Molecular analysis of Cryptosporidium species in domestic goat in central Iraq
<em>Cryptosporidium</em> spp. is a significant parasitic disease that results in diarrhea and gastroenteritis in humans and animals worldwide. The present study aimed to investigate the molecular diversity of <em>Cryptosporidium</em> species in domestic goats. A total of a hu...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2022-10-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_174693_81561e5ad18e2bba4524323e5965031e.pdf |
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| author | Akeel M. Al-Musawi Abdul Hussein H. Awad Mansoor J. Alkhaled |
| author_facet | Akeel M. Al-Musawi Abdul Hussein H. Awad Mansoor J. Alkhaled |
| author_sort | Akeel M. Al-Musawi |
| collection | DOAJ |
| description | <em>Cryptosporidium</em> spp. is a significant parasitic disease that results in diarrhea and gastroenteritis in humans and animals worldwide. The present study aimed to investigate the molecular diversity of <em>Cryptosporidium</em> species in domestic goats. A total of a hundred feces samples were collected from four locations in Babylon city in central Iraq. All the samples were investigated phenotypically using a modified Ziehl-Neelsen stain method and genotypically using conventional and nested PCR methods based on a partial sequence of heat shock protein 70 (Hsp70) and 60 kDa glycoprotein (gp60) genes, and finally, phylogenetic analysis method. The molecular results showed five species of <em>Cryptosporidium</em>, including <em>C. parvum, C. hominis, C. ryana, C. xiaoi, </em>and<em> C. bovis</em>. The phylogenetic results of partial sequence of gp60 gene for <em>C. parvum</em> and C. hominis isolate two subtypes were established IIdA21G1 and IIdA19G1 belong to <em>C. parvum</em>. For <em>C. hominis</em>, three subtypes were detected: IbA21G2, IbA13G3, and IbA19G2. This study showed that <em>Cryptosporidium parvum </em>(zoonotic) is more prevalent than other <em>Cryptosporidium</em> species in goats from this area. This suggests that zoonotic transmission is the primary mode of transmission of <em>Cryptosporidium</em> infection in Babylon province. |
| first_indexed | 2024-04-11T17:07:13Z |
| format | Article |
| id | doaj.art-b20e3ada1da24dffba8a91aa64102884 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-11T17:07:13Z |
| publishDate | 2022-10-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-b20e3ada1da24dffba8a91aa641028842022-12-22T04:13:00ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552022-10-013641041104510.33899/ijvs.2022.132974.2155174693Molecular analysis of Cryptosporidium species in domestic goat in central IraqAkeel M. Al-Musawi0Abdul Hussein H. Awad1Mansoor J. Alkhaled2Department of Parasitology, College of Veterinary Medicine, Al-Qasim Green University, Babylon, IraqDepartment of Biology, College of Education for Pure Sciences, Basrah University, Basrah, IraqDepartment of Parasitology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq<em>Cryptosporidium</em> spp. is a significant parasitic disease that results in diarrhea and gastroenteritis in humans and animals worldwide. The present study aimed to investigate the molecular diversity of <em>Cryptosporidium</em> species in domestic goats. A total of a hundred feces samples were collected from four locations in Babylon city in central Iraq. All the samples were investigated phenotypically using a modified Ziehl-Neelsen stain method and genotypically using conventional and nested PCR methods based on a partial sequence of heat shock protein 70 (Hsp70) and 60 kDa glycoprotein (gp60) genes, and finally, phylogenetic analysis method. The molecular results showed five species of <em>Cryptosporidium</em>, including <em>C. parvum, C. hominis, C. ryana, C. xiaoi, </em>and<em> C. bovis</em>. The phylogenetic results of partial sequence of gp60 gene for <em>C. parvum</em> and C. hominis isolate two subtypes were established IIdA21G1 and IIdA19G1 belong to <em>C. parvum</em>. For <em>C. hominis</em>, three subtypes were detected: IbA21G2, IbA13G3, and IbA19G2. This study showed that <em>Cryptosporidium parvum </em>(zoonotic) is more prevalent than other <em>Cryptosporidium</em> species in goats from this area. This suggests that zoonotic transmission is the primary mode of transmission of <em>Cryptosporidium</em> infection in Babylon province.https://www.vetmedmosul.com/article_174693_81561e5ad18e2bba4524323e5965031e.pdfcryptosporidium sppgenotypesubtypesdomestic goatiraq |
| spellingShingle | Akeel M. Al-Musawi Abdul Hussein H. Awad Mansoor J. Alkhaled Molecular analysis of Cryptosporidium species in domestic goat in central Iraq Iraqi Journal of Veterinary Sciences cryptosporidium spp genotype subtypes domestic goat iraq |
| title | Molecular analysis of Cryptosporidium species in domestic goat in central Iraq |
| title_full | Molecular analysis of Cryptosporidium species in domestic goat in central Iraq |
| title_fullStr | Molecular analysis of Cryptosporidium species in domestic goat in central Iraq |
| title_full_unstemmed | Molecular analysis of Cryptosporidium species in domestic goat in central Iraq |
| title_short | Molecular analysis of Cryptosporidium species in domestic goat in central Iraq |
| title_sort | molecular analysis of cryptosporidium species in domestic goat in central iraq |
| topic | cryptosporidium spp genotype subtypes domestic goat iraq |
| url | https://www.vetmedmosul.com/article_174693_81561e5ad18e2bba4524323e5965031e.pdf |
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