Chemoprotective Effect of <i>Plantago sempervirens</i> Crantz Extract on Ovarian Structure and Folliculogenesis
Alkylating agents such as cyclophosphamide (CPA) are commonly used in cytotoxic or immunosuppressive therapies for different types of diseases. One of the main secondary effects of such therapy is the reduction in follicular reserve, targeting the primordial follicles. The aim of this study was to i...
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MDPI AG
2023-02-01
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| author | Anca D. Stoica Bogdan Sevastre Maria Suciu Alina Elena Pârvu Marcel Pârvu Vlad Alexandru Toma Ioana Roman Camelia Dobre |
| author_facet | Anca D. Stoica Bogdan Sevastre Maria Suciu Alina Elena Pârvu Marcel Pârvu Vlad Alexandru Toma Ioana Roman Camelia Dobre |
| author_sort | Anca D. Stoica |
| collection | DOAJ |
| description | Alkylating agents such as cyclophosphamide (CPA) are commonly used in cytotoxic or immunosuppressive therapies for different types of diseases. One of the main secondary effects of such therapy is the reduction in follicular reserve, targeting the primordial follicles. The aim of this study was to investigate the antioxidant and protective effects of <i>Plantago sempervirens</i> extract on the follicular pool. The experiment was performed on Wistar female rats, for 21 days. They were divided into five groups according to the treatment they received (Control, CPA (200 mg/kg BW), <i>P. sempervirens</i> extract in three different doses—25/50/100 dw/mL ethanol extract (5 mL/kg BW)). The investigations regarded enzymatic and non-enzymatic nitro-oxidative stress, hormone levels (FSH and estrogen), TEM sections of the ovaries, and oestrus cycle monitorization. CPA strongly increased the oxidative stress (TOS—32.8 mmol H<sub>2</sub>O<sub>2</sub>/L; NO—68.60 μmol/L; OSI—6.82), alongside a depletion of FSH and a blockage of the oestrus cycle in the metestrus phase. The follicular pool was strongly depleted during CPA exposure, but FSH concentration and the TEM images of the cells revealed a significant improvement after treatment, as the dose increases (P3). Therefore, these findings reveal that <i>P. sempervirens</i> therapy could have a potential protective effect against CPA-induced acute damages in the ovaries, as well as maintaining a functional oestrus cycle. |
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| last_indexed | 2024-03-11T07:31:00Z |
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| spelling | doaj.art-b20eaf13ac7944278ead1ae9e1041ed82023-11-17T07:19:38ZengMDPI AGApplied Sciences2076-34172023-02-01135313410.3390/app13053134Chemoprotective Effect of <i>Plantago sempervirens</i> Crantz Extract on Ovarian Structure and FolliculogenesisAnca D. Stoica0Bogdan Sevastre1Maria Suciu2Alina Elena Pârvu3Marcel Pârvu4Vlad Alexandru Toma5Ioana Roman6Camelia Dobre7Faculty of Biology and Geology, Babeș-Bolyai University, 400006 Cluj-Napoca, RomaniaFaculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaFaculty of Biology and Geology, Babeș-Bolyai University, 400006 Cluj-Napoca, RomaniaFaculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400347 Cluj-Napoca, RomaniaFaculty of Biology and Geology, Babeș-Bolyai University, 400006 Cluj-Napoca, RomaniaFaculty of Biology and Geology, Babeș-Bolyai University, 400006 Cluj-Napoca, RomaniaInstitute of Biological Research, Branch of NIRDBS Bucharest, 400015 Cluj-Napoca, RomaniaFaculty of Biology and Geology, Babeș-Bolyai University, 400006 Cluj-Napoca, RomaniaAlkylating agents such as cyclophosphamide (CPA) are commonly used in cytotoxic or immunosuppressive therapies for different types of diseases. One of the main secondary effects of such therapy is the reduction in follicular reserve, targeting the primordial follicles. The aim of this study was to investigate the antioxidant and protective effects of <i>Plantago sempervirens</i> extract on the follicular pool. The experiment was performed on Wistar female rats, for 21 days. They were divided into five groups according to the treatment they received (Control, CPA (200 mg/kg BW), <i>P. sempervirens</i> extract in three different doses—25/50/100 dw/mL ethanol extract (5 mL/kg BW)). The investigations regarded enzymatic and non-enzymatic nitro-oxidative stress, hormone levels (FSH and estrogen), TEM sections of the ovaries, and oestrus cycle monitorization. CPA strongly increased the oxidative stress (TOS—32.8 mmol H<sub>2</sub>O<sub>2</sub>/L; NO—68.60 μmol/L; OSI—6.82), alongside a depletion of FSH and a blockage of the oestrus cycle in the metestrus phase. The follicular pool was strongly depleted during CPA exposure, but FSH concentration and the TEM images of the cells revealed a significant improvement after treatment, as the dose increases (P3). Therefore, these findings reveal that <i>P. sempervirens</i> therapy could have a potential protective effect against CPA-induced acute damages in the ovaries, as well as maintaining a functional oestrus cycle.https://www.mdpi.com/2076-3417/13/5/3134oxidative stresscyclophosphamideoestrus cycle<i>Plantago sempervirens</i> |
| spellingShingle | Anca D. Stoica Bogdan Sevastre Maria Suciu Alina Elena Pârvu Marcel Pârvu Vlad Alexandru Toma Ioana Roman Camelia Dobre Chemoprotective Effect of <i>Plantago sempervirens</i> Crantz Extract on Ovarian Structure and Folliculogenesis Applied Sciences oxidative stress cyclophosphamide oestrus cycle <i>Plantago sempervirens</i> |
| title | Chemoprotective Effect of <i>Plantago sempervirens</i> Crantz Extract on Ovarian Structure and Folliculogenesis |
| title_full | Chemoprotective Effect of <i>Plantago sempervirens</i> Crantz Extract on Ovarian Structure and Folliculogenesis |
| title_fullStr | Chemoprotective Effect of <i>Plantago sempervirens</i> Crantz Extract on Ovarian Structure and Folliculogenesis |
| title_full_unstemmed | Chemoprotective Effect of <i>Plantago sempervirens</i> Crantz Extract on Ovarian Structure and Folliculogenesis |
| title_short | Chemoprotective Effect of <i>Plantago sempervirens</i> Crantz Extract on Ovarian Structure and Folliculogenesis |
| title_sort | chemoprotective effect of i plantago sempervirens i crantz extract on ovarian structure and folliculogenesis |
| topic | oxidative stress cyclophosphamide oestrus cycle <i>Plantago sempervirens</i> |
| url | https://www.mdpi.com/2076-3417/13/5/3134 |
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