Rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactation
Dietary fatty acid (FA) composition in early postnatal life can modulate growth and development and later metabolic health. Investigating programming effects of early dietary FA manipulations in rodents may be stressful and complicated due to the need of artificial feeding techniques. It is largely...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2015-01-01
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| Series: | Journal of Nutritional Science |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2048679015000130/type/journal_article |
| _version_ | 1811156013701660672 |
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| author | Annemarie Oosting Henkjan J. Verkade Diane Kegler Bert J. M. van de Heijning Eline M. van der Beek |
| author_facet | Annemarie Oosting Henkjan J. Verkade Diane Kegler Bert J. M. van de Heijning Eline M. van der Beek |
| author_sort | Annemarie Oosting |
| collection | DOAJ |
| description | Dietary fatty acid (FA) composition in early postnatal life can modulate growth and development and later metabolic health. Investigating programming effects of early dietary FA manipulations in rodents may be stressful and complicated due to the need of artificial feeding techniques. It is largely unknown to what extent breast milk (BM) FA composition can be directly manipulated by the diet. We exposed dams to different dietary FA compositions from postnatal day (PN) 2 until PN28. Dams with litters were randomly assigned to control (CTRL), high-medium-chain FA (MCFA), low-linoleic acid (LowLA), high-n-3 long-chain PUFA (n-3LCP) or high-n-3LCP and MCFA (n-3LCP/MCFA) diets, and diets were continued after weaning until PN28. FA compositions were determined in feeds, milk and in erythrocytes. BM MCFA content was independent from dietary MCFA intake. In contrast, the LowLA diet reduced BM LA content by about 50 % compared with the CTRL diet at PN7. BM of dams fed the n-3LCP or n-3LCP/MCFA diet contained about 6-fold more n-3 LCP than BM of the dams fed the CTRL diet at PN7. These changes in milk FA composition established after 5 d of dietary exposure did not further change over the lactation period. At PN28, the erythrocyte FA composition of the male pups correlated with analysed milk FA profiles. In conclusion, manipulation of the diet of lactating mice can strongly and rapidly affect BM FA composition, in particular of n-6 LA and n-3 LCP. Our present findings will facilitate mechanistic studies on the programming of adult metabolic health by dietary FA in the early postnatal period via direct and selective manipulation of the maternal diet. |
| first_indexed | 2024-04-10T04:44:15Z |
| format | Article |
| id | doaj.art-b20861295c334df7acae05de66203c65 |
| institution | Directory Open Access Journal |
| issn | 2048-6790 |
| language | English |
| last_indexed | 2024-04-10T04:44:15Z |
| publishDate | 2015-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Nutritional Science |
| spelling | doaj.art-b20861295c334df7acae05de66203c652023-03-09T12:38:43ZengCambridge University PressJournal of Nutritional Science2048-67902015-01-01410.1017/jns.2015.13Rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactationAnnemarie Oosting0Henkjan J. Verkade1Diane Kegler2Bert J. M. van de Heijning3Eline M. van der Beek4Nutricia Research, Utrecht, The NetherlandsDepartment of Pediatric Gastroenterology and Hepatology, Beatrix Children's Hospital - University Medical Center Groningen, University of Groningen, Groningen, The NetherlandsNutricia Research, Utrecht, The NetherlandsNutricia Research, Utrecht, The NetherlandsNutricia Research, Singapore 138671, SingaporeDietary fatty acid (FA) composition in early postnatal life can modulate growth and development and later metabolic health. Investigating programming effects of early dietary FA manipulations in rodents may be stressful and complicated due to the need of artificial feeding techniques. It is largely unknown to what extent breast milk (BM) FA composition can be directly manipulated by the diet. We exposed dams to different dietary FA compositions from postnatal day (PN) 2 until PN28. Dams with litters were randomly assigned to control (CTRL), high-medium-chain FA (MCFA), low-linoleic acid (LowLA), high-n-3 long-chain PUFA (n-3LCP) or high-n-3LCP and MCFA (n-3LCP/MCFA) diets, and diets were continued after weaning until PN28. FA compositions were determined in feeds, milk and in erythrocytes. BM MCFA content was independent from dietary MCFA intake. In contrast, the LowLA diet reduced BM LA content by about 50 % compared with the CTRL diet at PN7. BM of dams fed the n-3LCP or n-3LCP/MCFA diet contained about 6-fold more n-3 LCP than BM of the dams fed the CTRL diet at PN7. These changes in milk FA composition established after 5 d of dietary exposure did not further change over the lactation period. At PN28, the erythrocyte FA composition of the male pups correlated with analysed milk FA profiles. In conclusion, manipulation of the diet of lactating mice can strongly and rapidly affect BM FA composition, in particular of n-6 LA and n-3 LCP. Our present findings will facilitate mechanistic studies on the programming of adult metabolic health by dietary FA in the early postnatal period via direct and selective manipulation of the maternal diet.https://www.cambridge.org/core/product/identifier/S2048679015000130/type/journal_articleDietary fat qualityMilk fatty acid compositionMouse models |
| spellingShingle | Annemarie Oosting Henkjan J. Verkade Diane Kegler Bert J. M. van de Heijning Eline M. van der Beek Rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactation Journal of Nutritional Science Dietary fat quality Milk fatty acid composition Mouse models |
| title | Rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactation |
| title_full | Rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactation |
| title_fullStr | Rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactation |
| title_full_unstemmed | Rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactation |
| title_short | Rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactation |
| title_sort | rapid and selective manipulation of milk fatty acid composition in mice through the maternal diet during lactation |
| topic | Dietary fat quality Milk fatty acid composition Mouse models |
| url | https://www.cambridge.org/core/product/identifier/S2048679015000130/type/journal_article |
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