Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation
Objective: To study ovarian gross morphologic and subanatomic features across pubertal development. Design: Prospective cohort study. Setting: An academic medical center with specimens collected from 2018–2022. Patient(s): Tissue was obtained from prepubertal and postpubertal participants (0.19–22.9...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | F&S Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666334123000168 |
| _version_ | 1797802736110010368 |
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| author | Elizabeth L. Tsui, B.S. Courtney J. Harris, M.D. Erin E. Rowell, M.D. Monica M. Laronda, Ph.D. |
| author_facet | Elizabeth L. Tsui, B.S. Courtney J. Harris, M.D. Erin E. Rowell, M.D. Monica M. Laronda, Ph.D. |
| author_sort | Elizabeth L. Tsui, B.S. |
| collection | DOAJ |
| description | Objective: To study ovarian gross morphologic and subanatomic features across pubertal development. Design: Prospective cohort study. Setting: An academic medical center with specimens collected from 2018–2022. Patient(s): Tissue was obtained from prepubertal and postpubertal participants (0.19–22.96 years) undergoing ovarian tissue cryopreservation before treatment that put them at a significantly or high increased risk of developing premature ovarian insufficiency. Most participants (64%) had not received chemotherapy at tissue collection. Intervention(s): None. Main Outcome Measure(s): Ovaries procured for fertility preservation were weighed and measured. Ovarian tissue fragments released during processing, biopsies used for pathology, and hormone panels were analyzed for gross morphology, subanatomic features, and reproductive hormones. Graphical analysis of best-fit lines determined age at maximum growth velocity. Result(s): Prepubertal ovaries were significantly (1.4-fold and 2.4-fold) smaller than postpubertal ovaries by length and width and 5.7-fold lighter on average. Length, width, and weight grew in a sigmoidal pattern with age. Prepubertal ovaries were less likely to display a defined corticomedullary junction (53% vs. 77% in postpubertal specimens), less likely to have a tunica albuginea (22% vs. 93% in postpubertal specimens), contained significantly more (9.8-fold) primordial follicles, and contained primordial follicles at significantly deeper depths (2.9-fold) when compared with postpubertal ovaries. Conclusion(s): Ovarian tissue cryopreservation is a resource to study human ovarian biology and pubertal development. Maximum growth velocity occurs late within the pubertal transition (Tanner 3+) after changes in subanatomic features. This ovarian morphology model adds to foundational knowledge of human ovarian development and supports ongoing transcriptomics research. |
| first_indexed | 2024-03-13T05:10:14Z |
| format | Article |
| id | doaj.art-b216ae7b3c4a4497b03de6ca286795b5 |
| institution | Directory Open Access Journal |
| issn | 2666-3341 |
| language | English |
| last_indexed | 2024-03-13T05:10:14Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | F&S Reports |
| spelling | doaj.art-b216ae7b3c4a4497b03de6ca286795b52023-06-16T05:11:47ZengElsevierF&S Reports2666-33412023-06-0142196205Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservationElizabeth L. Tsui, B.S.0Courtney J. Harris, M.D.1Erin E. Rowell, M.D.2Monica M. Laronda, Ph.D.3Stanley Manne Children’s Research Institute, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois; Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IllinoisDepartment of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois; Division of Pediatric Surgery, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, IllinoisDepartment of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois; Division of Pediatric Surgery, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, IllinoisStanley Manne Children’s Research Institute, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois; Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, Illinois; Division of Pediatric Surgery, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois; Reprint requests: Monica M. Laronda, Ph.D., Stanley Manne Children’s Research Institute, 225 E Chicago Avenue, Box 205, Chicago, IL 60611.Objective: To study ovarian gross morphologic and subanatomic features across pubertal development. Design: Prospective cohort study. Setting: An academic medical center with specimens collected from 2018–2022. Patient(s): Tissue was obtained from prepubertal and postpubertal participants (0.19–22.96 years) undergoing ovarian tissue cryopreservation before treatment that put them at a significantly or high increased risk of developing premature ovarian insufficiency. Most participants (64%) had not received chemotherapy at tissue collection. Intervention(s): None. Main Outcome Measure(s): Ovaries procured for fertility preservation were weighed and measured. Ovarian tissue fragments released during processing, biopsies used for pathology, and hormone panels were analyzed for gross morphology, subanatomic features, and reproductive hormones. Graphical analysis of best-fit lines determined age at maximum growth velocity. Result(s): Prepubertal ovaries were significantly (1.4-fold and 2.4-fold) smaller than postpubertal ovaries by length and width and 5.7-fold lighter on average. Length, width, and weight grew in a sigmoidal pattern with age. Prepubertal ovaries were less likely to display a defined corticomedullary junction (53% vs. 77% in postpubertal specimens), less likely to have a tunica albuginea (22% vs. 93% in postpubertal specimens), contained significantly more (9.8-fold) primordial follicles, and contained primordial follicles at significantly deeper depths (2.9-fold) when compared with postpubertal ovaries. Conclusion(s): Ovarian tissue cryopreservation is a resource to study human ovarian biology and pubertal development. Maximum growth velocity occurs late within the pubertal transition (Tanner 3+) after changes in subanatomic features. This ovarian morphology model adds to foundational knowledge of human ovarian development and supports ongoing transcriptomics research.http://www.sciencedirect.com/science/article/pii/S2666334123000168Pediatric ovariesovarian tissue cryopreservationovarycorticomedullary junctionovarian biology |
| spellingShingle | Elizabeth L. Tsui, B.S. Courtney J. Harris, M.D. Erin E. Rowell, M.D. Monica M. Laronda, Ph.D. Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation F&S Reports Pediatric ovaries ovarian tissue cryopreservation ovary corticomedullary junction ovarian biology |
| title | Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation |
| title_full | Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation |
| title_fullStr | Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation |
| title_full_unstemmed | Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation |
| title_short | Human ovarian gross morphology and subanatomy across puberty: insights from tissue donated during fertility preservation |
| title_sort | human ovarian gross morphology and subanatomy across puberty insights from tissue donated during fertility preservation |
| topic | Pediatric ovaries ovarian tissue cryopreservation ovary corticomedullary junction ovarian biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666334123000168 |
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