Transcriptome analysis of indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.).
Tea (Camellia sinensis L.) is a popular world beverage, and propagation of tea plants chiefly depends on the formation of adventitious roots in cuttings. To better understand potential mechanisms involved in adventitious root formation, we performed transcriptome analysis of single nodal cuttings of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4162609?pdf=render |
| _version_ | 1819087732659453952 |
|---|---|
| author | Kang Wei Li-Yuan Wang Li-Yun Wu Cheng-Cai Zhang Hai-Lin Li Li-Qiang Tan Hong-Li Cao Hao Cheng |
| author_facet | Kang Wei Li-Yuan Wang Li-Yun Wu Cheng-Cai Zhang Hai-Lin Li Li-Qiang Tan Hong-Li Cao Hao Cheng |
| author_sort | Kang Wei |
| collection | DOAJ |
| description | Tea (Camellia sinensis L.) is a popular world beverage, and propagation of tea plants chiefly depends on the formation of adventitious roots in cuttings. To better understand potential mechanisms involved in adventitious root formation, we performed transcriptome analysis of single nodal cuttings of C. sinensis treated with or without indole-3-butyric acid (IBA) using the Illumina sequencing method. Totally 42.5 million RNA-Seq reads were obtained and these were assembled into 59,931 unigenes, with an average length of 732 bp and an N50 of 1292 bp. In addition, 1091 differentially expressed unigenes were identified in the tea cuttings treated with IBA compared to controls, including 656 up- and 435 down-regulated genes. Further real time RT-PCR analysis confirmed RNA-Seq data. Functional annotation analysis showed that many genes were involved in plant hormone signal transduction, secondary metabolism, cell wall organization and glutathione metabolism, indicating potential contributions to adventitious rooting. Our study presents a global view of transcriptome profiles of tea cuttings in response to IBA treatment and provides new insights into the fundamental mechanisms associated with auxin-induced adventitious rooting. Our data will be a valuable resource for genomic research about adventitious root formation in tea cuttings, which can be used to improve rooting for difficult-to-root varieties. |
| first_indexed | 2024-12-21T21:40:49Z |
| format | Article |
| id | doaj.art-b2041438b1ae4f80a379f898c7357c1e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T21:40:49Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b2041438b1ae4f80a379f898c7357c1e2022-12-21T18:49:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10720110.1371/journal.pone.0107201Transcriptome analysis of indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.).Kang WeiLi-Yuan WangLi-Yun WuCheng-Cai ZhangHai-Lin LiLi-Qiang TanHong-Li CaoHao ChengTea (Camellia sinensis L.) is a popular world beverage, and propagation of tea plants chiefly depends on the formation of adventitious roots in cuttings. To better understand potential mechanisms involved in adventitious root formation, we performed transcriptome analysis of single nodal cuttings of C. sinensis treated with or without indole-3-butyric acid (IBA) using the Illumina sequencing method. Totally 42.5 million RNA-Seq reads were obtained and these were assembled into 59,931 unigenes, with an average length of 732 bp and an N50 of 1292 bp. In addition, 1091 differentially expressed unigenes were identified in the tea cuttings treated with IBA compared to controls, including 656 up- and 435 down-regulated genes. Further real time RT-PCR analysis confirmed RNA-Seq data. Functional annotation analysis showed that many genes were involved in plant hormone signal transduction, secondary metabolism, cell wall organization and glutathione metabolism, indicating potential contributions to adventitious rooting. Our study presents a global view of transcriptome profiles of tea cuttings in response to IBA treatment and provides new insights into the fundamental mechanisms associated with auxin-induced adventitious rooting. Our data will be a valuable resource for genomic research about adventitious root formation in tea cuttings, which can be used to improve rooting for difficult-to-root varieties.http://europepmc.org/articles/PMC4162609?pdf=render |
| spellingShingle | Kang Wei Li-Yuan Wang Li-Yun Wu Cheng-Cai Zhang Hai-Lin Li Li-Qiang Tan Hong-Li Cao Hao Cheng Transcriptome analysis of indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.). PLoS ONE |
| title | Transcriptome analysis of indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.). |
| title_full | Transcriptome analysis of indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.). |
| title_fullStr | Transcriptome analysis of indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.). |
| title_full_unstemmed | Transcriptome analysis of indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.). |
| title_short | Transcriptome analysis of indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.). |
| title_sort | transcriptome analysis of indole 3 butyric acid induced adventitious root formation in nodal cuttings of camellia sinensis l |
| url | http://europepmc.org/articles/PMC4162609?pdf=render |
| work_keys_str_mv | AT kangwei transcriptomeanalysisofindole3butyricacidinducedadventitiousrootformationinnodalcuttingsofcamelliasinensisl AT liyuanwang transcriptomeanalysisofindole3butyricacidinducedadventitiousrootformationinnodalcuttingsofcamelliasinensisl AT liyunwu transcriptomeanalysisofindole3butyricacidinducedadventitiousrootformationinnodalcuttingsofcamelliasinensisl AT chengcaizhang transcriptomeanalysisofindole3butyricacidinducedadventitiousrootformationinnodalcuttingsofcamelliasinensisl AT hailinli transcriptomeanalysisofindole3butyricacidinducedadventitiousrootformationinnodalcuttingsofcamelliasinensisl AT liqiangtan transcriptomeanalysisofindole3butyricacidinducedadventitiousrootformationinnodalcuttingsofcamelliasinensisl AT honglicao transcriptomeanalysisofindole3butyricacidinducedadventitiousrootformationinnodalcuttingsofcamelliasinensisl AT haocheng transcriptomeanalysisofindole3butyricacidinducedadventitiousrootformationinnodalcuttingsofcamelliasinensisl |