Transcriptional and Metabolic Response of Wine-Related <i>Lactiplantibacillus plantarum</i> to Different Conditions of Aeration and Nitrogen Availability
Lactic acid bacteria (LAB) perform the process of malolactic fermentation (MLF) in wine. Availability of oxygen and nitrogen nutrients could influence LAB growth, malolactic activity, and other metabolic pathways, impacting the subsequent wine quality. The impact of these two factors has received li...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/2/68 |
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| author | Renato L. Binati Maret Du Toit Jacky L. Snoep Elisa Salvetti Sandra Torriani |
| author_facet | Renato L. Binati Maret Du Toit Jacky L. Snoep Elisa Salvetti Sandra Torriani |
| author_sort | Renato L. Binati |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) perform the process of malolactic fermentation (MLF) in wine. Availability of oxygen and nitrogen nutrients could influence LAB growth, malolactic activity, and other metabolic pathways, impacting the subsequent wine quality. The impact of these two factors has received limited investigation within LAB, especially on a transcriptome level. The aim of this study was to evaluate metabolic changes in the strain <i>Lactiplantibacillus plantarum</i> IWBT B063, growing in synthetic grape juice medium (GJM) under different oxygen exposure conditions, and with low availability of nitrogen-based nutrients. Next-generation sequencing was used to analyze expression across the transcriptome (RNA-seq), in combination with conventional microbiological and chemical analysis. <i>L. plantarum</i> consumed the malic acid present in all the conditions evaluated, with a slight delay and impaired growth for nitrogen limitation and for anaerobiosis. Comparison of <i>L. plantarum</i> transcriptome during growth in GJM with and without O<sub>2</sub> revealed differential expression of 148 functionally annotated genes, which were mostly involved in carbohydrate metabolism, genetic information processing, and signaling and cellular processes. In particular, genes with a protective role against oxidative stress and genes related to amino acid metabolism were differentially expressed. This study confirms the suitability of <i>L. plantarum</i> IWBT B063 to carry out MLF in different environmental conditions due to its potential adaption to the stress conditions tested and provides a better understanding of the genetic background of an industrially relevant strain. |
| first_indexed | 2024-03-10T11:49:27Z |
| format | Article |
| id | doaj.art-b21272ca0cd549a2b994aac75165061d |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T11:49:27Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-b21272ca0cd549a2b994aac75165061d2023-11-21T17:49:56ZengMDPI AGFermentation2311-56372021-04-01726810.3390/fermentation7020068Transcriptional and Metabolic Response of Wine-Related <i>Lactiplantibacillus plantarum</i> to Different Conditions of Aeration and Nitrogen AvailabilityRenato L. Binati0Maret Du Toit1Jacky L. Snoep2Elisa Salvetti3Sandra Torriani4Department of Biotechnology, University of Verona, Strada Le Grazie 15, Ca’ Vignal 2, 37134 Verona, ItalySouth African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University, Private Bag X1, 7602 Matieland, South AfricaDepartment of Biochemistry, Stellenbosch University, Private Bag X1, 7602 Matieland, South AfricaDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, Ca’ Vignal 2, 37134 Verona, ItalyDepartment of Biotechnology, University of Verona, Strada Le Grazie 15, Ca’ Vignal 2, 37134 Verona, ItalyLactic acid bacteria (LAB) perform the process of malolactic fermentation (MLF) in wine. Availability of oxygen and nitrogen nutrients could influence LAB growth, malolactic activity, and other metabolic pathways, impacting the subsequent wine quality. The impact of these two factors has received limited investigation within LAB, especially on a transcriptome level. The aim of this study was to evaluate metabolic changes in the strain <i>Lactiplantibacillus plantarum</i> IWBT B063, growing in synthetic grape juice medium (GJM) under different oxygen exposure conditions, and with low availability of nitrogen-based nutrients. Next-generation sequencing was used to analyze expression across the transcriptome (RNA-seq), in combination with conventional microbiological and chemical analysis. <i>L. plantarum</i> consumed the malic acid present in all the conditions evaluated, with a slight delay and impaired growth for nitrogen limitation and for anaerobiosis. Comparison of <i>L. plantarum</i> transcriptome during growth in GJM with and without O<sub>2</sub> revealed differential expression of 148 functionally annotated genes, which were mostly involved in carbohydrate metabolism, genetic information processing, and signaling and cellular processes. In particular, genes with a protective role against oxidative stress and genes related to amino acid metabolism were differentially expressed. This study confirms the suitability of <i>L. plantarum</i> IWBT B063 to carry out MLF in different environmental conditions due to its potential adaption to the stress conditions tested and provides a better understanding of the genetic background of an industrially relevant strain.https://www.mdpi.com/2311-5637/7/2/68malolactic fermentation<i>L. plantarum</i>anaerobic growthnutrient availabilityRNA-seq |
| spellingShingle | Renato L. Binati Maret Du Toit Jacky L. Snoep Elisa Salvetti Sandra Torriani Transcriptional and Metabolic Response of Wine-Related <i>Lactiplantibacillus plantarum</i> to Different Conditions of Aeration and Nitrogen Availability Fermentation malolactic fermentation <i>L. plantarum</i> anaerobic growth nutrient availability RNA-seq |
| title | Transcriptional and Metabolic Response of Wine-Related <i>Lactiplantibacillus plantarum</i> to Different Conditions of Aeration and Nitrogen Availability |
| title_full | Transcriptional and Metabolic Response of Wine-Related <i>Lactiplantibacillus plantarum</i> to Different Conditions of Aeration and Nitrogen Availability |
| title_fullStr | Transcriptional and Metabolic Response of Wine-Related <i>Lactiplantibacillus plantarum</i> to Different Conditions of Aeration and Nitrogen Availability |
| title_full_unstemmed | Transcriptional and Metabolic Response of Wine-Related <i>Lactiplantibacillus plantarum</i> to Different Conditions of Aeration and Nitrogen Availability |
| title_short | Transcriptional and Metabolic Response of Wine-Related <i>Lactiplantibacillus plantarum</i> to Different Conditions of Aeration and Nitrogen Availability |
| title_sort | transcriptional and metabolic response of wine related i lactiplantibacillus plantarum i to different conditions of aeration and nitrogen availability |
| topic | malolactic fermentation <i>L. plantarum</i> anaerobic growth nutrient availability RNA-seq |
| url | https://www.mdpi.com/2311-5637/7/2/68 |
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