In-vitro Differentiation of Human Umbilical Cord Wharton’s Jelly Mesenchymal Stem Cells to Insulin-Producing Cells
Background & Objective: Diabetes is a major chronic metabolic disease in the world. Islet transplantation is a way to treat diabetes. Unfortunately, this method is restricted due to graft rejection and lack of donor islets. Mesenchymal Stem Cells (MSCS) have the ability to differentiate into Ins...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Fasa University of Medical Sciences
2015-05-01
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| Series: | Journal of Fasa University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.fums.ac.ir/browse.php?a_code=A-10-120-1&slc_lang=en&sid=1 |
| Summary: | Background &
Objective: Diabetes is a major chronic metabolic disease in the world. Islet
transplantation is a way to treat diabetes. Unfortunately, this method is
restricted due to graft rejection and lack of donor islets. Mesenchymal Stem Cells (MSCS) have the ability
to differentiate into Insulin-Producing Cells (IPCs). In this study, Human
Umbilical Mesenchymal Stem Cells (HUMSCS) were induced to
differentiate into pancreatic β-like
cells. Materials &
Methods: The samples were collected after cesarean section delivery at
Hafez hospital. HUMSCS were cultured in sterile condition, in three
steps for 20 days in DMEM-F12, Retinoic Acid (RA), Epidermal Growth
Factor (EGF), exendin-4, Fetal Bovine Serum (FBS), and antibiotic. Then, they
were differentiated into IPC. DTZ staining employed for determining the
presence of insulin and Reveres Transcription- Polymerase
Chain Reaction (RT-PCR) was done for identifying of gene expression including
insulin, PDX1, and NGN3. The Insulin concentration was also evaluated by
Immunoradiometric assay. Results: HUMSCS
gradually changed from fibroblast-shaped cells to epithelial-like cells and
eventually to IPC under special conditions. RT-PCR experiments revealed that
these cells expressed insulin, PDX1, and NGN3 genes. The
cells became red color when stained with DTZ and the insulin secretion was
confirmed. Conclusion: HUMSCS
have the ability to differentiate into islet-like cells in vitro and may
be a new potential source for cell transplantation in diabetes treatment. |
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| ISSN: | 2228-5105 2228-7329 |