A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019
Water bodies worldwide have proven to be vast reservoirs of clinically significant antibiotic resistant organisms. Contamination of waters by anthropogenic discharges is a significant contributor to the widespread dissemination of antibiotic resistance. The aim of this research was to investigate mu...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S016041202100091X |
| _version_ | 1818329425370939392 |
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| author | Brigid Hooban Kelly Fitzhenry Niamh Cahill Aoife Joyce Louise O' Connor James E. Bray Sylvain Brisse Virginie Passet Raza Abbas Syed Martin Cormican Dearbháile Morris |
| author_facet | Brigid Hooban Kelly Fitzhenry Niamh Cahill Aoife Joyce Louise O' Connor James E. Bray Sylvain Brisse Virginie Passet Raza Abbas Syed Martin Cormican Dearbháile Morris |
| author_sort | Brigid Hooban |
| collection | DOAJ |
| description | Water bodies worldwide have proven to be vast reservoirs of clinically significant antibiotic resistant organisms. Contamination of waters by anthropogenic discharges is a significant contributor to the widespread dissemination of antibiotic resistance. The aim of this research was to investigate multiple different anthropogenic sources on a national scale for the role they play in the environmental propagation of antibiotic resistance. A total of 39 water and 25 sewage samples were collected across four local authority areas in the West, East and South of Ireland. In total, 211 Enterobacterales were isolated (139 water, 72 sewage) and characterised. A subset of isolates (n=60) were chosen for whole genome sequencing. Direct comparisons of the water versus sewage isolate collections revealed a higher percentage of sewage isolates displayed resistance to cefoxitin (46%) and ertapenem (32%), while a higher percentage of water isolates displayed resistance to tetracycline (55%) and ciprofloxacin (71%). Half of all isolates displayed extended spectrum beta-lactamase (ESBL) production phenotypically (n = 105/211; 50%), with blaCTX-M detected in 99/105 isolates by PCR. Carbapenemase genes were identified in 11 isolates (6 sewage, 5 water). The most common variant was blaOXA-48 (n=6), followed by blaNDM-5 (n=2) and blaKPC-2 (n=2). Whole genome sequencing analysis revealed numerous different sequence types in circulation in both waters and sewage including E. coli ST131 (n=15), ST38 (n=8), ST10 (n=4) along with Klebsiella ST405 (n=3) and ST11 (n=2). Core genome MLST (cgMLST) comparisons uncovered three highly similar Klebsiella isolates originating from hospital sewage and two nearby waters. The Klebsiella isolates from an estuary and seawater displayed 99.1% and 98.8% cgMLST identity to the hospital sewage isolate respectively. In addition, three pairs of E. coli isolates from different waters also revealed cgMLST similarities, indicating widespread dissemination and persistence of certain strains in the aquatic environment. These findings highlight the need for routine monitoring of water bodies used for recreational and drinking purposes for the presence of multi-drug resistant organisms. |
| first_indexed | 2024-12-13T12:47:51Z |
| format | Article |
| id | doaj.art-b2228567cf234c76abb6d544f02d0996 |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-12-13T12:47:51Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-b2228567cf234c76abb6d544f02d09962022-12-21T23:45:26ZengElsevierEnvironment International0160-41202021-07-01152106466A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019Brigid Hooban0Kelly Fitzhenry1Niamh Cahill2Aoife Joyce3Louise O' Connor4James E. Bray5Sylvain Brisse6Virginie Passet7Raza Abbas Syed8Martin Cormican9Dearbháile Morris10Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway; Centre for One Health, Ryan Institute, National University of Ireland, Galway; Corresponding author.Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway; Centre for One Health, Ryan Institute, National University of Ireland, GalwayAntimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway; Centre for One Health, Ryan Institute, National University of Ireland, GalwayAntimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway; Centre for One Health, Ryan Institute, National University of Ireland, GalwayAntimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway; Centre for One Health, Ryan Institute, National University of Ireland, GalwayDepartment of Zoology, University of Oxford, Oxford, United KingdomBiodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, FranceBiodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, FranceAntimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, GalwayAntimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway; Centre for One Health, Ryan Institute, National University of Ireland, Galway; Health Service Executive, IrelandAntimicrobial Resistance and Microbial Ecology Group, School of Medicine, National University of Ireland, Galway; Centre for One Health, Ryan Institute, National University of Ireland, GalwayWater bodies worldwide have proven to be vast reservoirs of clinically significant antibiotic resistant organisms. Contamination of waters by anthropogenic discharges is a significant contributor to the widespread dissemination of antibiotic resistance. The aim of this research was to investigate multiple different anthropogenic sources on a national scale for the role they play in the environmental propagation of antibiotic resistance. A total of 39 water and 25 sewage samples were collected across four local authority areas in the West, East and South of Ireland. In total, 211 Enterobacterales were isolated (139 water, 72 sewage) and characterised. A subset of isolates (n=60) were chosen for whole genome sequencing. Direct comparisons of the water versus sewage isolate collections revealed a higher percentage of sewage isolates displayed resistance to cefoxitin (46%) and ertapenem (32%), while a higher percentage of water isolates displayed resistance to tetracycline (55%) and ciprofloxacin (71%). Half of all isolates displayed extended spectrum beta-lactamase (ESBL) production phenotypically (n = 105/211; 50%), with blaCTX-M detected in 99/105 isolates by PCR. Carbapenemase genes were identified in 11 isolates (6 sewage, 5 water). The most common variant was blaOXA-48 (n=6), followed by blaNDM-5 (n=2) and blaKPC-2 (n=2). Whole genome sequencing analysis revealed numerous different sequence types in circulation in both waters and sewage including E. coli ST131 (n=15), ST38 (n=8), ST10 (n=4) along with Klebsiella ST405 (n=3) and ST11 (n=2). Core genome MLST (cgMLST) comparisons uncovered three highly similar Klebsiella isolates originating from hospital sewage and two nearby waters. The Klebsiella isolates from an estuary and seawater displayed 99.1% and 98.8% cgMLST identity to the hospital sewage isolate respectively. In addition, three pairs of E. coli isolates from different waters also revealed cgMLST similarities, indicating widespread dissemination and persistence of certain strains in the aquatic environment. These findings highlight the need for routine monitoring of water bodies used for recreational and drinking purposes for the presence of multi-drug resistant organisms.http://www.sciencedirect.com/science/article/pii/S016041202100091XAntibiotic ResistanceAquatic EnvironmentWastewaterCarbapenemaseExtended Spectrum Beta-Lactamase |
| spellingShingle | Brigid Hooban Kelly Fitzhenry Niamh Cahill Aoife Joyce Louise O' Connor James E. Bray Sylvain Brisse Virginie Passet Raza Abbas Syed Martin Cormican Dearbháile Morris A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019 Environment International Antibiotic Resistance Aquatic Environment Wastewater Carbapenemase Extended Spectrum Beta-Lactamase |
| title | A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019 |
| title_full | A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019 |
| title_fullStr | A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019 |
| title_full_unstemmed | A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019 |
| title_short | A Point Prevalence Survey of Antibiotic Resistance in the Irish Environment, 2018–2019 |
| title_sort | point prevalence survey of antibiotic resistance in the irish environment 2018 2019 |
| topic | Antibiotic Resistance Aquatic Environment Wastewater Carbapenemase Extended Spectrum Beta-Lactamase |
| url | http://www.sciencedirect.com/science/article/pii/S016041202100091X |
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