THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTS
The aim of the work was to study the effect of introns of the rice OsAct1 and the maize hsp70 genes on the transgene expression in Nicotiana plants in order to find out of their use in the testing of vectors containing these monocot introns. Next methods were used: Agrobacterium-mediated transformat...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2018-08-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/4_2018/nitovska_4_2018.pdf |
| _version_ | 1827884629633269760 |
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| author | I. O. Nitovska M. Yu. Vasylenko B. V. Morgun |
| author_facet | I. O. Nitovska M. Yu. Vasylenko B. V. Morgun |
| author_sort | I. O. Nitovska |
| collection | DOAJ |
| description | The aim of the work was to study the effect of introns of the rice OsAct1 and the maize hsp70 genes on the transgene expression in Nicotiana plants in order to find out of their use in the testing of vectors containing these monocot introns. Next methods were used: Agrobacterium-mediated transformation of leaves of greenhouse N. benthamiana and N. tabacum plants by vector pCB271 containing the introns of cereals, light fluorescence microscopy and fluorimetry of GFP. The presence of transgenes was detected by polymerase chain reaction. The transient GFP expression was observed in infiltrated tissue of N. benthamiana. Transgenic plants of N. tabacum resistant to kanamycin were obtained. Fluorescence of GFP in extracts of some transgenic tobacco lines was shown. The impairment of the transgene expression in some N. tabacum transformants has been observed. So, transgenes, containing introns from the hsp70 corn or from the OsAct1 rice genes downstream the promotor, are expressed in Nicotiana plants. Thus, N. benthamiana and N. Tabacum plants can be used to test vectors constructs for cereals transformation. It has been shown that the monocot introns can have the negative impact on the transgene expression in Nicotiana plants. |
| first_indexed | 2024-03-12T19:32:49Z |
| format | Article |
| id | doaj.art-b219ac766ffe41d781687249954a5fb4 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T19:32:49Z |
| publishDate | 2018-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-b219ac766ffe41d781687249954a5fb42023-08-02T04:24:43ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2018-08-01114738310.15407/biotech11.04.073THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTSI. O. Nitovska0M. Yu. Vasylenko1B. V. Morgun2Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, KiyvInstitute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, KiyvInstitute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, KiyvThe aim of the work was to study the effect of introns of the rice OsAct1 and the maize hsp70 genes on the transgene expression in Nicotiana plants in order to find out of their use in the testing of vectors containing these monocot introns. Next methods were used: Agrobacterium-mediated transformation of leaves of greenhouse N. benthamiana and N. tabacum plants by vector pCB271 containing the introns of cereals, light fluorescence microscopy and fluorimetry of GFP. The presence of transgenes was detected by polymerase chain reaction. The transient GFP expression was observed in infiltrated tissue of N. benthamiana. Transgenic plants of N. tabacum resistant to kanamycin were obtained. Fluorescence of GFP in extracts of some transgenic tobacco lines was shown. The impairment of the transgene expression in some N. tabacum transformants has been observed. So, transgenes, containing introns from the hsp70 corn or from the OsAct1 rice genes downstream the promotor, are expressed in Nicotiana plants. Thus, N. benthamiana and N. Tabacum plants can be used to test vectors constructs for cereals transformation. It has been shown that the monocot introns can have the negative impact on the transgene expression in Nicotiana plants.http://biotechnology.kiev.ua/images/storage/4_2018/nitovska_4_2018.pdfmonocot intronsNicotianaAgrobacterium-mediated transformationGFP |
| spellingShingle | I. O. Nitovska M. Yu. Vasylenko B. V. Morgun THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTS Biotechnologia Acta monocot introns Nicotiana Agrobacterium-mediated transformation GFP |
| title | THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTS |
| title_full | THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTS |
| title_fullStr | THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTS |
| title_full_unstemmed | THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTS |
| title_short | THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTS |
| title_sort | effect of monocot introns on transgene expression in nicotiana genus plants |
| topic | monocot introns Nicotiana Agrobacterium-mediated transformation GFP |
| url | http://biotechnology.kiev.ua/images/storage/4_2018/nitovska_4_2018.pdf |
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