Combined Fluorescence-Based in Vitro Assay for the Simultaneous Detection of Cell Viability and Alkaline Phosphatase Activity during Osteogenic Differentiation of Osteoblast Precursor Cells

Combined Fluorescence-Based in Vitro Assay for the Simultaneous Detection of Cell Viability and Alkaline Phosphatase Activity during Osteogenic Differentiation of Osteoblast Precursor Cells

Novel bone substitute materials need to be evaluated in terms of their osteogenic differentiation capacity and possible unwanted cytotoxic effects in order to identify promising candidates for the therapy of bone defects. The activity of alkaline phosphatase (ALP) is frequently quantified as an oste...

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Bibliographic Details
Main Authors:	Sebastian Wilkesmann, Fabian Westhauser, Joerg Fellenberg
Format:	Article
Language:	English
Published:	MDPI AG 2020-04-01
Series:	Methods and Protocols
Subjects:	osteogenic differentiation alkaline phosphatase human mesenchymal stromal cell osteoblast precursor cell
Online Access:	https://www.mdpi.com/2409-9279/3/2/30

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