Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing
A variety of modern super-resolution microscopy methods provide researchers with previously inconceivable biological sample imaging opportunities at a molecular resolution. All of these techniques excel at imaging samples that are close to the coverslip, however imaging at large depths remains a cha...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2021-06-01
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| Series: | Nanophotonics |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/nanoph-2021-0108 |
| _version_ | 1819131766290513920 |
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| author | Navikas Vytautas Descloux Adrien C. Grussmayer Kristin S. Marion Sanjin Radenovic Aleksandra |
| author_facet | Navikas Vytautas Descloux Adrien C. Grussmayer Kristin S. Marion Sanjin Radenovic Aleksandra |
| author_sort | Navikas Vytautas |
| collection | DOAJ |
| description | A variety of modern super-resolution microscopy methods provide researchers with previously inconceivable biological sample imaging opportunities at a molecular resolution. All of these techniques excel at imaging samples that are close to the coverslip, however imaging at large depths remains a challenge due to aberrations caused by the sample, diminishing the resolution of the microscope. Originating in astro-imaging, the adaptive optics (AO) approach for wavefront shaping using a deformable mirror is gaining momentum in modern microscopy as a convenient approach for wavefront control. AO has the ability not only to correct aberrations but also enables engineering of the PSF shape, allowing localization of the emitter axial position over several microns. In this study, we demonstrate remote focusing as another AO benefit for super-resolution microscopy. We show the ability to record volumetric data (45 × 45 × 10 µm), while keeping the sample axially stabilized using a standard widefield setup with an adaptive optics addon. We processed the data with single-molecule localization routines and/or computed spatiotemporal correlations, demonstrating subdiffraction resolution. |
| first_indexed | 2024-12-22T09:20:43Z |
| format | Article |
| id | doaj.art-b1fbe6b745644f35b9e804567b223fc1 |
| institution | Directory Open Access Journal |
| issn | 2192-8606 2192-8614 |
| language | English |
| last_indexed | 2024-12-22T09:20:43Z |
| publishDate | 2021-06-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Nanophotonics |
| spelling | doaj.art-b1fbe6b745644f35b9e804567b223fc12022-12-21T18:31:12ZengDe GruyterNanophotonics2192-86062192-86142021-06-011092451245810.1515/nanoph-2021-0108Adaptive optics enables multimode 3D super-resolution microscopy via remote focusingNavikas Vytautas0Descloux Adrien C.1Grussmayer Kristin S.2Marion Sanjin3Radenovic Aleksandra4Laboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, SwitzerlandLaboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, SwitzerlandLaboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, SwitzerlandLaboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, SwitzerlandLaboratory of Nanoscale Biology, Institute of Bioengineering, School of Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, SwitzerlandA variety of modern super-resolution microscopy methods provide researchers with previously inconceivable biological sample imaging opportunities at a molecular resolution. All of these techniques excel at imaging samples that are close to the coverslip, however imaging at large depths remains a challenge due to aberrations caused by the sample, diminishing the resolution of the microscope. Originating in astro-imaging, the adaptive optics (AO) approach for wavefront shaping using a deformable mirror is gaining momentum in modern microscopy as a convenient approach for wavefront control. AO has the ability not only to correct aberrations but also enables engineering of the PSF shape, allowing localization of the emitter axial position over several microns. In this study, we demonstrate remote focusing as another AO benefit for super-resolution microscopy. We show the ability to record volumetric data (45 × 45 × 10 µm), while keeping the sample axially stabilized using a standard widefield setup with an adaptive optics addon. We processed the data with single-molecule localization routines and/or computed spatiotemporal correlations, demonstrating subdiffraction resolution.https://doi.org/10.1515/nanoph-2021-0108adaptive opticsastigmatism-based single molecule localization microscopy3d imagingremote focusingsingle-molecule localization microscopy (smlm)super-resolution optical fluctuation imaging (sofi) |
| spellingShingle | Navikas Vytautas Descloux Adrien C. Grussmayer Kristin S. Marion Sanjin Radenovic Aleksandra Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing Nanophotonics adaptive optics astigmatism-based single molecule localization microscopy 3d imaging remote focusing single-molecule localization microscopy (smlm) super-resolution optical fluctuation imaging (sofi) |
| title | Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing |
| title_full | Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing |
| title_fullStr | Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing |
| title_full_unstemmed | Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing |
| title_short | Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing |
| title_sort | adaptive optics enables multimode 3d super resolution microscopy via remote focusing |
| topic | adaptive optics astigmatism-based single molecule localization microscopy 3d imaging remote focusing single-molecule localization microscopy (smlm) super-resolution optical fluctuation imaging (sofi) |
| url | https://doi.org/10.1515/nanoph-2021-0108 |
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