Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis
Abstract Background The conventional belief that glucocorticosteroid (GC) acts through a single brand glucocorticoid receptor (GR)α protein has changed dramatically with the discovery of multiple GR isoforms. We aimed to evaluate whether multiple GR protein isoforms are expressed in chronic rhinosin...
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| Format: | Article |
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BMC
2022-06-01
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| Series: | Journal of Otolaryngology - Head and Neck Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40463-022-00561-1 |
| _version_ | 1797970197691236352 |
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| author | Shan Shao Yue Wang Yan Zhao Yuan Xu Tie Wang Kun Du Shiping Bao Xiangdong Wang Luo Zhang |
| author_facet | Shan Shao Yue Wang Yan Zhao Yuan Xu Tie Wang Kun Du Shiping Bao Xiangdong Wang Luo Zhang |
| author_sort | Shan Shao |
| collection | DOAJ |
| description | Abstract Background The conventional belief that glucocorticosteroid (GC) acts through a single brand glucocorticoid receptor (GR)α protein has changed dramatically with the discovery of multiple GR isoforms. We aimed to evaluate whether multiple GR protein isoforms are expressed in chronic rhinosinusitis with nasal polyps (CRSwNP) and whether GR protein isoform expression profiles differ between different endotypes of CRSwNP. Methods Thirty-eight patients with CRSwNP and ten healthy volunteers were included. The protein expression of multiple GR isoforms in nasal polyps (NPs) tissue and control mucosae was examined by western blot analysis with different GR antibodies. Results Five bands, including three bands for known proteins (GRα-A/B, GRα-C, and GRα-D) and two bands for unidentified proteins at 67 kilodaltons (kDa) and 60 kDa, were identified with both total GR antibody (PA1-511A) and GRα-specific antibody (PA1-516). GRα-D intensity, which was abundant in nasal mucosa, was significantly increased in the CRSwNP group and was especially elevated in the noneosinophilic CRSwNP (NE-CRSwNP) group (PA1-511A: P < 0.001 and P = 0.0018; PA1-516: P < 0.003 and P = 0.006, respectively). Additionally, the intensities of the newly recognized 67 kDa and 60 kDa bands were much greater in the NE-CRSwNP subgroup than in the eosinophilic CRSwNP (E-CRSwNP) subgroup; in the E-CRSwNP subgroup, the median intensities were even lower than those in the control group. Conclusions This study provides evidence that nasal tissues express multiple GR protein isoforms. GR protein isoforms presented disease and tissue-specific expression profiles that differed between the CRSwNP and control groups and between the E-CRSwNP and NE-CRSwNP subgroups. Graphical abstract |
| first_indexed | 2024-04-11T03:13:01Z |
| format | Article |
| id | doaj.art-b1fcaf6871cc4124a1175706cf94fa4a |
| institution | Directory Open Access Journal |
| issn | 1916-0216 |
| language | English |
| last_indexed | 2024-04-11T03:13:01Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Otolaryngology - Head and Neck Surgery |
| spelling | doaj.art-b1fcaf6871cc4124a1175706cf94fa4a2023-01-02T11:04:31ZengBMCJournal of Otolaryngology - Head and Neck Surgery1916-02162022-06-0151111110.1186/s40463-022-00561-1Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitisShan Shao0Yue Wang1Yan Zhao2Yuan Xu3Tie Wang4Kun Du5Shiping Bao6Xiangdong Wang7Luo Zhang8Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical UniversityDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical UniversityBeijing Key Laboratory of Nasal Diseases, Beijing Institute of OtolaryngologyDepartments of Oncology, Community Health Sciences, and Surgery, Cumming School of Medicine, and The Center for Health Informatics, University of CalgaryMIID Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of CalgaryDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical UniversityDepartment of Otolaryngology Head and Neck Surgery, Beijing Youan Hospital, Capital Medical UniversityDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical UniversityDepartment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical UniversityAbstract Background The conventional belief that glucocorticosteroid (GC) acts through a single brand glucocorticoid receptor (GR)α protein has changed dramatically with the discovery of multiple GR isoforms. We aimed to evaluate whether multiple GR protein isoforms are expressed in chronic rhinosinusitis with nasal polyps (CRSwNP) and whether GR protein isoform expression profiles differ between different endotypes of CRSwNP. Methods Thirty-eight patients with CRSwNP and ten healthy volunteers were included. The protein expression of multiple GR isoforms in nasal polyps (NPs) tissue and control mucosae was examined by western blot analysis with different GR antibodies. Results Five bands, including three bands for known proteins (GRα-A/B, GRα-C, and GRα-D) and two bands for unidentified proteins at 67 kilodaltons (kDa) and 60 kDa, were identified with both total GR antibody (PA1-511A) and GRα-specific antibody (PA1-516). GRα-D intensity, which was abundant in nasal mucosa, was significantly increased in the CRSwNP group and was especially elevated in the noneosinophilic CRSwNP (NE-CRSwNP) group (PA1-511A: P < 0.001 and P = 0.0018; PA1-516: P < 0.003 and P = 0.006, respectively). Additionally, the intensities of the newly recognized 67 kDa and 60 kDa bands were much greater in the NE-CRSwNP subgroup than in the eosinophilic CRSwNP (E-CRSwNP) subgroup; in the E-CRSwNP subgroup, the median intensities were even lower than those in the control group. Conclusions This study provides evidence that nasal tissues express multiple GR protein isoforms. GR protein isoforms presented disease and tissue-specific expression profiles that differed between the CRSwNP and control groups and between the E-CRSwNP and NE-CRSwNP subgroups. Graphical abstracthttps://doi.org/10.1186/s40463-022-00561-1Nasal polypsSinusitisGlucocorticoid receptorEndotypes |
| spellingShingle | Shan Shao Yue Wang Yan Zhao Yuan Xu Tie Wang Kun Du Shiping Bao Xiangdong Wang Luo Zhang Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis Journal of Otolaryngology - Head and Neck Surgery Nasal polyps Sinusitis Glucocorticoid receptor Endotypes |
| title | Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis |
| title_full | Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis |
| title_fullStr | Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis |
| title_full_unstemmed | Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis |
| title_short | Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis |
| title_sort | identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis |
| topic | Nasal polyps Sinusitis Glucocorticoid receptor Endotypes |
| url | https://doi.org/10.1186/s40463-022-00561-1 |
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