Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost
Spring radiation frost (SRF) is a severe environmental stress which impairs wheat yield and productivity worldwide. To better understand the mechanism of wheat (Triticum aestivum) responding to SRF, a comparative proteomic analysis was performed to analyze the changes of the key proteins in two whea...
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| Format: | Article |
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Higher Education Press
2014-02-01
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| Series: | Frontiers of Agricultural Science and Engineering |
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| Online Access: | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/2095-7505-2014-1-37.pdf |
| _version_ | 1811233897819668480 |
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| author | Shujuan ZHANG,Guoqi SONG,Yulian LI,Jie GAO,Jiao WANG,Guiju CHEN,Haosheng LI,Genying LI,Zhendong ZHAO |
| author_facet | Shujuan ZHANG,Guoqi SONG,Yulian LI,Jie GAO,Jiao WANG,Guiju CHEN,Haosheng LI,Genying LI,Zhendong ZHAO |
| author_sort | Shujuan ZHANG,Guoqi SONG,Yulian LI,Jie GAO,Jiao WANG,Guiju CHEN,Haosheng LI,Genying LI,Zhendong ZHAO |
| collection | DOAJ |
| description | Spring radiation frost (SRF) is a severe environmental stress which impairs wheat yield and productivity worldwide. To better understand the mechanism of wheat (Triticum aestivum) responding to SRF, a comparative proteomic analysis was performed to analyze the changes of the key proteins in two wheat cultivars Jimai22 and Luyuan301 with high and low tolerance to SRF respectively. A total of 43 differentially expressed proteins (DEPs) which mainly involved in carbohydrate metabolism, amino acid metabolism, resistance proteins and antioxidant enzymes, photosynthesis and cellular respiration proteins, cell-wall related proteins, protein translation/processing/degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOF MS. The results revealed that of the 21 DEPs in Jimai22 responding to the SRF, 13 DEPs were upregulated and 8 DEPs were downregulated, and that of the 22 DEPs in Luyuan301, 9 DEPs were upregulated and 13 DEPs were downregulated. These DEPs might be responsible for the stronger cold resistance of Jimai22 compared to Luyuan301. The expression pattern and function analysis of these DEPs were very significant to understanding the mechanism of the SRF responses in wheat. |
| first_indexed | 2024-04-12T11:27:45Z |
| format | Article |
| id | doaj.art-b1fbde13a37b4c4a9db6cc5e291d67f7 |
| institution | Directory Open Access Journal |
| issn | 2095-7505 |
| language | English |
| last_indexed | 2024-04-12T11:27:45Z |
| publishDate | 2014-02-01 |
| publisher | Higher Education Press |
| record_format | Article |
| series | Frontiers of Agricultural Science and Engineering |
| spelling | doaj.art-b1fbde13a37b4c4a9db6cc5e291d67f72022-12-22T03:35:10ZengHigher Education PressFrontiers of Agricultural Science and Engineering2095-75052014-02-0111374510.15302/J-FASE-2014008Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frostShujuan ZHANG,Guoqi SONG,Yulian LI,Jie GAO,Jiao WANG,Guiju CHEN,Haosheng LI,Genying LI,Zhendong ZHAO0Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology Genetic Improvement on North Yellow Huai River Valley, Ministry of Agriculture of the People Republic of China; National Engineering Laboratory for Wheat Maize, Jinan 250100, ChinaSpring radiation frost (SRF) is a severe environmental stress which impairs wheat yield and productivity worldwide. To better understand the mechanism of wheat (Triticum aestivum) responding to SRF, a comparative proteomic analysis was performed to analyze the changes of the key proteins in two wheat cultivars Jimai22 and Luyuan301 with high and low tolerance to SRF respectively. A total of 43 differentially expressed proteins (DEPs) which mainly involved in carbohydrate metabolism, amino acid metabolism, resistance proteins and antioxidant enzymes, photosynthesis and cellular respiration proteins, cell-wall related proteins, protein translation/processing/degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOF MS. The results revealed that of the 21 DEPs in Jimai22 responding to the SRF, 13 DEPs were upregulated and 8 DEPs were downregulated, and that of the 22 DEPs in Luyuan301, 9 DEPs were upregulated and 13 DEPs were downregulated. These DEPs might be responsible for the stronger cold resistance of Jimai22 compared to Luyuan301. The expression pattern and function analysis of these DEPs were very significant to understanding the mechanism of the SRF responses in wheat.http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/2095-7505-2014-1-37.pdfcommon wheat|spring radiation frost|proteomic analysis|2-DE|MALDI-TOF-TOF MS |
| spellingShingle | Shujuan ZHANG,Guoqi SONG,Yulian LI,Jie GAO,Jiao WANG,Guiju CHEN,Haosheng LI,Genying LI,Zhendong ZHAO Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost Frontiers of Agricultural Science and Engineering common wheat|spring radiation frost|proteomic analysis|2-DE|MALDI-TOF-TOF MS |
| title | Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost |
| title_full | Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost |
| title_fullStr | Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost |
| title_full_unstemmed | Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost |
| title_short | Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost |
| title_sort | comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost |
| topic | common wheat|spring radiation frost|proteomic analysis|2-DE|MALDI-TOF-TOF MS |
| url | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/2095-7505-2014-1-37.pdf |
| work_keys_str_mv | AT shujuanzhangguoqisongyulianlijiegaojiaowangguijuchenhaoshengligenyinglizhendongzhao comparativeproteomicanalysisofcoldresponsiveproteinsintwowheatcultivarswithdifferenttolerancetospringradiationfrost |