Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice
SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic. The virus spreads rapidly with a high transmission rate among humans, and hence virus management has been challenging owing to finding specific therapies or vaccinations. Hence, an effective, low-cost vaccine is urgently required. In this...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/11/1961 |
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| author | Chalisa Panapitakkul Narach Khorattanakulchai Kaewta Rattanapisit Theerakarn Srisangsung Balamurugan Shanmugaraj Supranee Buranapraditkun Chutitorn Ketloy Eakachai Prompetchara Waranyoo Phoolcharoen |
| author_facet | Chalisa Panapitakkul Narach Khorattanakulchai Kaewta Rattanapisit Theerakarn Srisangsung Balamurugan Shanmugaraj Supranee Buranapraditkun Chutitorn Ketloy Eakachai Prompetchara Waranyoo Phoolcharoen |
| author_sort | Chalisa Panapitakkul |
| collection | DOAJ |
| description | SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic. The virus spreads rapidly with a high transmission rate among humans, and hence virus management has been challenging owing to finding specific therapies or vaccinations. Hence, an effective, low-cost vaccine is urgently required. In this study, the immunogenicity of the plant-produced S1 subunit protein of SARS-CoV-2 was examined in order to assess it as a potential candidate for SARS-CoV-2. The SARS-CoV-2 S1-Fc fusion protein was transiently produced in <i>Nicotiana benthamiana</i>. Within four days of infiltration, the SARS-CoV-2 S1-Fc protein was expressed in high quantities, and using protein A affinity column chromatography, plant-produced S1-Fc protein was purified from the crude extracts. The characterization of plant-produced S1-Fc protein was analyzed by SDS-PAGE and Western blotting. Immunogenicity of the purified S1-Fc protein formulated with alum induced both RBD specific antibodies and T cell immune responses in mice. These preliminary results indicated that the plant-produced S1 protein is immunogenic in mice. |
| first_indexed | 2024-03-09T17:56:28Z |
| format | Article |
| id | doaj.art-b206196ec6ec4ab184ca683ed14d08ab |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T17:56:28Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-b206196ec6ec4ab184ca683ed14d08ab2023-11-24T10:17:19ZengMDPI AGVaccines2076-393X2022-11-011011196110.3390/vaccines10111961Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in MiceChalisa Panapitakkul0Narach Khorattanakulchai1Kaewta Rattanapisit2Theerakarn Srisangsung3Balamurugan Shanmugaraj4Supranee Buranapraditkun5Chutitorn Ketloy6Eakachai Prompetchara7Waranyoo Phoolcharoen8Center of Excellence in Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok 10330, ThailandCenter of Excellence in Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok 10330, ThailandBaiya Phytopharm Co., Ltd., Bangkok 10330, ThailandBaiya Phytopharm Co., Ltd., Bangkok 10330, ThailandBaiya Phytopharm Co., Ltd., Bangkok 10330, ThailandCenter of Excellence in Vaccine Research and Development (Chula VRC), Chulalongkorn University, Bangkok 10330, ThailandCenter of Excellence in Vaccine Research and Development (Chula VRC), Chulalongkorn University, Bangkok 10330, ThailandCenter of Excellence in Vaccine Research and Development (Chula VRC), Chulalongkorn University, Bangkok 10330, ThailandCenter of Excellence in Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok 10330, ThailandSARS-CoV-2 is responsible for the ongoing COVID-19 pandemic. The virus spreads rapidly with a high transmission rate among humans, and hence virus management has been challenging owing to finding specific therapies or vaccinations. Hence, an effective, low-cost vaccine is urgently required. In this study, the immunogenicity of the plant-produced S1 subunit protein of SARS-CoV-2 was examined in order to assess it as a potential candidate for SARS-CoV-2. The SARS-CoV-2 S1-Fc fusion protein was transiently produced in <i>Nicotiana benthamiana</i>. Within four days of infiltration, the SARS-CoV-2 S1-Fc protein was expressed in high quantities, and using protein A affinity column chromatography, plant-produced S1-Fc protein was purified from the crude extracts. The characterization of plant-produced S1-Fc protein was analyzed by SDS-PAGE and Western blotting. Immunogenicity of the purified S1-Fc protein formulated with alum induced both RBD specific antibodies and T cell immune responses in mice. These preliminary results indicated that the plant-produced S1 protein is immunogenic in mice.https://www.mdpi.com/2076-393X/10/11/1961SARS-CoV-2subunit vaccinespike proteinplant-produced recombinant protein |
| spellingShingle | Chalisa Panapitakkul Narach Khorattanakulchai Kaewta Rattanapisit Theerakarn Srisangsung Balamurugan Shanmugaraj Supranee Buranapraditkun Chutitorn Ketloy Eakachai Prompetchara Waranyoo Phoolcharoen Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice Vaccines SARS-CoV-2 subunit vaccine spike protein plant-produced recombinant protein |
| title | Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice |
| title_full | Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice |
| title_fullStr | Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice |
| title_full_unstemmed | Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice |
| title_short | Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice |
| title_sort | plant produced s1 subunit protein of sars cov 2 elicits immunogenic responses in mice |
| topic | SARS-CoV-2 subunit vaccine spike protein plant-produced recombinant protein |
| url | https://www.mdpi.com/2076-393X/10/11/1961 |
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