosin (H&E) and Sirius Red stains, morphometry, immunohistochemistry, and Western blot. On the 3rd, 6th, and 9th days after the lesion, the PCL+CHI/PEO group showed a higher wound-healing rate (WHR). On the 3 day, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, greater expression of proliferating cell nuclear antigen (PCNA), and smooth muscle actin (α-SMA) (<i>p</i> < 0.05) compared to the PCL group. On the 7th day after the lesion, the PCL+CHI/PEO group showed a greater amount of inflammatory infiltrate, expression of Tumor Necrosis Factor (TNF-α) and PCNA (<i>p</i> < 0.05). In addition, it showed a greater immunolabeling of Monocyte Chemoattractant Protein-1 (MCP-1) and deposition of collagen fibers compared to the PCL group. The PCL+CHI/PEO membrane modulated the increase in the inflammatory infiltrate, the expression of MCP-1, PCNA, and α-SMA in lesions of mice.https://www.mdpi.com/2073-4360/12/7/1580C57BL/6J micewound healingwound dressingchitosanpolycaprolactoneelectrospinning |
| spellingShingle | Flávia Cristina Zanchetta Rafael Bergamo Trinca Juliany Lino Gomes Silva Jéssica da Silva Cunha Breder Thiago Anselmo Cantarutti Sílvio Roberto Consonni Ângela Maria Moraes Eliana Pereira de Araújo Mario José Abdalla Saad Gary G. Adams Maria Helena Melo Lima Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions Polymers C57BL/6J mice wound healing wound dressing chitosan polycaprolactone electrospinning |
| title | Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions |
| title_full | Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions |
| title_fullStr | Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions |
| title_full_unstemmed | Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions |
| title_short | Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions |
| title_sort | effects of electrospun fibrous membranes of polycaprolactone and chitosan poly ethylene oxide on mouse acute skin lesions |
| topic | C57BL/6J mice wound healing wound dressing chitosan polycaprolactone electrospinning |
| url | https://www.mdpi.com/2073-4360/12/7/1580 |
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