Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues.
Background: Uncontrolled tumor cell proliferation is a reality in tumor cells, and the progression from a normal cell into a transformed cell probably includes genetic events affecting checkpoints in the cell cycle machinery. Materials and methods: This study investigated the immunoexpression of p16...
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| Format: | Article |
| Language: | English |
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College of Medicine University of Baghdad
2011-01-01
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| Series: | مجلة كلية الطب |
| Subjects: | |
| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/954 |
| _version_ | 1797370170198458368 |
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| author | Abdelameer K. lelo Nidhal abdulmohaimen Faeza aftan |
| author_facet | Abdelameer K. lelo Nidhal abdulmohaimen Faeza aftan |
| author_sort | Abdelameer K. lelo |
| collection | DOAJ |
| description | Background: Uncontrolled tumor cell proliferation is a reality in tumor cells, and the progression from a normal cell into a transformed cell probably includes genetic events affecting checkpoints in the cell cycle machinery.
Materials and methods: This study investigated the immunoexpression of p16INK4a in the paraffin sections from 43-cancers of colorectal tissue (CRC),26-hyperplastic polyps and adenomas(CRHPA) and 35-normal tissues (CRN),using immunohistochemical assay. We correlated the expression patterns with tumor histopathological type, site of the tumor, distance metastasis according to the TNM system.
Results: In colorectal cancer (CRC) patients’ p16INK4a,immunoexpression was detected in 32/43, there was significant association for p16INK4a, which showed high reaction with monoclonal antibodies in the distal colon and rectal area with P value < 0.001. There was no significant correlation between tissue invasion stage and p16INK4a. There was no significant difference in the immunoexpression of p16INK4a in relation to the two groups of patients with the stage of the disease.
Conclusion: p16INK4a, expression have high level in CRHPA than in CRN tissue. Also, their expression in CRC is more than in CRN tissue. In addition, p16INK4a immunoexpression in CRC was significantly more than in CRHPA. |
| first_indexed | 2024-03-08T17:58:33Z |
| format | Article |
| id | doaj.art-b1f7c09fc44e466f96a7d844c63fef3a |
| institution | Directory Open Access Journal |
| issn | 0041-9419 2410-8057 |
| language | English |
| last_indexed | 2024-03-08T17:58:33Z |
| publishDate | 2011-01-01 |
| publisher | College of Medicine University of Baghdad |
| record_format | Article |
| series | مجلة كلية الطب |
| spelling | doaj.art-b1f7c09fc44e466f96a7d844c63fef3a2024-01-02T03:55:11ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572011-01-0152410.32007/jfacmedbagdad.v4445-448%Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues.Abdelameer K. lelo0Nidhal abdulmohaimen1Faeza aftan2Dept. of microbiology, college of medicine, alnahrain University.Dept. of microbiology, college of medicine, alnahrain University.Dept. of pathology, college of medicine, alnahrain University.Background: Uncontrolled tumor cell proliferation is a reality in tumor cells, and the progression from a normal cell into a transformed cell probably includes genetic events affecting checkpoints in the cell cycle machinery. Materials and methods: This study investigated the immunoexpression of p16INK4a in the paraffin sections from 43-cancers of colorectal tissue (CRC),26-hyperplastic polyps and adenomas(CRHPA) and 35-normal tissues (CRN),using immunohistochemical assay. We correlated the expression patterns with tumor histopathological type, site of the tumor, distance metastasis according to the TNM system. Results: In colorectal cancer (CRC) patients’ p16INK4a,immunoexpression was detected in 32/43, there was significant association for p16INK4a, which showed high reaction with monoclonal antibodies in the distal colon and rectal area with P value < 0.001. There was no significant correlation between tissue invasion stage and p16INK4a. There was no significant difference in the immunoexpression of p16INK4a in relation to the two groups of patients with the stage of the disease. Conclusion: p16INK4a, expression have high level in CRHPA than in CRN tissue. Also, their expression in CRC is more than in CRN tissue. In addition, p16INK4a immunoexpression in CRC was significantly more than in CRHPA.http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/954colon cancer, p16INK4a, immunohistochemical assay, cell cycle checkpoints |
| spellingShingle | Abdelameer K. lelo Nidhal abdulmohaimen Faeza aftan Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues. مجلة كلية الطب colon cancer, p16INK4a, immunohistochemical assay, cell cycle checkpoints |
| title | Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues. |
| title_full | Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues. |
| title_fullStr | Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues. |
| title_full_unstemmed | Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues. |
| title_short | Immunohistochemical detection of p16INK4a proteins expression in paraffin embedded sections of colorectal cancer tissues. |
| title_sort | immunohistochemical detection of p16ink4a proteins expression in paraffin embedded sections of colorectal cancer tissues |
| topic | colon cancer, p16INK4a, immunohistochemical assay, cell cycle checkpoints |
| url | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/954 |
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