Generation of Marker-Free <i>pbd-2</i> Knock-in Pigs Using the CRISPR/Cas9 and Cre/loxP Systems

Generation of Marker-Free <i>pbd-2</i> Knock-in Pigs Using the CRISPR/Cas9 and Cre/loxP Systems

Porcine β-defensin 2 (PBD-2), expressed by different tissues of pigs, is a multifunctional cationic peptide with antimicrobial, immunomodulatory and growth-promoting abilities. As the latest generation of genome-editing tool, CRISPR/Cas9 system makes it possible to enhance the expression of PBD-2 in...

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Bibliographic Details
Main Authors: Jing Huang, Antian Wang, Chao Huang, Yufan Sun, Bingxiao Song, Rui Zhou, Lu Li
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Genes
Subjects:
porcine β-defensin 2
CRISPR/Cas9
transgenic pigs
antimicrobial peptide
disease-resistant animals
Online Access:https://www.mdpi.com/2073-4425/11/8/951

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