Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stress
Abstract Background Sweetpotato is a typical ‘‘potassium (K+) favoring’’ food crop, which root differentiation process needs a large supply of potassium fertilizer and determine the final root yield. To further understand the regulatory network of the response to low potassium stress, here we analyz...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09939-5 |
| _version_ | 1797350151273054208 |
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| author | Rong Jin Mengxiao Yan Guanghua Li Ming Liu Peng Zhao Zhe Zhang Qiangqiang Zhang Xiaoya Zhu Jing Wang Yongchao Yu Aijun Zhang Jun Yang Zhonghou Tang |
| author_facet | Rong Jin Mengxiao Yan Guanghua Li Ming Liu Peng Zhao Zhe Zhang Qiangqiang Zhang Xiaoya Zhu Jing Wang Yongchao Yu Aijun Zhang Jun Yang Zhonghou Tang |
| author_sort | Rong Jin |
| collection | DOAJ |
| description | Abstract Background Sweetpotato is a typical ‘‘potassium (K+) favoring’’ food crop, which root differentiation process needs a large supply of potassium fertilizer and determine the final root yield. To further understand the regulatory network of the response to low potassium stress, here we analyze physiological and biochemical characteristics, and investigated root transcriptional changes in two sweetpotato genotypes, namely, - K tolerant “Xu32” and - K susceptible“NZ1”. Result We found Xu32 had the higher capability of K+ absorption than NZ1 with better growth performance, higher net photosynthetic rate and higher chlorophyll contents under low potassium stress, and identified 889 differentially expressed genes (DEGs) in Xu32, 634 DEGs in NZ1, 256 common DEGs in both Xu32 and NZ1. The Gene Ontology (GO) term in molecular function enrichment analysis revealed that the DEGs under low K+ stress are predominately involved in catalytic activity, binding, transporter activity and antioxidant activity. Moreover, the more numbers of identified DEGs in Xu32 than that in NZ1 responded to K+-deficiency belong to the process of photosynthesis, carbohydrate metabolism, ion transport, hormone signaling, stress-related and antioxidant system may result in different ability to K+-deficiency tolerance. The unique genes in Xu32 may make a great contribution to enhance low K+ tolerance, and provide useful information for the molecular regulation mechanism of K+-deficiency tolerance in sweetpotato. Conclusions The common and distinct expression pattern between the two sweetpotato genotypes illuminate a complex mechanism response to low potassium exist in sweetpotato. The study provides some candidate genes, which can be used in sweetpotato breeding program for improving low potassium stress tolerance. |
| first_indexed | 2024-03-08T12:40:39Z |
| format | Article |
| id | doaj.art-b22753e4bfe94935b958ea74d672a0ac |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-08T12:40:39Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-b22753e4bfe94935b958ea74d672a0ac2024-01-21T12:11:44ZengBMCBMC Genomics1471-21642024-01-0125111710.1186/s12864-023-09939-5Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stressRong Jin0Mengxiao Yan1Guanghua Li2Ming Liu3Peng Zhao4Zhe Zhang5Qiangqiang Zhang6Xiaoya Zhu7Jing Wang8Yongchao Yu9Aijun Zhang10Jun Yang11Zhonghou Tang12Xuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Gardensishui lifeng food products Co., LtdXuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesXuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesSishui County Agriculture and Rural BureauXuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesXuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesXuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesXuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesXuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesShanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical GardenXuzhou Sweetpotato Research Center, Xuzhou Institute of Agricultural SciencesAbstract Background Sweetpotato is a typical ‘‘potassium (K+) favoring’’ food crop, which root differentiation process needs a large supply of potassium fertilizer and determine the final root yield. To further understand the regulatory network of the response to low potassium stress, here we analyze physiological and biochemical characteristics, and investigated root transcriptional changes in two sweetpotato genotypes, namely, - K tolerant “Xu32” and - K susceptible“NZ1”. Result We found Xu32 had the higher capability of K+ absorption than NZ1 with better growth performance, higher net photosynthetic rate and higher chlorophyll contents under low potassium stress, and identified 889 differentially expressed genes (DEGs) in Xu32, 634 DEGs in NZ1, 256 common DEGs in both Xu32 and NZ1. The Gene Ontology (GO) term in molecular function enrichment analysis revealed that the DEGs under low K+ stress are predominately involved in catalytic activity, binding, transporter activity and antioxidant activity. Moreover, the more numbers of identified DEGs in Xu32 than that in NZ1 responded to K+-deficiency belong to the process of photosynthesis, carbohydrate metabolism, ion transport, hormone signaling, stress-related and antioxidant system may result in different ability to K+-deficiency tolerance. The unique genes in Xu32 may make a great contribution to enhance low K+ tolerance, and provide useful information for the molecular regulation mechanism of K+-deficiency tolerance in sweetpotato. Conclusions The common and distinct expression pattern between the two sweetpotato genotypes illuminate a complex mechanism response to low potassium exist in sweetpotato. The study provides some candidate genes, which can be used in sweetpotato breeding program for improving low potassium stress tolerance.https://doi.org/10.1186/s12864-023-09939-5SweetpotatoGenotypeK+-deficiencyRNA-seqDifferentially expressed genes (DEGs) |
| spellingShingle | Rong Jin Mengxiao Yan Guanghua Li Ming Liu Peng Zhao Zhe Zhang Qiangqiang Zhang Xiaoya Zhu Jing Wang Yongchao Yu Aijun Zhang Jun Yang Zhonghou Tang Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stress BMC Genomics Sweetpotato Genotype K+-deficiency RNA-seq Differentially expressed genes (DEGs) |
| title | Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stress |
| title_full | Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stress |
| title_fullStr | Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stress |
| title_full_unstemmed | Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stress |
| title_short | Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stress |
| title_sort | comparative physiological and transcriptome analysis between potassium deficiency tolerant and sensitive sweetpotato genotypes in response to potassium deficiency stress |
| topic | Sweetpotato Genotype K+-deficiency RNA-seq Differentially expressed genes (DEGs) |
| url | https://doi.org/10.1186/s12864-023-09939-5 |
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