Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study
The gut microbiota promotes gastrointestinal health in humans; however, the effect of probiotics on the gut microbiota of healthy adults has not been documented clearly. This placebo-controlled study was conducted to assess the effect of Lacticaseibacillus rhamnosus LRa05 supplementation on the gut...
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1105694/full |
| _version_ | 1797871323498676224 |
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| author | Zhonghui Gai Yao Dong Fei Xu Fei Xu Junli Zhang Yujiao Yang Yujiao Yang Yuwen Wang Yuwen Wang |
| author_facet | Zhonghui Gai Yao Dong Fei Xu Fei Xu Junli Zhang Yujiao Yang Yujiao Yang Yuwen Wang Yuwen Wang |
| author_sort | Zhonghui Gai |
| collection | DOAJ |
| description | The gut microbiota promotes gastrointestinal health in humans; however, the effect of probiotics on the gut microbiota of healthy adults has not been documented clearly. This placebo-controlled study was conducted to assess the effect of Lacticaseibacillus rhamnosus LRa05 supplementation on the gut microbiota of healthy adults. The subjects (N = 100) were randomized 1:1 to receive (1) maltodextrin (placebo, CTL group) and (2) maltodextrin + strain LRa05 (1 × 1010 colony-forming units/day, LRa05 group). The duration of the intervention was 4 weeks, and changes in the gut microbiota from before to after the intervention were investigated using 16S rRNA high-throughput sequencing. In terms of alpha diversity, no significant difference in the composition of the gut microbiota was found between the LRa05 and CTL groups. 16S rRNA sequencing analysis showed that the relative abundance of Lacticaseibacillus significantly increased after supplementation with LRa05. Furthermore, a decreasing trend in the abundance of Sellimonas and a significant decrease in the salmonella infection pathway were observed in the LRa05 group compared with the CTL group. These findings indicate the potential of LRa05 to colonize the human gut and reduce the abundance of harmful bacteria in the microbiota. |
| first_indexed | 2024-04-10T00:42:07Z |
| format | Article |
| id | doaj.art-b21f370707b94a84a126b2943c72f4ce |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-10T00:42:07Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-b21f370707b94a84a126b2943c72f4ce2023-03-14T05:44:32ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-03-011010.3389/fnut.2023.11056941105694Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled studyZhonghui Gai0Yao Dong1Fei Xu2Fei Xu3Junli Zhang4Yujiao Yang5Yujiao Yang6Yuwen Wang7Yuwen Wang8Department of Research and Development, Wecare-Bio Probiotics (Suzhou) Co., Ltd., Suzhou, ChinaDepartment of Research and Development, Wecare-Bio Probiotics (Suzhou) Co., Ltd., Suzhou, ChinaCollege of Food Science and Technology, Henan University of Technology, Zhengzhou, ChinaHenan Province Wheat-Flour Staple Food Engineering Technology Research Centre, Zhengzhou, ChinaDepartment of Research and Development, Wecare-Bio Probiotics (Suzhou) Co., Ltd., Suzhou, ChinaCollege of Food Science and Technology, Henan University of Technology, Zhengzhou, ChinaHenan Province Wheat-Flour Staple Food Engineering Technology Research Centre, Zhengzhou, ChinaCollege of Food Science and Technology, Henan University of Technology, Zhengzhou, ChinaHenan Province Wheat-Flour Staple Food Engineering Technology Research Centre, Zhengzhou, ChinaThe gut microbiota promotes gastrointestinal health in humans; however, the effect of probiotics on the gut microbiota of healthy adults has not been documented clearly. This placebo-controlled study was conducted to assess the effect of Lacticaseibacillus rhamnosus LRa05 supplementation on the gut microbiota of healthy adults. The subjects (N = 100) were randomized 1:1 to receive (1) maltodextrin (placebo, CTL group) and (2) maltodextrin + strain LRa05 (1 × 1010 colony-forming units/day, LRa05 group). The duration of the intervention was 4 weeks, and changes in the gut microbiota from before to after the intervention were investigated using 16S rRNA high-throughput sequencing. In terms of alpha diversity, no significant difference in the composition of the gut microbiota was found between the LRa05 and CTL groups. 16S rRNA sequencing analysis showed that the relative abundance of Lacticaseibacillus significantly increased after supplementation with LRa05. Furthermore, a decreasing trend in the abundance of Sellimonas and a significant decrease in the salmonella infection pathway were observed in the LRa05 group compared with the CTL group. These findings indicate the potential of LRa05 to colonize the human gut and reduce the abundance of harmful bacteria in the microbiota.https://www.frontiersin.org/articles/10.3389/fnut.2023.1105694/fullLacticaseibacillus rhamnosusgut microbiotaprobiotichealthy young volunteersa placebo-controlled study |
| spellingShingle | Zhonghui Gai Yao Dong Fei Xu Fei Xu Junli Zhang Yujiao Yang Yujiao Yang Yuwen Wang Yuwen Wang Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study Frontiers in Nutrition Lacticaseibacillus rhamnosus gut microbiota probiotic healthy young volunteers a placebo-controlled study |
| title | Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study |
| title_full | Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study |
| title_fullStr | Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study |
| title_full_unstemmed | Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study |
| title_short | Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study |
| title_sort | changes in the gut microbiota composition of healthy young volunteers after administration of lacticaseibacillus rhamnosus lra05 a placebo controlled study |
| topic | Lacticaseibacillus rhamnosus gut microbiota probiotic healthy young volunteers a placebo-controlled study |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1105694/full |
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