Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya
We describe a novel virus, designated Ntepes virus (NPV), isolated from sand flies in Kenya. NPV has the characteristic phlebovirus trisegmented genome architecture and is related to, but distinct from, Gabek Forest phlebovirus. Diverse cell cultures derived from wildlife, livestock, and humans were...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Centers for Disease Control and Prevention
2019-04-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/25/4/18-0750_article |
| _version_ | 1828521005415399424 |
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| author | David P. Tchouassi Marco Marklewitz Edith Chepkorir Florian Zirkel Sheila B. Agha Caroline C. Tigoi Edith Koskei Christian Drosten Christian Borgemeister Baldwyn Torto Sandra Junglen Rosemary Sang |
| author_facet | David P. Tchouassi Marco Marklewitz Edith Chepkorir Florian Zirkel Sheila B. Agha Caroline C. Tigoi Edith Koskei Christian Drosten Christian Borgemeister Baldwyn Torto Sandra Junglen Rosemary Sang |
| author_sort | David P. Tchouassi |
| collection | DOAJ |
| description | We describe a novel virus, designated Ntepes virus (NPV), isolated from sand flies in Kenya. NPV has the characteristic phlebovirus trisegmented genome architecture and is related to, but distinct from, Gabek Forest phlebovirus. Diverse cell cultures derived from wildlife, livestock, and humans were susceptible to NPV, with pronounced permissiveness in swine and rodent cells. NPV infection of newborn mice caused rapid and fatal illness. Permissiveness for NPV replication in sand fly cells, but not mosquito cells, suggests a vector-specific adaptation. Specific neutralizing antibodies were found in 13.9% (26/187) of human serum samples taken at the site of isolation of NPV as well as a disparate site in northeastern Kenya, suggesting a wide distribution. We identify a novel human-infecting arbovirus and highlight the importance of rural areas in tropical Africa for arbovirus surveillance as well as extending arbovirus surveillance to include hematophagous arthropods other than mosquitoes. |
| first_indexed | 2024-12-11T19:41:07Z |
| format | Article |
| id | doaj.art-b21ae79b2f94408296c97d9427d9a047 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-11T19:41:07Z |
| publishDate | 2019-04-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-b21ae79b2f94408296c97d9427d9a0472022-12-22T00:53:01ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592019-04-0125468169010.3201/eid2504.180750Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, KenyaDavid P. TchouassiMarco MarklewitzEdith ChepkorirFlorian ZirkelSheila B. AghaCaroline C. TigoiEdith KoskeiChristian DrostenChristian BorgemeisterBaldwyn TortoSandra JunglenRosemary SangWe describe a novel virus, designated Ntepes virus (NPV), isolated from sand flies in Kenya. NPV has the characteristic phlebovirus trisegmented genome architecture and is related to, but distinct from, Gabek Forest phlebovirus. Diverse cell cultures derived from wildlife, livestock, and humans were susceptible to NPV, with pronounced permissiveness in swine and rodent cells. NPV infection of newborn mice caused rapid and fatal illness. Permissiveness for NPV replication in sand fly cells, but not mosquito cells, suggests a vector-specific adaptation. Specific neutralizing antibodies were found in 13.9% (26/187) of human serum samples taken at the site of isolation of NPV as well as a disparate site in northeastern Kenya, suggesting a wide distribution. We identify a novel human-infecting arbovirus and highlight the importance of rural areas in tropical Africa for arbovirus surveillance as well as extending arbovirus surveillance to include hematophagous arthropods other than mosquitoes.https://wwwnc.cdc.gov/eid/article/25/4/18-0750_articlearbovirusphlebovirussandfliesvirus discoveryKenyaviruses |
| spellingShingle | David P. Tchouassi Marco Marklewitz Edith Chepkorir Florian Zirkel Sheila B. Agha Caroline C. Tigoi Edith Koskei Christian Drosten Christian Borgemeister Baldwyn Torto Sandra Junglen Rosemary Sang Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya Emerging Infectious Diseases arbovirus phlebovirus sandflies virus discovery Kenya viruses |
| title | Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya |
| title_full | Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya |
| title_fullStr | Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya |
| title_full_unstemmed | Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya |
| title_short | Sand Fly–Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya |
| title_sort | sand fly associated phlebovirus with evidence of neutralizing antibodies in humans kenya |
| topic | arbovirus phlebovirus sandflies virus discovery Kenya viruses |
| url | https://wwwnc.cdc.gov/eid/article/25/4/18-0750_article |
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