Sequential Extraction of Valuable Bio-Products from Snow Crab (<i>Chionoecetes opilio</i>) Processing Discards Using Eco-Friendly Methods
Green extraction methods using a combination of mechanical, enzymatic, and green chemical treatments were evaluated for the sequential extraction of carotenoid pigments, protein, and chitin from crab processing discards. Key objectives included avoiding the use of hazardous chemical solvents, conduc...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/21/6/366 |
| _version_ | 1797593737010872320 |
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| author | Heather J. Burke Francesca Kerton |
| author_facet | Heather J. Burke Francesca Kerton |
| author_sort | Heather J. Burke |
| collection | DOAJ |
| description | Green extraction methods using a combination of mechanical, enzymatic, and green chemical treatments were evaluated for the sequential extraction of carotenoid pigments, protein, and chitin from crab processing discards. Key objectives included avoiding the use of hazardous chemical solvents, conducting as close to a 100% green extraction as possible, and developing simple processes to facilitate implementation into processing plants without the need for complicated and expensive equipment. Three crab bio-products were obtained: pigmented vegetable oil, pigmented protein powder, and chitin. Carotenoid extractions were performed using vegetable oils (corn, canola, and sunflower oils), giving between 24.85% and 37.93% astaxanthin recovery. Citric acid was used to demineralize the remaining material and afforded a pigmented protein powder. Three different proteases were used to deproteinate and isolate chitin in yields between 17.06% and 19.15%. The chitin was still highly colored and therefore decolorization was attempted using hydrogen peroxide. Characterization studies were conducted on each of the crab bio-products isolated including powder X-ray diffraction analysis on the chitin (80.18% crystallinity index, CI, achieved using green methods). Overall, three valuable bio-products could be obtained but further research is needed to obtain pigment-free chitin in an environmentally friendly manner. |
| first_indexed | 2024-03-11T02:13:43Z |
| format | Article |
| id | doaj.art-b21c1c6527f14187b2a1f979e4251d32 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T02:13:43Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-b21c1c6527f14187b2a1f979e4251d322023-11-18T11:22:53ZengMDPI AGMarine Drugs1660-33972023-06-0121636610.3390/md21060366Sequential Extraction of Valuable Bio-Products from Snow Crab (<i>Chionoecetes opilio</i>) Processing Discards Using Eco-Friendly MethodsHeather J. Burke0Francesca Kerton1Centre for Aquaculture and Seafood Development, Fisheries and Marine Institute, Memorial University of Newfoundland, St. John’s, NL A1C 5R3, CanadaDepartment of Chemistry, Memorial University of Newfoundland, St. John’s, NL A1C 5S7, CanadaGreen extraction methods using a combination of mechanical, enzymatic, and green chemical treatments were evaluated for the sequential extraction of carotenoid pigments, protein, and chitin from crab processing discards. Key objectives included avoiding the use of hazardous chemical solvents, conducting as close to a 100% green extraction as possible, and developing simple processes to facilitate implementation into processing plants without the need for complicated and expensive equipment. Three crab bio-products were obtained: pigmented vegetable oil, pigmented protein powder, and chitin. Carotenoid extractions were performed using vegetable oils (corn, canola, and sunflower oils), giving between 24.85% and 37.93% astaxanthin recovery. Citric acid was used to demineralize the remaining material and afforded a pigmented protein powder. Three different proteases were used to deproteinate and isolate chitin in yields between 17.06% and 19.15%. The chitin was still highly colored and therefore decolorization was attempted using hydrogen peroxide. Characterization studies were conducted on each of the crab bio-products isolated including powder X-ray diffraction analysis on the chitin (80.18% crystallinity index, CI, achieved using green methods). Overall, three valuable bio-products could be obtained but further research is needed to obtain pigment-free chitin in an environmentally friendly manner.https://www.mdpi.com/1660-3397/21/6/366snow crabprocessing discardseco-friendlygreen extractionvegetable oilastaxanthin |
| spellingShingle | Heather J. Burke Francesca Kerton Sequential Extraction of Valuable Bio-Products from Snow Crab (<i>Chionoecetes opilio</i>) Processing Discards Using Eco-Friendly Methods Marine Drugs snow crab processing discards eco-friendly green extraction vegetable oil astaxanthin |
| title | Sequential Extraction of Valuable Bio-Products from Snow Crab (<i>Chionoecetes opilio</i>) Processing Discards Using Eco-Friendly Methods |
| title_full | Sequential Extraction of Valuable Bio-Products from Snow Crab (<i>Chionoecetes opilio</i>) Processing Discards Using Eco-Friendly Methods |
| title_fullStr | Sequential Extraction of Valuable Bio-Products from Snow Crab (<i>Chionoecetes opilio</i>) Processing Discards Using Eco-Friendly Methods |
| title_full_unstemmed | Sequential Extraction of Valuable Bio-Products from Snow Crab (<i>Chionoecetes opilio</i>) Processing Discards Using Eco-Friendly Methods |
| title_short | Sequential Extraction of Valuable Bio-Products from Snow Crab (<i>Chionoecetes opilio</i>) Processing Discards Using Eco-Friendly Methods |
| title_sort | sequential extraction of valuable bio products from snow crab i chionoecetes opilio i processing discards using eco friendly methods |
| topic | snow crab processing discards eco-friendly green extraction vegetable oil astaxanthin |
| url | https://www.mdpi.com/1660-3397/21/6/366 |
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