Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from Romania
“Poor responders” (PR) are an important category of infertile women who experience a modest response to controlled ovarian stimulation. In this study, we evaluated response to growth hormone (GH) administration among PR patient subtypes stratified by follicle stimulation hormone receptor (FSHR) poly...
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MDPI AG
2022-09-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/10/2371 |
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| author | Mihai Surcel Bogdan Doroftei Iulia Adina Neamtiu Daniel Muresan Gabriela Caracostea Iulian Goidescu Adelina Staicu Georgiana Nemeti Michael S. Bloom Cristina Zlatescu-Marton |
| author_facet | Mihai Surcel Bogdan Doroftei Iulia Adina Neamtiu Daniel Muresan Gabriela Caracostea Iulian Goidescu Adelina Staicu Georgiana Nemeti Michael S. Bloom Cristina Zlatescu-Marton |
| author_sort | Mihai Surcel |
| collection | DOAJ |
| description | “Poor responders” (PR) are an important category of infertile women who experience a modest response to controlled ovarian stimulation. In this study, we evaluated response to growth hormone (GH) administration among PR patient subtypes stratified by follicle stimulation hormone receptor (FSHR) polymorphism (c.2039A > G p.Asn680Ser). We conducted a cohort study of 125 women with poor ovarian response, 58 of whom received GH in addition to the standard treatment, and 67 of whom received the standard treatment only. The Ala307Thr polymorphism genotypes were analyzed using a polymerase chain reaction-restriction fragment length polymorphism method, and the FSHR gene polymorphism was analyzed using a predesigned TaqMan SNP Genotyping Assay (rs6166). A comparative analysis detected statistically significant differences in mean mature follicles (<i>p</i> = 0.0002), metaphase-II oocytes (<i>p</i> = 0.0005), progesterone levels (<i>p</i> = 0.0036), and IGF levels (follicle IGF1, <i>p</i> = 0.0004) between GH-treated and non-GH-treated participants with the FSHR (Ser/Ser) polymorphism. However, the differences were modest among participants with the other two FSHR polymorphisms (Ser/Asn and Asn/Asn). The subcategory of patients with the FSHR Asn680Ser (Ser/Ser) polymorphism showed a stronger response when GH was added to the IVF protocol. |
| first_indexed | 2024-03-09T20:22:36Z |
| format | Article |
| id | doaj.art-b21c01603aa54792ab6f379075acc585 |
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| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T20:22:36Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-b21c01603aa54792ab6f379075acc5852023-11-23T23:44:02ZengMDPI AGDiagnostics2075-44182022-09-011210237110.3390/diagnostics12102371Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from RomaniaMihai Surcel0Bogdan Doroftei1Iulia Adina Neamtiu2Daniel Muresan3Gabriela Caracostea4Iulian Goidescu5Adelina Staicu6Georgiana Nemeti7Michael S. Bloom8Cristina Zlatescu-Marton91st Department of Obstetrics and Gynecology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 3-5 Clinicilor Street, 400006 Cluj-Napoca, RomaniaFaculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaHealth Department, Environmental Health Center, 58 Busuiocului Street, 400240 Cluj-Napoca, Romania1st Department of Obstetrics and Gynecology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 3-5 Clinicilor Street, 400006 Cluj-Napoca, Romania1st Department of Obstetrics and Gynecology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 3-5 Clinicilor Street, 400006 Cluj-Napoca, Romania1st Department of Obstetrics and Gynecology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 3-5 Clinicilor Street, 400006 Cluj-Napoca, Romania1st Department of Obstetrics and Gynecology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 3-5 Clinicilor Street, 400006 Cluj-Napoca, Romania1st Department of Obstetrics and Gynecology, “Iuliu Hatieganu” University of Medicine and Pharmacy, 3-5 Clinicilor Street, 400006 Cluj-Napoca, RomaniaDepartment of Global and Community Health, George Mason University, 4400 University Drive, MS 5B7, Fairfax, VA 22030, USA“Regina Maria” Hospital, 29 Dorobantilor Street, 400117 Cluj-Napoca, Romania“Poor responders” (PR) are an important category of infertile women who experience a modest response to controlled ovarian stimulation. In this study, we evaluated response to growth hormone (GH) administration among PR patient subtypes stratified by follicle stimulation hormone receptor (FSHR) polymorphism (c.2039A > G p.Asn680Ser). We conducted a cohort study of 125 women with poor ovarian response, 58 of whom received GH in addition to the standard treatment, and 67 of whom received the standard treatment only. The Ala307Thr polymorphism genotypes were analyzed using a polymerase chain reaction-restriction fragment length polymorphism method, and the FSHR gene polymorphism was analyzed using a predesigned TaqMan SNP Genotyping Assay (rs6166). A comparative analysis detected statistically significant differences in mean mature follicles (<i>p</i> = 0.0002), metaphase-II oocytes (<i>p</i> = 0.0005), progesterone levels (<i>p</i> = 0.0036), and IGF levels (follicle IGF1, <i>p</i> = 0.0004) between GH-treated and non-GH-treated participants with the FSHR (Ser/Ser) polymorphism. However, the differences were modest among participants with the other two FSHR polymorphisms (Ser/Asn and Asn/Asn). The subcategory of patients with the FSHR Asn680Ser (Ser/Ser) polymorphism showed a stronger response when GH was added to the IVF protocol.https://www.mdpi.com/2075-4418/12/10/2371growth hormoneinfertility disorderin vitro fertilizationpolymorphismRomaniawomen |
| spellingShingle | Mihai Surcel Bogdan Doroftei Iulia Adina Neamtiu Daniel Muresan Gabriela Caracostea Iulian Goidescu Adelina Staicu Georgiana Nemeti Michael S. Bloom Cristina Zlatescu-Marton Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from Romania Diagnostics growth hormone infertility disorder in vitro fertilization polymorphism Romania women |
| title | Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from Romania |
| title_full | Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from Romania |
| title_fullStr | Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from Romania |
| title_full_unstemmed | Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from Romania |
| title_short | Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from Romania |
| title_sort | impact of follicle stimulating hormone receptor fshr polymorphism on the efficiency of co treatment with growth hormone in a group of infertile women from romania |
| topic | growth hormone infertility disorder in vitro fertilization polymorphism Romania women |
| url | https://www.mdpi.com/2075-4418/12/10/2371 |
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