Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradation
The concentration of free cytosolic Ca2+ is a critical second messenger in almost every cell type, with the signal often being carried by the period of oscillations, or spikes, in the cytosolic Ca2+ concentration. We have previously studied how Ca2+ influx across the plasma membrane affects the peri...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Systems Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsysb.2024.1343006/full |
| _version_ | 1797261796011147264 |
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| author | Clara Octors Ryan E. Yoast Scott M. Emrich Mohamed Trebak Mohamed Trebak Mohamed Trebak James Sneyd |
| author_facet | Clara Octors Ryan E. Yoast Scott M. Emrich Mohamed Trebak Mohamed Trebak Mohamed Trebak James Sneyd |
| author_sort | Clara Octors |
| collection | DOAJ |
| description | The concentration of free cytosolic Ca2+ is a critical second messenger in almost every cell type, with the signal often being carried by the period of oscillations, or spikes, in the cytosolic Ca2+ concentration. We have previously studied how Ca2+ influx across the plasma membrane affects the period and shape of Ca2+ oscillations in HEK293 cells. However, our theoretical work was unable to explain how the shape of Ca2+ oscillations could change qualitatively, from thin spikes to broad oscillations, during the course of a single time series. Such qualitative changes in oscillation shape are a common feature of HEK293 cells in which STIM1 and 2 have been knocked out. Here, we present an extended version of our earlier model that suggests that such time-dependent qualitative changes in oscillation shape might be the result of balanced positive and negative feedback from Ca2+ to the production and degradation of inositol trisphosphate. |
| first_indexed | 2024-04-24T23:46:54Z |
| format | Article |
| id | doaj.art-b202a630471c4ed18986bdee9a74b97d |
| institution | Directory Open Access Journal |
| issn | 2674-0702 |
| language | English |
| last_indexed | 2024-04-24T23:46:54Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Systems Biology |
| spelling | doaj.art-b202a630471c4ed18986bdee9a74b97d2024-03-15T04:53:48ZengFrontiers Media S.A.Frontiers in Systems Biology2674-07022024-03-01410.3389/fsysb.2024.13430061343006Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradationClara Octors0Ryan E. Yoast1Scott M. Emrich2Mohamed Trebak3Mohamed Trebak4Mohamed Trebak5James Sneyd6Department of Mathematical Biology and Physical Chemistry, Science Faculty, Université Libre de Bruxelles (ULB), Brussels, BelgiumGraduate Program in Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, United StatesGraduate Program in Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey, PA, United StatesDepartment of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesVascular Medicine Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesUPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesDepartment of Mathematics, University of Auckland, Auckland, New ZealandThe concentration of free cytosolic Ca2+ is a critical second messenger in almost every cell type, with the signal often being carried by the period of oscillations, or spikes, in the cytosolic Ca2+ concentration. We have previously studied how Ca2+ influx across the plasma membrane affects the period and shape of Ca2+ oscillations in HEK293 cells. However, our theoretical work was unable to explain how the shape of Ca2+ oscillations could change qualitatively, from thin spikes to broad oscillations, during the course of a single time series. Such qualitative changes in oscillation shape are a common feature of HEK293 cells in which STIM1 and 2 have been knocked out. Here, we present an extended version of our earlier model that suggests that such time-dependent qualitative changes in oscillation shape might be the result of balanced positive and negative feedback from Ca2+ to the production and degradation of inositol trisphosphate.https://www.frontiersin.org/articles/10.3389/fsysb.2024.1343006/fullcalcium oscillationcalcium influxHEK293 cellsSOCEPLC regulationIP3 |
| spellingShingle | Clara Octors Ryan E. Yoast Scott M. Emrich Mohamed Trebak Mohamed Trebak Mohamed Trebak James Sneyd Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradation Frontiers in Systems Biology calcium oscillation calcium influx HEK293 cells SOCE PLC regulation IP3 |
| title | Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradation |
| title_full | Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradation |
| title_fullStr | Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradation |
| title_full_unstemmed | Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradation |
| title_short | Calcium oscillations in HEK293 cells lacking SOCE suggest the existence of a balanced regulation of IP3 production and degradation |
| title_sort | calcium oscillations in hek293 cells lacking soce suggest the existence of a balanced regulation of ip3 production and degradation |
| topic | calcium oscillation calcium influx HEK293 cells SOCE PLC regulation IP3 |
| url | https://www.frontiersin.org/articles/10.3389/fsysb.2024.1343006/full |
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