Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysis
Abstract Background Hepatitis C virus (HCV) infection remains a major public health problem in Chinese mainland. Investigation of the distribution of genotypes contributed to the prevention, diagnosis and treatment of HCV infection. Therefore, we conducted a study on the distribution of HCV genotype...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | Infectious Diseases of Poverty |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40249-023-01106-y |
| _version_ | 1827899564747653120 |
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| author | Qiao Tang Zhiwei Chen Hu Li Li Zhang Mingli Peng Yi Zeng Xiaoqing Liu Zubi Liu Peng Hu |
| author_facet | Qiao Tang Zhiwei Chen Hu Li Li Zhang Mingli Peng Yi Zeng Xiaoqing Liu Zubi Liu Peng Hu |
| author_sort | Qiao Tang |
| collection | DOAJ |
| description | Abstract Background Hepatitis C virus (HCV) infection remains a major public health problem in Chinese mainland. Investigation of the distribution of genotypes contributed to the prevention, diagnosis and treatment of HCV infection. Therefore, we conducted a study on the distribution of HCV genotypes and phylogenetic analysis to provide an up-to-date understanding of the molecular epidemiology of genotypes in Chinese mainland. Methods Our retrospective multicenter study enrolled 11,008 samples collected between August 2018 and July 2019 from 29 provinces/municipalities (Beijing, Hebei, Inner Mongolia, Shanxi, Tianjin, Gansu, Ningxia, Shaanxi, Xinjiang, Heilongjiang, Jilin Liaoning, Henan, Hubei Hunan, Anhui, Fujian, Jiangsu, Jiangxi, Shandong, Shanghai Zhejiang, Guangdong, Guangxi, Hainan, Chongqing, Guizhou, Sichuan and Yunnan). Phylogenetic analysis of each subtype was performed to infer the evolutionary relationship of sequences from diverse regions. Two independent samples t tests were used for the comparison of continuous variables, and chi-square tests were used for the comparison of categorical variables. Results Four genotypes (1, 2, 3 and 6) were found, including 14 subtypes. HCV genotype 1 was dominant, accounting for 49.2%, followed by genotypes 2, 3 and 6, accounting for 22.4%, 16.4%, and 11.9%, respectively. Additionally, the top five subtypes were 1b, 2a, 3b, 6a and 3a. Proportions of genotypes 1 and 2 decreased while genotypes 3 and 6 increased over past years (P < 0.001). Genotypes 3 and 6 were concentrated in the population aged 30 to 50 years, and male carriers had lower proportions of subtypes 1b and 2a than female carriers (P < 0.01). Genotypes 3 and 6 were more prevalent in southern parts of Chinese mainland. Nationwide spreads of subtypes 1b and 2a were associated with sequences from northern parts of Chinese mainland, while subtypes 3a, 3b and 6a were associated with sequences from southern parts of Chinese mainland. Conclusions HCV subtypes 1b and 2a remained the most common subtypes in Chinese mainland, and their proportions decreased over the past years, while the proportions of genotypes 3 and 6 increased. Our investigation provided an accurate epidemiological picture of the circulating viral strains in Chinese mainland, contributing to the prevention, diagnosis and treatment of HCV infection. Trial registration: Not applicable. |
| first_indexed | 2024-03-12T23:19:41Z |
| format | Article |
| id | doaj.art-b223ad3881b946a8a69fe2256b2c10bc |
| institution | Directory Open Access Journal |
| issn | 2049-9957 |
| language | English |
| last_indexed | 2024-03-12T23:19:41Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Diseases of Poverty |
| spelling | doaj.art-b223ad3881b946a8a69fe2256b2c10bc2023-07-16T11:31:32ZengBMCInfectious Diseases of Poverty2049-99572023-07-0112111210.1186/s40249-023-01106-yMolecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysisQiao Tang0Zhiwei Chen1Hu Li2Li Zhang3Mingli Peng4Yi Zeng5Xiaoqing Liu6Zubi Liu7Peng Hu8Department of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Chinese Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical UniversityDepartment of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Chinese Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical UniversityDepartment of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Chinese Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical UniversityDepartment of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Chinese Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical UniversityDepartment of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Chinese Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical UniversityDepartment of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Chinese Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical UniversityDepartment of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Chinese Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical UniversityKey Laboratory of Digital Technology in Medical Diagnostics of Zhejiang ProvinceDepartment of Infectious Diseases, Key Laboratory of Molecular Biology for Infectious Diseases (Chinese Ministry of Education), Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical UniversityAbstract Background Hepatitis C virus (HCV) infection remains a major public health problem in Chinese mainland. Investigation of the distribution of genotypes contributed to the prevention, diagnosis and treatment of HCV infection. Therefore, we conducted a study on the distribution of HCV genotypes and phylogenetic analysis to provide an up-to-date understanding of the molecular epidemiology of genotypes in Chinese mainland. Methods Our retrospective multicenter study enrolled 11,008 samples collected between August 2018 and July 2019 from 29 provinces/municipalities (Beijing, Hebei, Inner Mongolia, Shanxi, Tianjin, Gansu, Ningxia, Shaanxi, Xinjiang, Heilongjiang, Jilin Liaoning, Henan, Hubei Hunan, Anhui, Fujian, Jiangsu, Jiangxi, Shandong, Shanghai Zhejiang, Guangdong, Guangxi, Hainan, Chongqing, Guizhou, Sichuan and Yunnan). Phylogenetic analysis of each subtype was performed to infer the evolutionary relationship of sequences from diverse regions. Two independent samples t tests were used for the comparison of continuous variables, and chi-square tests were used for the comparison of categorical variables. Results Four genotypes (1, 2, 3 and 6) were found, including 14 subtypes. HCV genotype 1 was dominant, accounting for 49.2%, followed by genotypes 2, 3 and 6, accounting for 22.4%, 16.4%, and 11.9%, respectively. Additionally, the top five subtypes were 1b, 2a, 3b, 6a and 3a. Proportions of genotypes 1 and 2 decreased while genotypes 3 and 6 increased over past years (P < 0.001). Genotypes 3 and 6 were concentrated in the population aged 30 to 50 years, and male carriers had lower proportions of subtypes 1b and 2a than female carriers (P < 0.01). Genotypes 3 and 6 were more prevalent in southern parts of Chinese mainland. Nationwide spreads of subtypes 1b and 2a were associated with sequences from northern parts of Chinese mainland, while subtypes 3a, 3b and 6a were associated with sequences from southern parts of Chinese mainland. Conclusions HCV subtypes 1b and 2a remained the most common subtypes in Chinese mainland, and their proportions decreased over the past years, while the proportions of genotypes 3 and 6 increased. Our investigation provided an accurate epidemiological picture of the circulating viral strains in Chinese mainland, contributing to the prevention, diagnosis and treatment of HCV infection. Trial registration: Not applicable.https://doi.org/10.1186/s40249-023-01106-yHepatitis C virusGenotypeDistributionPhylogenetic analysisChina |
| spellingShingle | Qiao Tang Zhiwei Chen Hu Li Li Zhang Mingli Peng Yi Zeng Xiaoqing Liu Zubi Liu Peng Hu Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysis Infectious Diseases of Poverty Hepatitis C virus Genotype Distribution Phylogenetic analysis China |
| title | Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysis |
| title_full | Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysis |
| title_fullStr | Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysis |
| title_full_unstemmed | Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysis |
| title_short | Molecular epidemiology of hepatitis C virus genotypes in different geographical regions of Chinese mainland and a phylogenetic analysis |
| title_sort | molecular epidemiology of hepatitis c virus genotypes in different geographical regions of chinese mainland and a phylogenetic analysis |
| topic | Hepatitis C virus Genotype Distribution Phylogenetic analysis China |
| url | https://doi.org/10.1186/s40249-023-01106-y |
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