Synthesis of a Nanostructure Molecularly Imprinted Copolymer for Separation of Antifungal Bioactive Di-(2-Ethylhexyl) Phthalate from Biocontrol Fungi Metabolites
Among biocontrol fungi, Trichoderma species produce a wide range of bioactive compounds with antifungal activities. In this study, Di-(2-Ethylhexyl) Phthalate (DEHP) is identified via gas chromatography-mass spectrometry (GC-MS) device in Trichoderma atroviridae (1-3) secondary metabolites and its a...
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| Format: | Article |
| Language: | English |
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Iranian Environmental Mutagen Society
2021-10-01
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| Series: | Journal of Water and Environmental Nanotechnology |
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| Online Access: | http://www.jwent.net/article_248289_90bdb4af0f81b79a48118376da3c9af3.pdf |
| _version_ | 1797836701456924672 |
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| author | Mitra Madani Gargari Kamran Rahnama Maede Shahiri Tabarestani |
| author_facet | Mitra Madani Gargari Kamran Rahnama Maede Shahiri Tabarestani |
| author_sort | Mitra Madani Gargari |
| collection | DOAJ |
| description | Among biocontrol fungi, Trichoderma species produce a wide range of bioactive compounds with antifungal activities. In this study, Di-(2-Ethylhexyl) Phthalate (DEHP) is identified via gas chromatography-mass spectrometry (GC-MS) device in Trichoderma atroviridae (1-3) secondary metabolites and its antifungal effectiveness is confirmed. An eco-friendly approach for the extraction of DEHP is carried out by a nanoporous molecularly imprinted methacrylic acid-based network copolymer as a solid sorbent. Molecularly imprinted polymers (MIPs) are synthesized by precipitation polymerization using DEHP as a template, methacrylic acid (MAA) as a functional monomer and trimethylolpropane trimethacrylate (TRIM) as a cross-linker with molecular ratio (1: 4: 8). After the removal of DEHP, the nanoporous polymer can recognize and rebind specifically the same or structurally very similar molecules. The synthesized MIPs exhibit a suitable tendency to absorb the template with the highest binding capacity of 300 mg/g for DEHP in n-Hexane solvent as a solid phase extraction (SPE) system. The measured particle size of the MIPs with dynamic light scattering (DLS) is reported 75.38 nm. In addition, the porosity of the MIPs is evaluated by nitrogen gas adsorption/desorption using Brouneur Emmet Teller (BET) analysis. Results shows that nanoporous MIPs with an average pore diameter of 2.70 nm and a specific surface area of 309 (cm3/g) are achieved. According to the above-mentioned results, nanoporous MIPs can be considered as an acceptable candidate for separation of the antifungal bioactive compounds (natural fungicide) such as DEHP as an eco-friendly method to replace chemical pesticides. |
| first_indexed | 2024-04-09T15:14:15Z |
| format | Article |
| id | doaj.art-b21c68515593463685d398e285fb8e65 |
| institution | Directory Open Access Journal |
| issn | 2476-7204 2476-6615 |
| language | English |
| last_indexed | 2024-04-09T15:14:15Z |
| publishDate | 2021-10-01 |
| publisher | Iranian Environmental Mutagen Society |
| record_format | Article |
| series | Journal of Water and Environmental Nanotechnology |
| spelling | doaj.art-b21c68515593463685d398e285fb8e652023-04-30T07:03:46ZengIranian Environmental Mutagen SocietyJournal of Water and Environmental Nanotechnology2476-72042476-66152021-10-016437938410.22090/jwent.2021.538642.1429248289Synthesis of a Nanostructure Molecularly Imprinted Copolymer for Separation of Antifungal Bioactive Di-(2-Ethylhexyl) Phthalate from Biocontrol Fungi MetabolitesMitra Madani Gargari0Kamran Rahnama1Maede Shahiri Tabarestani2Department of Plant Protection, Gorgan branch, Islamic Azad University, Gorgan, IranDepartment of Plant Protection, Gorgan branch, Islamic Azad University, Gorgan, IranAssistant Professor, Department of Agriculture, Payame Noor University, Tehran, Iran.Among biocontrol fungi, Trichoderma species produce a wide range of bioactive compounds with antifungal activities. In this study, Di-(2-Ethylhexyl) Phthalate (DEHP) is identified via gas chromatography-mass spectrometry (GC-MS) device in Trichoderma atroviridae (1-3) secondary metabolites and its antifungal effectiveness is confirmed. An eco-friendly approach for the extraction of DEHP is carried out by a nanoporous molecularly imprinted methacrylic acid-based network copolymer as a solid sorbent. Molecularly imprinted polymers (MIPs) are synthesized by precipitation polymerization using DEHP as a template, methacrylic acid (MAA) as a functional monomer and trimethylolpropane trimethacrylate (TRIM) as a cross-linker with molecular ratio (1: 4: 8). After the removal of DEHP, the nanoporous polymer can recognize and rebind specifically the same or structurally very similar molecules. The synthesized MIPs exhibit a suitable tendency to absorb the template with the highest binding capacity of 300 mg/g for DEHP in n-Hexane solvent as a solid phase extraction (SPE) system. The measured particle size of the MIPs with dynamic light scattering (DLS) is reported 75.38 nm. In addition, the porosity of the MIPs is evaluated by nitrogen gas adsorption/desorption using Brouneur Emmet Teller (BET) analysis. Results shows that nanoporous MIPs with an average pore diameter of 2.70 nm and a specific surface area of 309 (cm3/g) are achieved. According to the above-mentioned results, nanoporous MIPs can be considered as an acceptable candidate for separation of the antifungal bioactive compounds (natural fungicide) such as DEHP as an eco-friendly method to replace chemical pesticides.http://www.jwent.net/article_248289_90bdb4af0f81b79a48118376da3c9af3.pdfantifungaldehpmolecularly imprinted polymersnanostructureseparation |
| spellingShingle | Mitra Madani Gargari Kamran Rahnama Maede Shahiri Tabarestani Synthesis of a Nanostructure Molecularly Imprinted Copolymer for Separation of Antifungal Bioactive Di-(2-Ethylhexyl) Phthalate from Biocontrol Fungi Metabolites Journal of Water and Environmental Nanotechnology antifungal dehp molecularly imprinted polymers nanostructure separation |
| title | Synthesis of a Nanostructure Molecularly Imprinted Copolymer for Separation of Antifungal Bioactive Di-(2-Ethylhexyl) Phthalate from Biocontrol Fungi Metabolites |
| title_full | Synthesis of a Nanostructure Molecularly Imprinted Copolymer for Separation of Antifungal Bioactive Di-(2-Ethylhexyl) Phthalate from Biocontrol Fungi Metabolites |
| title_fullStr | Synthesis of a Nanostructure Molecularly Imprinted Copolymer for Separation of Antifungal Bioactive Di-(2-Ethylhexyl) Phthalate from Biocontrol Fungi Metabolites |
| title_full_unstemmed | Synthesis of a Nanostructure Molecularly Imprinted Copolymer for Separation of Antifungal Bioactive Di-(2-Ethylhexyl) Phthalate from Biocontrol Fungi Metabolites |
| title_short | Synthesis of a Nanostructure Molecularly Imprinted Copolymer for Separation of Antifungal Bioactive Di-(2-Ethylhexyl) Phthalate from Biocontrol Fungi Metabolites |
| title_sort | synthesis of a nanostructure molecularly imprinted copolymer for separation of antifungal bioactive di 2 ethylhexyl phthalate from biocontrol fungi metabolites |
| topic | antifungal dehp molecularly imprinted polymers nanostructure separation |
| url | http://www.jwent.net/article_248289_90bdb4af0f81b79a48118376da3c9af3.pdf |
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