Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model
Abstract Objective To assess the viability of bovine ovarian tissue after cryopreservation through either slow freezing or vitrification, and to compare it to that of control tissue by performing morphological analyses. Methods The study included 20 bovine ovarian cortex fragments that were divi...
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| Format: | Article |
| Language: | English |
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Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
2016-07-01
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| Series: | Revista Brasileira de Ginecologia e Obstetrícia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032016000700333&tlng=en |
| _version_ | 1819261339454930944 |
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| author | Ana Luisa Menezes Campos Janaína de Souza Guedes Jhenifer Klienchem Rodrigues Walter Antônio Prata Pace Renato Rinco Fontoura João Pedro Junqueira Caetano Ricardo Mello Marinho |
| author_facet | Ana Luisa Menezes Campos Janaína de Souza Guedes Jhenifer Klienchem Rodrigues Walter Antônio Prata Pace Renato Rinco Fontoura João Pedro Junqueira Caetano Ricardo Mello Marinho |
| author_sort | Ana Luisa Menezes Campos |
| collection | DOAJ |
| description | Abstract Objective To assess the viability of bovine ovarian tissue after cryopreservation through either slow freezing or vitrification, and to compare it to that of control tissue by performing morphological analyses. Methods The study included 20 bovine ovarian cortex fragments that were divided into control, vitrification, and slow freezing groups. Each group consisted of four fragments of the same ovary, two fixed without cultivation, and two fixed with cultivation. Tissues were evaluated based on follicular morphology immediately after heating and after 7 days of culture, and compared with the control group. Results A total of 240 fragments were analyzed, generating a sample of 1,344 follicles without cultivation and 552 with cultivation. When the non-cultivated samples were classified as non-atretic follicles, 572 were found in the control group, 289 in the vitrification group, and 373 in the slow freezing group, showing no significant differences. When classified as atretic, 46 follicles were found in the control group, 23 in the vitrification group, and 41 in the slow freezing group, also showing no statistical difference. In the post-culture sample, an evolution of the follicular stages could be observed. This finding was important to support that the follicles considered non-atretic in the non-cultivated group were actually viable in the morphological evaluation. Conclusion With no differences between the protocols, vitrification was shown to be an advanced and alternative method for patients who will undergo treatments that |
| first_indexed | 2024-12-23T19:40:14Z |
| format | Article |
| id | doaj.art-b21f95aed0d54da69c4e0ca0dcf373c1 |
| institution | Directory Open Access Journal |
| issn | 0100-7203 |
| language | English |
| last_indexed | 2024-12-23T19:40:14Z |
| publishDate | 2016-07-01 |
| publisher | Federação Brasileira das Sociedades de Ginecologia e Obstetrícia |
| record_format | Article |
| series | Revista Brasileira de Ginecologia e Obstetrícia |
| spelling | doaj.art-b21f95aed0d54da69c4e0ca0dcf373c12022-12-21T17:33:41ZengFederação Brasileira das Sociedades de Ginecologia e ObstetríciaRevista Brasileira de Ginecologia e Obstetrícia0100-72032016-07-0138733333910.1055/s-0036-1586258Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine ModelAna Luisa Menezes CamposJanaína de Souza GuedesJhenifer Klienchem RodriguesWalter Antônio Prata PaceRenato Rinco FontouraJoão Pedro Junqueira CaetanoRicardo Mello MarinhoAbstract Objective To assess the viability of bovine ovarian tissue after cryopreservation through either slow freezing or vitrification, and to compare it to that of control tissue by performing morphological analyses. Methods The study included 20 bovine ovarian cortex fragments that were divided into control, vitrification, and slow freezing groups. Each group consisted of four fragments of the same ovary, two fixed without cultivation, and two fixed with cultivation. Tissues were evaluated based on follicular morphology immediately after heating and after 7 days of culture, and compared with the control group. Results A total of 240 fragments were analyzed, generating a sample of 1,344 follicles without cultivation and 552 with cultivation. When the non-cultivated samples were classified as non-atretic follicles, 572 were found in the control group, 289 in the vitrification group, and 373 in the slow freezing group, showing no significant differences. When classified as atretic, 46 follicles were found in the control group, 23 in the vitrification group, and 41 in the slow freezing group, also showing no statistical difference. In the post-culture sample, an evolution of the follicular stages could be observed. This finding was important to support that the follicles considered non-atretic in the non-cultivated group were actually viable in the morphological evaluation. Conclusion With no differences between the protocols, vitrification was shown to be an advanced and alternative method for patients who will undergo treatments thathttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032016000700333&tlng=enovarian tissuevitrificationslow freezingfertility |
| spellingShingle | Ana Luisa Menezes Campos Janaína de Souza Guedes Jhenifer Klienchem Rodrigues Walter Antônio Prata Pace Renato Rinco Fontoura João Pedro Junqueira Caetano Ricardo Mello Marinho Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model Revista Brasileira de Ginecologia e Obstetrícia ovarian tissue vitrification slow freezing fertility |
| title | Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model |
| title_full | Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model |
| title_fullStr | Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model |
| title_full_unstemmed | Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model |
| title_short | Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model |
| title_sort | comparison between slow freezing and vitrification in terms of ovarian tissue viability in a bovine model |
| topic | ovarian tissue vitrification slow freezing fertility |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032016000700333&tlng=en |
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