Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction
Coffee has been a common ingredient in many traditional hair loss remedies, but limited scientific evidence supports its use, particularly in coffee pulp. Androgenetic alopecia (AGA) is caused by androgens, inflammation, and oxidative stress. In the present study, supercritical fluid extraction (SFE...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/22/4116 |
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| author | Anurak Muangsanguan Pichchapa Linsaenkart Tanakarn Chaitep Jiraporn Sangta Sarana Rose Sommano Korawan Sringarm Chaiwat Arjin Pornchai Rachtanapun Kittisak Jantanasakulwong Yuthana Phimolsiripol Juan M. Castagnini Warintorn Ruksiriwanich |
| author_facet | Anurak Muangsanguan Pichchapa Linsaenkart Tanakarn Chaitep Jiraporn Sangta Sarana Rose Sommano Korawan Sringarm Chaiwat Arjin Pornchai Rachtanapun Kittisak Jantanasakulwong Yuthana Phimolsiripol Juan M. Castagnini Warintorn Ruksiriwanich |
| author_sort | Anurak Muangsanguan |
| collection | DOAJ |
| description | Coffee has been a common ingredient in many traditional hair loss remedies, but limited scientific evidence supports its use, particularly in coffee pulp. Androgenetic alopecia (AGA) is caused by androgens, inflammation, and oxidative stress. In the present study, supercritical fluid extraction (SFE) was used under various conditions to obtain six coffee pulp extracts. The SFE-4 extract, using 50% (<i>v</i>/v) ethanol as a co-solvent at conditions of 100 °C and 500 bars for 30 min, exhibited the highest phenolic, flavonoid, and caffeine contents. Additionally, the SFE-4 extract increased the migration and cell proliferation of HFDPCs (human hair follicle dermal papilla cells), which control hair cycle regulation, and had scavenging effects on ABTS and DPPH radicals. Additionally, the SFE-4 extract showed potassium ion channel opener activity in HFDPCs, as well as a stimulation effect on the enzyme matrix metalloproteinase-2 (MMP-2) (28.53 ± 1.08% of control), which may be related to the vascular endothelial growth factor (<i>VEGF</i>) gene upregulation. In human prostate cancer cells (DU-145) and HFDPC cells, the SFE-4 extract significantly decreased the expression of <i>SRD5A1</i>, <i>SRD5A2</i>, and <i>SRD5A3</i>, an essential pathway involved in AGA. Hair growth factor genes in the Wnt/-catenin (<i>CTNNB1</i>) and Sonic Hedgehog (<i>SHH</i>, <i>SMO</i>, and <i>GLI1</i>) pathways could be significantly activated by the SFE-4 extract. These results imply that employing SFE in coffee pulp extraction could help AGA treatment by preventing hair loss and promoting hair growth pathways. This would help small coffee producers gain economic empowerment and ensure the long-term sustainability of agricultural waste utilization. |
| first_indexed | 2024-03-09T16:50:07Z |
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| id | doaj.art-b2034a59ae6b427e8b2aa5f823fa36ef |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T16:50:07Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj.art-b2034a59ae6b427e8b2aa5f823fa36ef2023-11-24T14:42:07ZengMDPI AGFoods2304-81582023-11-011222411610.3390/foods12224116Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid ExtractionAnurak Muangsanguan0Pichchapa Linsaenkart1Tanakarn Chaitep2Jiraporn Sangta3Sarana Rose Sommano4Korawan Sringarm5Chaiwat Arjin6Pornchai Rachtanapun7Kittisak Jantanasakulwong8Yuthana Phimolsiripol9Juan M. Castagnini10Warintorn Ruksiriwanich11Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandInterdisciplinary Program in Biotechnology, Graduate School, Chiang Mai University, Chiang Mai 50200, ThailandCluster of Valorization and Bio-Green Transformation for Translation Research Innovation of Raw Materials and Products, Chiang Mai University, Chiang Mai 50200, ThailandCluster of Valorization and Bio-Green Transformation for Translation Research Innovation of Raw Materials and Products, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandCenter of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandCenter of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandCenter of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandResearch Group in Innovative Technologies for Sustainable Food (ALISOST), Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy, Universitat de València, Avenida Vicent Andrés Estellés s/n, 46100 Burjassot, SpainDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandCoffee has been a common ingredient in many traditional hair loss remedies, but limited scientific evidence supports its use, particularly in coffee pulp. Androgenetic alopecia (AGA) is caused by androgens, inflammation, and oxidative stress. In the present study, supercritical fluid extraction (SFE) was used under various conditions to obtain six coffee pulp extracts. The SFE-4 extract, using 50% (<i>v</i>/v) ethanol as a co-solvent at conditions of 100 °C and 500 bars for 30 min, exhibited the highest phenolic, flavonoid, and caffeine contents. Additionally, the SFE-4 extract increased the migration and cell proliferation of HFDPCs (human hair follicle dermal papilla cells), which control hair cycle regulation, and had scavenging effects on ABTS and DPPH radicals. Additionally, the SFE-4 extract showed potassium ion channel opener activity in HFDPCs, as well as a stimulation effect on the enzyme matrix metalloproteinase-2 (MMP-2) (28.53 ± 1.08% of control), which may be related to the vascular endothelial growth factor (<i>VEGF</i>) gene upregulation. In human prostate cancer cells (DU-145) and HFDPC cells, the SFE-4 extract significantly decreased the expression of <i>SRD5A1</i>, <i>SRD5A2</i>, and <i>SRD5A3</i>, an essential pathway involved in AGA. Hair growth factor genes in the Wnt/-catenin (<i>CTNNB1</i>) and Sonic Hedgehog (<i>SHH</i>, <i>SMO</i>, and <i>GLI1</i>) pathways could be significantly activated by the SFE-4 extract. These results imply that employing SFE in coffee pulp extraction could help AGA treatment by preventing hair loss and promoting hair growth pathways. This would help small coffee producers gain economic empowerment and ensure the long-term sustainability of agricultural waste utilization.https://www.mdpi.com/2304-8158/12/22/4116androgenetic alopecia<i>Coffea arabica</i>hair loss treatmentSonic HedgehogWnt/β-cateninwaste utilization |
| spellingShingle | Anurak Muangsanguan Pichchapa Linsaenkart Tanakarn Chaitep Jiraporn Sangta Sarana Rose Sommano Korawan Sringarm Chaiwat Arjin Pornchai Rachtanapun Kittisak Jantanasakulwong Yuthana Phimolsiripol Juan M. Castagnini Warintorn Ruksiriwanich Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction Foods androgenetic alopecia <i>Coffea arabica</i> hair loss treatment Sonic Hedgehog Wnt/β-catenin waste utilization |
| title | Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction |
| title_full | Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction |
| title_fullStr | Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction |
| title_full_unstemmed | Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction |
| title_short | Hair Growth Promotion and Anti-Hair Loss Effects of By-Products Arabica Coffee Pulp Extracts Using Supercritical Fluid Extraction |
| title_sort | hair growth promotion and anti hair loss effects of by products arabica coffee pulp extracts using supercritical fluid extraction |
| topic | androgenetic alopecia <i>Coffea arabica</i> hair loss treatment Sonic Hedgehog Wnt/β-catenin waste utilization |
| url | https://www.mdpi.com/2304-8158/12/22/4116 |
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